Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. ...read more
G beta L was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human/Mouse/Rat G beta L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4004) at 10 µg/mL ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for GBL Antibody [Unconjugated]
G protein beta subunit-like
Mammalian lethal with SEC13 protein 8
MTOR associated protein, LST8 homolog (S. cerevisiae)
target of rapamycin complex subunit LST8
TORC subunit LST8
G-protein beta -subunit-like (G beta L), also known as mLST8, is a subunit of the Target of Rapamycin (TOR) complex. G beta L contains 7 WD-40 repeats, and binds to the kinase domain of TOR in both the Raptor (Rapamycin-sensitive)-and Rictor (Rapamycin-insensitive)-containing complexes. G beta L is necessary for the full activation of the TOR kinase, and phosphorylation of many downstream proteins, including 4E-BP1 and p70S6 kinase.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Mouse white adipose tissue was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody gbl diluted 1:1000 in 5% BSA 2, before incubation with anti goat secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.