RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free

Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Western Blot analysis of RNA Pol II (pThr 4) in 293T cells. Image submitted via verified customer review.

Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Analysis of RNA pol II CTD pT4 in A. DT40 cell lysate and B. HeLa cell lysate.

Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Flag-tagged Rpb1 was IP'd with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat CTD. T4V: Rpb1 with 30 YSPVSPS repeat CTD.

Dot Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Dot blot analysis of RNA pol II CTD pT4 using phosphorylated and non-phosphorylated peptides.

• Reactivity: Hu, Ch, Dr, Ye
• Applications: WB, DB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.1 mg/ml

RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free (NBP1-49546) is a polyclonal antibody validated for use in WB. Anti-RNA Polymerase II/POLR2A Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]
• Modification: p Thr4
• Localization: Nucleus.
• Specificity: This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:1000
  • Western Blot 1:1000
• Application Notes: This RNA polymerase II antibody is useful for dot blot and Western blot.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free

• DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
• DNA-directed RNA polymerase II subunit A
• DNA-directed RNA polymerase II subunit RPB1
• DNA-directed RNA polymerase III largest subunit
• EC 2.7.7.48
• EC 2.7.7.6
• hRPB220
• hsRPB1
• MGC75453
• POLR2
• POLR2A
• POLRA
• polymerase (RNA) II (DNA directed) polypeptide A (220kD)
• polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• RNA Pol 2
• RNA Polymerase 2
• RNA polymerase II subunit B1
• RNA Polymerase II
• RNA-directed RNA polymerase II subunit RPB1
• RNAPII
• RPB1
• RPBh1
• RpIILS
• RPO2
• RPOL2

Background

RNA polymerase II (RNAPII) is a 12-subunit enzyme which synthesizes all mRNAs as well as several non-coding RNAs in eukaryotic cells thereby playing a central role in cellular functions. RNAPII is a DNA-dependent RNA polymerase enzyme which catalyzes the transcription of DNA into RNA employing four ribonucleoside triphosphates as substrates. The activity of RNAPII transcription machinery is regulated by DNA-binding transcriptional regulators in a gene/cell type-specific manner, whereas general transcription factors act as RNAPII accessory proteins required for the transcription of most class II genes, and the co-activators/co-repressors serve as bridges between DNA-bound factors and the RNAPII machinery, some affecting the organization and/or chemical modification of the chromatin template of RNAPII. RNA polymerase II subunit B1 (POLR2A) is the largest and the catalytic component of RNAPII which forms the polymerase active center together with the second largest subunit and is the central component of the basal RNAPII transcription machinery. It is composed of mobile elements that move relative to each other and POLR2A is the part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNA Polymerase II/POLR2A Antibody (NBP1-49546)(4)

Publications using NBP1-49546

• Kamieniarz-Gdula K, Gdula MR, Panser K et al. Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination Mol. Cell 2019-02-21 [PMID: 30819644] (WB, Human)
• Allepuz-Fuster P, Martinez-Fernandez V, Garrido-Godino Ai et al. Rpb4/7 facilitates RnA polymerase II CTD dephosphorylation. Nucleic Acids Res. 2014-11-21 [PMID: 25416796] (WB, Yeast)
• Hsin JP, Xiang K, Manley JL. Function and control of RNA polymerase II CTD phosphorylation in vertebrate transcription and RNA processing. Mol. Cell. Biol. 2014-04-21 [PMID: 24752900] (WB, Human)
• Hsin JP, Sheth A, Manley JL. RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3' end processing. Science;334(6056):683-6. 2011-11-04 [PMID: 22053051] (WB, Human, Drosophila, Yeast)

Review for RNA Polymerase II/POLR2A Antibody (NBP1-49546) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/26/2014

Summary

• Application: Western Blot
• Sample Tested: HEK293T cells
• Species: Human

Bioinformatics

• Gene Symbol: POLR2A
• Entrez:
  • 5430(Human)
• Uniprot:
  • P24928(Human)