RPTOR Antibody (SR1170) - BSA Free

Western Blot: RPTOR Antibody (SR1170) [NBP3-21541] - Western blot analysis using NBP3-21541 in MCF-7 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: SR1170
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPTOR Antibody (SR1170) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human RPTOR (Uniprot #: Q8N122)
• Specificity: Detects endogenous levels of RPTOR
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPTOR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:30
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunoprecipitation 1:30
  • Western Blot 1:500-1:1000
• Theoretical MW: 149 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RPTOR Antibody (SR1170) - BSA Free

• KIAA1303
• KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
• KOG1
• Mip1
• p150 target of rapamycin (TOR)-scaffold protein
• Raptor
• regulatory associated protein of MTOR, complex 1
• regulatory-associated protein of mTOR
• RPTOR

Background

Raptor (regulatory associated protein of TOR) is a conserved 150 kDa adaptor protein composed of a unique conserved region, three HEAT repeats and seven WD-40 repeats (1). Raptor recruits mTOR substrates S6K1 (p70 S6 kinase) and 4E-BP1, and regulates mTOR kinase activity (2-3). Even though Raptor is a positive regulator of mTOR, it has been shown that under nutrient deprivation, the Raptor-mTOR association is stabilized in a manner that inhibits mTOR kinase activity (1). Raptor association with mTOR is required for efficient S6K1 and 4E0BP1 phosphorylation (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPTOR