Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Western Blot analysis of RNA Pol II (pThr 4) in 293T cells. Image submitted via verified customer review.
Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Analysis of RNA pol II CTD pT4 in A. DT40 cell lysate and B. HeLa cell lysate.
Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Flag-tagged Rpb1 was IP'd with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat ...read more
Dot Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Dot blot analysis of RNA pol II CTD pT4 using phosphorylated and non-phosphorylated peptides.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for RNA Polymerase II/POLR2A [p Thr4] Antibody
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
DNA-directed RNA polymerase II subunit A
DNA-directed RNA polymerase II subunit RPB1
DNA-directed RNA polymerase III largest subunit
polymerase (RNA) II (DNA directed) polypeptide A (220kD)
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
RNA Pol 2
RNA Polymerase 2
RNA polymerase II subunit B1
RNA Polymerase II
RNA-directed RNA polymerase II subunit RPB1
RNA polymerase II (RNAPII) is a 12-subunit enzyme which synthesizes all mRNAs as well as several non-coding RNAs in eukaryotic cells thereby playing a central role in cellular functions. RNAPII is a DNA-dependent RNA polymerase enzyme which catalyzes the transcription of DNA into RNA employing four ribonucleoside triphosphates as substrates. The activity of RNAPII transcription machinery is regulated by DNA-binding transcriptional regulators in a gene/cell type-specific manner, whereas general transcription factors act as RNAPII accessory proteins required for the transcription of most class II genes, and the co-activators/co-repressors serve as bridges between DNA-bound factors and the RNAPII machinery, some affecting the organization and/or chemical modification of the chromatin template of RNAPII. RNA polymerase II subunit B1 (POLR2A) is the largest and the catalytic component of RNAPII which forms the polymerase active center together with the second largest subunit and is the central component of the basal RNAPII transcription machinery. It is composed of mobile elements that move relative to each other and POLR2A is the part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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