USP7 Antibody

Images

 
Western Blot: USP7 Antibody [NB100-513] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP7 antibodyused for WB at 0.1 ...read more
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-USP7 (left) and (right) used at a dilution of 1:1,000 (1ug/ml). ...read more
Independent Antibodies: Western Blot: USP7 Antibody [NB100-513] - Detection of Human and Mouse USP7 by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug ...read more
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - FFPE sections of human ovarian carcinoma. Antibodies: Affinity purified rabbit anti-USP7 (left) and (right) used at a dilution of 1:1,000 (1ug/ml). Detection: ...read more
Independent Antibodies: Immunoprecipitation: USP7 Antibody [NB100-513] - Detection of human USP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

USP7 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Ubiquitin Specific Peptidase 7 using the numbering given in entry NP_003461.1 (GeneID 7874).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1-4 ug/ml of lysate
Application Notes
Many tissues will not require epitope retrieval. However to boost weak staining, epitope retrieval with citrate buffer at pH 6.0 is recommended for FFPE tissue sections. Use in ICC/IF reported by customer review.
Theoretical MW
128 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
USP7 Overexpression Lysate
USP7 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-513 in the following applications:

Publications
Read Publications using
NB100-513 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP7 Antibody

  • Deubiquitinating enzyme 7
  • EC 3.1.2.15
  • EC 3.4.19.12
  • HAUSP
  • HAUSPTEF1
  • Herpes virus-associated ubiquitin-specific protease
  • Herpesvirus-associated ubiquitin-specific protease
  • TEF1
  • ubiquitin carboxyl-terminal hydrolase 7
  • ubiquitin specific peptidase 7 (herpes virus-associated)
  • ubiquitin specific protease 7 (herpes virus-associated)
  • Ubiquitin thioesterase 7
  • Ubiquitin-specific-processing protease 7
  • USP7

Background

USP7 (also called HAUSP) is a de-ubiquitinating enzyme recently identified as a key regulator of the p53-mdm2 pathway, which stabilizes both p53 and mdm2. The Epstein-Barr nuclear antigen 1 protein of Epstein-Barr virus binds with high affinity to USP7 and disrupts the USP7-p53 interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP7 Antibody (NB100-513)(25)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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(1)
WB
(1)
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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB100-513 Applications Species
Cui H, Guo M, Xu D et al. The stress-responsive gene ATF3 regulates the histone acetyltransferase Tip60 Nat Commun 2015 Apr 13 [PMID: 25865756] (WB, IP, Human) WB, IP Human
de Lange J, Teunisse AF, Vries MV et al. High levels of Hdmx promote cell growth in a subset of uveal melanomas. Am J Cancer Res 2012 [PMID: 22957303]
Fei J, Chen J. KIAA1530 protein is recruited by Cockayne syndrome complementation group protein A (CSA) to participate in transcription-coupled repair (TCR). J Biol Chem 2012 Oct [PMID: 22902626]
Cano F, Bye H, Duncan LM et al. The RNA-binding E3 ubiquitin ligase MEX-3C links ubiquitination with MHC-I mRNA degradation. EMBO J 2012 Aug [PMID: 22863774]
Dekker P, Gunn D, McBryan T et al. Microarray-based identification of age-dependent differences in gene expression of human dermal fibroblasts. Mech Ageing Dev 2012 Jul [PMID: 22721680]
Lenos K, Grawenda AM, Lodder K et al. Alternate splicing of the p53 inhibitor HDMX offers a superior prognostic biomarker than p53 mutation in human cancer. Cancer Res 2012 Aug [PMID: 22700878]
Schwertman P, Lagarou A, Dekkers DH et al. UV-sensitive syndrome protein UVSSA recruits USP7 to regulate transcription-coupled repair. Nat Genet 2012 May [PMID: 22466611]
Felle M, Joppien S, Nemeth A et al. The USP7/Dnmt1 complex stimulates the DNA methylation activity of Dnmt1 and regulates the stability of UHRF1. Nucleic Acids Res 2011 Oct [PMID: 21745816]
Gunaratne J, Goh MX, Swa HL et al. Protein interactions of phosphatase and tensin homologue (PTEN) and its cancer-associated G20E mutant compared by using stable isotope labeling by amino acids in cell culture-based parallel affinity purification. J Biol Chem 2011 May [PMID: 21454687]
McFarlane S, Preston CM. Human cytomegalovirus immediate early gene expression in the osteosarcoma line U2OS is repressed by the cell protein ATRX. Virus Res 2011 Apr [PMID: 21310198]
Show All 25 Publications.

Review for USP7 Antibody (NB100-513) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-513:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence USP7 NB100-513
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reviewed by:
Anonymous
IF Human 05/21/2019
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Summary

ApplicationImmunofluorescence
Sample TestedHEK293T cells
SpeciesHuman
LotA8

Comments

CommentsHEK293T cells
1:100 dilution
incubation overnight 4°C

Product General Protocols

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FAQs for USP7 Antibody (NB100-513) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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1
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Anonymous
05/21/2019
Application: IF
Species: Human

Bioinformatics

Gene Symbol USP7
Entrez
Uniprot
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