RNA Polymerase II/POLR2A [p Thr4] Antibody

Images

 
Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Flag-tagged Rpb1 was IP'd with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat ...read more
Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Analysis of RNA pol II CTD pT4 in A. DT40 cell lysate and B. HeLa cell lysate.
Dot Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Dot blot analysis of RNA pol II CTD pT4 using phosphorylated and non-phosphorylated peptides.

Product Details

Summary
Reactivity Hu, Ch, Dr, YeSpecies Glossary
Applications WB, DB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

RNA Polymerase II/POLR2A [p Thr4] Antibody Summary

Immunogen
A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]
Modification
p Thr4
Localization
Nucleus.
Specificity
This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Dot Blot 1:1000
Application Notes
This RNA polymerase II antibody is useful for dot blot and Western blot.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49546 in the following applications:

Publications
Read Publications using
NBP1-49546 in the following applications:

  • WB
    3 publications

Reactivity Notes

Human, chicken, Drosophila and yeast.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNA Polymerase II/POLR2A [p Thr4] Antibody

  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase II subunit RPB1
  • DNA-directed RNA polymerase III largest subunit
  • EC 2.7.7.48
  • EC 2.7.7.6
  • hRPB220
  • hsRPB1
  • MGC75453
  • POLR2
  • POLR2A
  • POLRA
  • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • RNA Pol 2
  • RNA Polymerase 2
  • RNA polymerase II subunit B1
  • RNA Polymerase II
  • RNA-directed RNA polymerase II subunit RPB1
  • RNAPII
  • RPB1
  • RPBh1
  • RpIILS
  • RPO2
  • RPOL2

Background

RNA polymerase II (RNAPII) is a 12-subunit enzyme which synthesizes all mRNAs as well as several non-coding RNAs in eukaryotic cells thereby playing a central role in cellular functions. RNAPII is a DNA-dependent RNA polymerase enzyme which catalyzes the transcription of DNA into RNA employing four ribonucleoside triphosphates as substrates. The activity of RNAPII transcription machinery is regulated by DNA-binding transcriptional regulators in a gene/cell type-specific manner, whereas general transcription factors act as RNAPII accessory proteins required for the transcription of most class II genes, and the co-activators/co-repressors serve as bridges between DNA-bound factors and the RNAPII machinery, some affecting the organization and/or chemical modification of the chromatin template of RNAPII. RNA polymerase II subunit B1 (POLR2A) is the largest and the catalytic component of RNAPII which forms the polymerase active center together with the second largest subunit and is the central component of the basal RNAPII transcription machinery. It is composed of mobile elements that move relative to each other and POLR2A is the part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNA Polymerase II/POLR2A Antibody (NBP1-49546)(3)

Review for RNA Polymerase II/POLR2A Antibody (NBP1-49546) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-49546:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot RNA Polymerase II/POLR2A NBP1-49546
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reviewed by:
Anonymous
WB Human 08/26/2014
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Summary

ApplicationWestern Blot
Sample TestedHEK293T cells
SpeciesHuman
CommentsThe increase in phosphorylation on RNA Pol II is not marked, probably because of lesser HU used. But the antibody works good.

Blocking

Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:2000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Descriptionanti-rabbit IgG HRP conjugate (Jackson Labs)
Secondary Manufacturer Cat#111-005-045
Secondary Concentration1:20,000

Details

Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 5 sec exposure

Comments

CommentsThe increase in phosphorylation on RNA Pol II is not marked, probably because of lesser HU used. But the antibody works good.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RNA Polymerase II/POLR2A Antibody (NBP1-49546) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49546AF405
Alexa Fluor 488 Labeled NBP1-49546AF488
Alexa Fluor 647 Labeled NBP1-49546AF647
Alexa Fluor 700 Labeled NBP1-49546AF700
Biotin Labeled NBP1-49546B
DyLight 405 Labeled NBP1-49546V
DyLight 488 Labeled NBP1-49546G
DyLight 550 Labeled NBP1-49546R
DyLight 650 Labeled NBP1-49546C
HRP Labeled NBP1-49546H

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
08/26/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol POLR2A
Entrez
Uniprot