GBL Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. ...read more
G beta L was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human/Mouse/Rat G beta L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4004) at 10 µg/mL ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GBL Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human G beta L
Met1-Gly327
Accession # Q9BVC4
Specificity
Detects human, mouse, and rat G beta L in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MLST8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF4004 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GBL Antibody [Unconjugated]

  • G protein beta subunit-like
  • gable
  • GbetaL
  • GBL
  • GBLPop3
  • Lst8
  • LST8MGC111011
  • Mammalian lethal with SEC13 protein 8
  • mLST8
  • MTOR associated protein, LST8 homolog (S. cerevisiae)
  • POP3
  • Protein GbetaL
  • target of rapamycin complex subunit LST8
  • TORC subunit LST8
  • WAT1

Background

G-protein beta -subunit-like (G beta L), also known as mLST8, is a subunit of the Target of Rapamycin (TOR) complex. G beta L contains 7 WD-40 repeats, and binds to the kinase domain of TOR in both the Raptor (Rapamycin-sensitive)-and Rictor (Rapamycin-insensitive)-containing complexes. G beta L is necessary for the full activation of the TOR kinase, and phosphorylation of many downstream proteins, including 4E-BP1 and p70S6 kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GBL Antibody (AF4004) (0)

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Review for GBL Antibody (AF4004) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF4004:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot GBL AF4004
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5
reviewed by:
Verified Customer
WB Mouse 10/23/2019
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Summary

ApplicationWestern Blot
Sample TestedAdipose tissue
SpeciesMouse
LotWYE021909A

Comments

CommentsMouse white adipose tissue was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody gbl diluted 1:1000 in 5% BSA 2, before incubation with anti goat secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.

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Video Protocols

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FAQs for GBL Antibody (AF4004) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

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1
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Verified Customer
10/23/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MLST8
Uniprot