USP7 Antibody

Images

 
Western Blot: USP7 Antibody [NB100-513] - Detection of Human and Mouse USP7 by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP ...read more
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - FFPE section of human stomach carcinoma.
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Human stomach adenocarcinoma. Antibody used at a dilution of 1:500. Detection: DAB
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Mouse squamous cell carcinoma. Antibody used at a dilution of 1:100. Detection: DAB.
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - FFPE section of human colon adenocarcinoma. Antibody used at a dilution of 1:100. Detection: Green-fluorescent Anti-rabbit IgG FITC conjugated used at a ...read more
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - FFPE section of human breast adenocarcinoma. Antibody used at a dilution of 1:100. Detection: Green-fluorescent Anti-rabbit IgG FITC conjugated used at a ...read more
Proximity Ligation Assay: USP7 Antibody [NB100-513] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human USP7 (NB100-513). Image merged from DAPI (2ms), FITC/Green ...read more
Proximity Ligation Assay: USP7 Antibody [NB100-513] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human USP7 (NB100-513). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: USP7 Antibody [NB100-513] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HdmX (NBP2-22228) and rabbit anti-human USP7 (NB100-513). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: USP7 Antibody [NB100-513] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM4 (NB100-250) and rabbit anti-human USP7 (NB100-513). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: USP7 Antibody [NB100-513] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM6 (NB100-251) and rabbit anti-human USP7 (NB100-513). Image merged from DAPI (2ms) and Texas Red ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

USP7 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Ubiquitin Specific Peptidase 7 using the numbering given in entry NP_003461.1 (GeneID 7874).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Many tissues will not require epitope retrieval. However to boost weak staining epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
128 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
USP7 Lysate (NBL1-17671)
Publications
Read Publications using
NB100-513 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP7 Antibody

  • Deubiquitinating enzyme 7
  • EC 3.1.2.15
  • EC 3.4.19.12
  • HAUSP
  • HAUSPTEF1
  • Herpes virus-associated ubiquitin-specific protease
  • Herpesvirus-associated ubiquitin-specific protease
  • TEF1
  • ubiquitin carboxyl-terminal hydrolase 7
  • ubiquitin specific peptidase 7 (herpes virus-associated)
  • ubiquitin specific protease 7 (herpes virus-associated)
  • Ubiquitin thioesterase 7
  • Ubiquitin-specific-processing protease 7
  • USP7

Background

USP7 (also called HAUSP) is a de-ubiquitinating enzyme recently identified as a key regulator of the p53-mdm2 pathway, which stabilizes both p53 and mdm2. The Epstein-Barr nuclear antigen 1 protein of Epstein-Barr virus binds with high affinity to USP7 and disrupts the USP7-p53 interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP7 Antibody (NB100-513)(25)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(1)
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Human
(1)
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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB100-513 Applications Species
Cui H, Guo M, Xu D et al. The stress-responsive gene ATF3 regulates the histone acetyltransferase Tip60 Nat Commun 2015 Apr 13 [PMID: 25865756] (WB, IP, Human) WB, IP Human
de Lange J, Teunisse AF, Vries MV et al. High levels of Hdmx promote cell growth in a subset of uveal melanomas. Am J Cancer Res 2012 [PMID: 22957303]
Fei J, Chen J. KIAA1530 protein is recruited by Cockayne syndrome complementation group protein A (CSA) to participate in transcription-coupled repair (TCR). J Biol Chem 2012 Oct [PMID: 22902626]
Cano F, Bye H, Duncan LM et al. The RNA-binding E3 ubiquitin ligase MEX-3C links ubiquitination with MHC-I mRNA degradation. EMBO J 2012 Aug [PMID: 22863774]
Dekker P, Gunn D, McBryan T et al. Microarray-based identification of age-dependent differences in gene expression of human dermal fibroblasts. Mech Ageing Dev 2012 Jul [PMID: 22721680]
Lenos K, Grawenda AM, Lodder K et al. Alternate splicing of the p53 inhibitor HDMX offers a superior prognostic biomarker than p53 mutation in human cancer. Cancer Res 2012 Aug [PMID: 22700878]
Schwertman P, Lagarou A, Dekkers DH et al. UV-sensitive syndrome protein UVSSA recruits USP7 to regulate transcription-coupled repair. Nat Genet 2012 May [PMID: 22466611]
Felle M, Joppien S, Nemeth A et al. The USP7/Dnmt1 complex stimulates the DNA methylation activity of Dnmt1 and regulates the stability of UHRF1. Nucleic Acids Res 2011 Oct [PMID: 21745816]
Gunaratne J, Goh MX, Swa HL et al. Protein interactions of phosphatase and tensin homologue (PTEN) and its cancer-associated G20E mutant compared by using stable isotope labeling by amino acids in cell culture-based parallel affinity purification. J Biol Chem 2011 May [PMID: 21454687]
McFarlane S, Preston CM. Human cytomegalovirus immediate early gene expression in the osteosarcoma line U2OS is repressed by the cell protein ATRX. Virus Res 2011 Apr [PMID: 21310198]
Show All 25 Publications.

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol USP7
Entrez
Uniprot