RPA70 Antibody

Images

 
Western Blot: RPA70 Antibody [NB100-2204] - Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa and (15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry: RPA70 Antibody [NB100-2204] - Samples: FFPE serial sections of mouse teratoma. Antibodies: Affinity purified rabbit anti- Cat. No. Lot2 used at a dilution of 1:1,000 (1 ug/ml) (left) and Cat. No. ...read more
Western Blot: RPA70 Antibody [NB100-2204] - Whole cell lysate (10, 50 and 100 ug/lane in lanes 1, 2 and 3, respectively) from HeLa cells. Antibody used at the indicated concentrations.
Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - FFPE section of human seminoma. Affinity purified rabbit anti-RPA70 used at a dilution of 1:250.
Proximity Ligation Assay: RPA70 Antibody [NB100-2204] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human RPA70 (NB100-2204). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RPA70 Antibody [NB100-2204] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human RPA70 (NB100-2204). Image merged from DAPI (2ms) and Texas ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

RPA70 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human replication protein A1, 70kDa using the numbering given in entry NP_002936.1 (GeneID 6117).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:25000
  • Immunohistochemistry 1:10-2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Proximity Ligation Assay 1:1000-1:20000
Positive Control
RPA70 Lysate (NBL1-15488)
Publications
Read Publications using NB100-2204.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPA70 Antibody

  • HSSB
  • MST075
  • MSTP075
  • REPA1RF-A protein 1
  • Replication factor A protein 1
  • replication protein A 70 kDa DNA-binding subunit
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
  • RF-A
  • RP-A
  • RPA70RP-A p70
  • Single-stranded DNA-binding protein

Background

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA1, the 70 kDa subunit, is an ssDNA binding protein that has been shown to be phosphorylated by ATM and ATR in response to DNA damage. In addition to a role in MMR, RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication. Alternate names for RPA70 include replication protein A 70 kDa DNA-binding subunit, RP-A, RF-A, Replication factor-A protein 1, single-stranded DNA-binding protein, p70, REPA1, RPA1, and HSSB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPA70 Antibody (NB100-2204)(6)

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPA1
Entrez
Uniprot