| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB200-598SS | |||
| Azide and BSA Free | NBP2-80928 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49546SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16906-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6160-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50274-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50243-0.025ml | |||
![Western Blot: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - Whole cell protein from HeLa (lane 1), MCF7 (lane 2), NIH-3T3 (lane 3) and Cos7 (lane 4) were separated by SDS-PAGE on a 6% gel, transferred to PVDF and probed with 2 ug/mL NB200-598. RNA Pol II reactivity (arrowhead) is observed in HeLa, MCF and 3T3 cells.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-4H8-Western-Blot-NB200-598-img0010.jpg "Western Blot: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - Whole cell protein from HeLa (lane 1), MCF7 (lane 2), NIH-3T3 (lane 3) and Cos7 (lane 4) were separated by SDS-PAGE on a 6% gel, transferred to PVDF and probed with 2 ug/mL NB200-598. RNA Pol II reactivity (arrowhead) is observed in HeLa, MCF and 3T3 cells.")
![Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - RNA Polymerase II/POLR2A antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-4H8-Immunocytochemistry-Immunofluorescence-NB200-598-img0011.jpg)
![Flow Cytometry: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - An intracellular stain was performed on HeLa cells with RNA Polymerase II/POLR2A (4H8) antibody NB200-598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-4H8-Flow-Cytometry-NB200-598-img0013.jpg)
![Western Blot: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - Analysis of MCF7 cell lysata (A) using RNA polymerase II antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-4H8-Western-Blot-NB200-598-img0009.jpg)
![Flow (Intracellular): RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - An intracellular stain was performed on Daudi cells with RNA Polymerase II/POLR2A (4H8) antibody NB200-598 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-4H8-Flow-Intracellular-NB200-598-img0012.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-PhosphoRNA Polymerase II (S2) used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0029.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Hela whole cell lysate (50 ug each lane) loaded and probed by anti-RNA polymerase II Antibody. NB100-1805 was pre-incubated without peptide (-), with non-phosphorylated peptide (NP), with phosphorylated S2 peptide, or with phosphorylated S5 peptide.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0021.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.08 ug/ml.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0022.jpg)
![Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Analysis of POLR2A in MCF-7 xenograft using anti-POLR2A [p Ser2] antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0023.jpg)
![Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Human ovarian carcinoma. Antibody: Affinity purified rabbit antiPhospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0028.jpg)
![RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. immunoprecipitates were either phosphatase treated (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 used for IP at 5 ug per reaction and another Affinity purified rabbit anti-RNA Polymerase II antibody used for IP at 5 ug per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S2) and total RNA Polymerase II, NB100-1805 was used at 0.1 ug/ml (upper panel) and the other antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 75 seconds (lower).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-NB100-1805-img0030.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Western Blot analysis of RNA Pol II (pThr 4) in 293T cells. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Thr4]-Antibody-Western-Blot-NBP1-49546-img0008.jpg "Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Western Blot analysis of RNA Pol II (pThr 4) in 293T cells. Image submitted via verified customer review.")
![Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Analysis of RNA pol II CTD pT4 in A. DT40 cell lysate and B. HeLa cell lysate.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Thr4]-Antibody-Western-Blot-NBP1-49546-img0006.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Flag-tagged Rpb1 was IP'd with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat CTD. T4V: Rpb1 with 30 YSPVSPS repeat CTD.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Thr4]-Antibody-Western-Blot-NBP1-49546-img0007.jpg)
![Dot Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Dot blot analysis of RNA pol II CTD pT4 using phosphorylated and non-phosphorylated peptides.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Thr4]-Antibody-Dot-Blot-NBP1-49546-img0005.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Western-Blot-NBP2-59215-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S2p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Chromatin-Immunoprecipitation-NBP2-59215-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Western-Blot-NBP2-59215-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Cross reactivity of the antibody directed against Pol IIS2p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS2p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S2 phosphorylation.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-ELISA-NBP2-59215-img0003.jpg)
![Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Immunofluorescence-NBP2-59215-img0005.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Western-Blot-NBP2-59219-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S5p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter and the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Chromatin-Immunoprecipitation-NBP2-59219-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Western-Blot-NBP2-59219-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Cross reactivity of the antibody directed against Pol IIS5p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS5p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S5 phosphorylation.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-ELISA-NBP2-59219-img0003.jpg)
![Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Immunofluorescence-NBP2-59219-img0005.jpg)
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A Antibody [NBP2-32080] - ChIP-qPCR analysis of RNA polymerase II binding at pause site of the Jun B gene. Chromatin isolated from one 15 cm plate of HeLa cells was immunoprecipitated with 8.0 ug of anti-RNA polymerase II antibodies and PCR amplified.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-Chromatin-Immunoprecipitation-NBP2-32080-img0002.jpg "Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A Antibody [NBP2-32080] - ChIP-qPCR analysis of RNA polymerase II binding at pause site of the Jun B gene. Chromatin isolated from one 15 cm plate of HeLa cells was immunoprecipitated with 8.0 ug of anti-RNA polymerase II antibodies and PCR amplified.")
![Immunoprecipitation: Rabbit Polyclonal RNA Polymerase II/POLR2A Antibody [NBP2-32080] - Detection of human RNA Polymerase II by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody (NBP2-32080 lot 2) used for IP at 6 ug per reaction. RNA Polymerase II was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of RNA Polymerase II antibody. For blotting immunoprecipitated RNA Polymerase II, the second antibody was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/antibody/nbp2-32080_rabbit-polyclonal-rna-polymerase-ii-polr2a-antibody-immunoprecipitation-1552024102422..png)
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Western blot analysis of extracts of various cell lines, using RNA Polymerase II/POLR2A Rabbit mAb (NBP3-16910) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours or treated by nocodazole (50 ng/mL) at 37C for 20 hours or treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-10C7U2-Western-Blot-NBP3-16910-img0001.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Western blot analysis of extracts of various cell lines, using RNA Polymerase II/POLR2A Rabbit mAb (NBP3-16910) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours or treated by nocodazole (50 ng/mL) at 37C for 20 hours or treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-522025124253.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of A-549 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511374134.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-5220251215456.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511383749.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-5220251138375.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511411428.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of PC-12 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511365122.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511411458.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of NIH/3T3 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511374150.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of HeLa cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511374149.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511402793.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202511392267.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-5220251140272.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517304996.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517333825.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of PC-12 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517361292.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517303121.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517352723.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517304937.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of NIH/3T3 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517352717.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517344260.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-5220251736128.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517352732.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517311066.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of HeLa cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-5220251736370.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of A-549 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16910_rabbit-rna-polymerase-ii-polr2a-p-ser-5-mab-10c7u2-52202517304990.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser7] Antibody [NBP3-16906] - Western blot analysis of extracts of MCF7 cells, using RNA Polymerase II/POLR2A antibody (NBP3-16906) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser7]-Antibody-Western-Blot-NBP3-16906-img0001.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser7] Antibody [NBP3-16906] - Western blot analysis of extracts of MCF7 cells, using RNA Polymerase II/POLR2A antibody (NBP3-16906) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202511361258.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202511365198.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202511374167.jpg)
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa cells, using RNA Polymerase II/POLR2A Rabbit pAb and rabbit IgG . The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202511402732.jpg)
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [RNA Polymerase II/POLR2A] - Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa cells, using RNA Polymerase II/POLR2A Rabbit pAb and rabbit IgG . The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202517304957.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-5220251736123.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-5220251733384.jpg)
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202517304987.jpg)
![Western Blot: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-Western-Blot-NBP2-59213-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-Immunofluorescence-NBP2-59213-img0005.jpg)
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - ChIP assays were performed using human HeLa cells, the antibody against Pol II and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter and the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-Chromatin-Immunoprecipitation-NBP2-59213-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-Western-Blot-NBP2-59213-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - Cross reactivity of the antibody directed against Pol II. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol II. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows that the antibody recognizes the unphosphorylated Pol II as well as most phosphorylated forms.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-ELISA-NBP2-59213-img0003.jpg)
![Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 4H8) [NBP2-50274] - Monoclonal antibody 4H8. RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNA polymerase II (RNAP II) contains a highly conserved heptapeptide repeat, YSPTSPS. Monoclonal antibody 4H8 was obtained using synthetic peptide and specifically recognises the C-terminal domain of RNAP II.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-CTD-4H8-Western-Blot-NBP2-50274-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 4H8) [NBP2-50274] - Monoclonal antibody 4H8. RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNA polymerase II (RNAP II) contains a highly conserved heptapeptide repeat, YSPTSPS. Monoclonal antibody 4H8 was obtained using synthetic peptide and specifically recognises the C-terminal domain of RNAP II.")
![Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 8A7) [NBP2-50243] - RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNAP II is reversibly phosphorylated on the highly conserved heptapeptide repeat, YSPTSPS. The major physiological phosphorylation sites are Ser-2 and Ser-5. Monoclonal antibody 8A7 was obtained using synthetic phosphopeptides and specifically recognises the Ser-5 phosphorylated form of RNAP II.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-CTD-8A7-Western-Blot-NBP2-50243-img0001.jpg "Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 8A7) [NBP2-50243] - RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNAP II is reversibly phosphorylated on the highly conserved heptapeptide repeat, YSPTSPS. The major physiological phosphorylation sites are Ser-2 and Ser-5. Monoclonal antibody 8A7 was obtained using synthetic phosphopeptides and specifically recognises the Ser-5 phosphorylated form of RNAP II.")