Rictor Antibody (7B3)

Images

 
Western Blot: RICTOR Antibody (7B3) [NBP1-51645] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.
Immunocytochemistry/ Immunofluorescence: RICTOR Antibody (7B3) [NBP1-51645] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: RICTOR Antibody (7B3) [NBP1-51645] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB ...read more
Flow Cytometry: RICTOR Antibody (7B3) [NBP1-51645] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Flow Cytometry: RICTOR Antibody (7B3) [NBP1-51645] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
7B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Rictor Antibody (7B3) Summary

Immunogen
Purified recombinant fragment of human RICTOR expressed in E. coli. [UniProt# Q6R327]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RICTOR
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This RICTOR (7B3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.Use in Immunoprecipitation reported in scientific literature (PMID: 22952709) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
HepG2 Lysate (NBP1-42569)
Publications
Read Publications using
NBP1-51645 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Human, mouse and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Rictor Antibody (7B3)

  • AVO3 homolog
  • AVO3
  • hAVO3
  • KIAA1999DKFZp686B11164
  • mAVO3
  • MGC39830
  • PIA
  • Pianissimo
  • rapamycin-insensitive companion of mTOR
  • Rictor
  • RPTOR independent companion of MTOR, complex 2
  • TORC2-specific protein AVO3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rictor Antibody (NBP1-51645)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
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Human
(1)
Mouse
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rictor Antibody (NBP1-51645) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

PE Labeled NBP1-51645PE

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Bioinformatics Tool for Rictor Antibody (NBP1-51645)

Discover related pathways, diseases and genes to Rictor Antibody (NBP1-51645). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Rictor.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

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Bioinformatics

Gene Symbol RICTOR
Entrez
Uniprot