RPA70 Antibody

Images

 
Western Blot: RPA70 Antibody [NBP1-31382] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody diluted by 1:1000
Immunocytochemistry/ Immunofluorescence: RPA70 Antibody [NBP1-31382] - Analysis of HeLa, using NBP1-31382 at 1:200 dilution.
Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382] - Paraffin-embedded human lung papillary adenocarcinoma. RPA70 antibody [C1C3] diluted at 1:250.
Western Blot: RPA70 Antibody [NBP1-31382] - H1299 (lane A), HeLa S3 (lane B), and Raji (lane C).
Western Blot: RPA70 Antibody [NBP1-31382] - A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Western Blot: RPA70 Antibody [NBP1-31382] - A:H1299 B:HeLa.
Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: RPA70 Antibody [NBP1-31382] - Paraffin-embedded mouse brain. RPA70 antibody [C1C3] diluted at 1:500.
Immunoprecipitation: RPA70 Antibody [NBP1-31382] - Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 ug of RPA70 antibody [C1C3].

Product Details

Summary
Reactivity Hu, Mu, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.4 mg/ml

Order Details

RPA70 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Sheep (93%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPA70 Antibody

  • HSSB
  • MST075
  • MSTP075
  • REPA1RF-A protein 1
  • Replication factor A protein 1
  • replication protein A 70 kDa DNA-binding subunit
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
  • RF-A
  • RP-A
  • RPA70RP-A p70
  • Single-stranded DNA-binding protein

Background

RPA (Replication protein A) is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is a single strand binding protein that is involved in replication, homologous recombination and nucleotide excision and repair. It colocalises with RAD51 on synapsed axes in meiosis, facilitating RAD51 function. Replication protein A acts as an auxiliary protein for DNA polymerases alpha and delta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP1-23017
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA
NB100-93321
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-148
Species: Hu, Mu, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro
NB100-159
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NB100-308
Species: Hu
Applications: WB, Func, ICC/IF, IP
H00084172-M10
Species: Hu
Applications: WB, ELISA, ICC/IF
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-30371
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-22128
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP2-03417
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB100-56585
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-48002
Species: Hu, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-90149
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NB100-464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
AF6696
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP1-31266
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-74457
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP1-31382
Species: Hu, Mu, Bv, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for RPA70 Antibody (NBP1-31382) (0)

There are no publications for RPA70 Antibody (NBP1-31382).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RPA70 Antibody (NBP1-31382) (0)

There are no reviews for RPA70 Antibody (NBP1-31382). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPA70 Antibody (NBP1-31382) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional RPA70 Products

Bioinformatics Tool for RPA70 Antibody (NBP1-31382)

Discover related pathways, diseases and genes to RPA70 Antibody (NBP1-31382). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RPA70 Antibody (NBP1-31382)

Discover more about diseases related to RPA70 Antibody (NBP1-31382).
 

Pathways for RPA70 Antibody (NBP1-31382)

View related products by pathway.

PTMs for RPA70 Antibody (NBP1-31382)

Learn more about PTMs related to RPA70 Antibody (NBP1-31382).
 

Research Areas for RPA70 Antibody (NBP1-31382)

Find related products by research area.

Blogs on RPA70

There are no specific blogs for RPA70, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RPA70 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol RPA1