RP105/CD180 Antibody

Images

 
Western Blot: RP105/CD180 Antibody [NBP2-20204] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: RP105/CD180 Antibody [NBP2-20204] - Mahlarvu xenograft, using RP105 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Flow Cytometry: RP105/CD180 Antibody [NBP2-20204] - Flow cytometry on Jurkat cells, staining with RP105 antibody at 1:100 dilution (blue) or rabbit IgG (black).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RP105/CD180 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RP105. The exact sequence is proprietary.
Localization
Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD180
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Application Notes
ICC/IF Assay dependent
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for RP105/CD180 Antibody

  • CD180 antigenMGC126233
  • CD180 molecule
  • CD180
  • Ly64
  • LY64MGC126234
  • Ly78
  • Lymphocyte antigen 64
  • lymphocyte antigen-64, radioprotective, 105kDa
  • Radioprotective 105 kDa protein
  • RP105
  • RP105lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD

Background

CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD180
Uniprot