RP105/CD180 Antibody

Western Blot: RP105/CD180 Antibody [NBP2-20204] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: RP105/CD180 Antibody [NBP2-20204] - Mahlarvu xenograft, using RP105 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Flow Cytometry: RP105/CD180 Antibody [NBP2-20204] - Flow cytometry on Jurkat cells, staining with RP105 antibody at 1:100 dilution (blue) or rabbit IgG (black).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RP105/CD180 Antibody Summary

• Description: Novus Biologicals Rabbit RP105/CD180 Antibody (NBP2-20204) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RP105/CD180. The exact sequence is proprietary.
• Localization: Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD180
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: ICC/IF Assay dependent
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RP105/CD180 Antibody

• CD180 antigenMGC126233
• CD180 molecule
• CD180
• Ly64
• LY64MGC126234
• Ly78
• Lymphocyte antigen 64
• lymphocyte antigen-64, radioprotective, 105kDa
• Radioprotective 105 kDa protein
• RP105
• RP105lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD

Background

CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CD180
• Uniprot:
  • Q99467(Human)