POLR2C Antibody - BSA Free

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP1-80818] - Staining of human cerebral cortex, colon, kidney and testis using Anti-POLR2C antibody NBP1-80818 (A) shows similar protein distribution across tissues to independent antibody NBP1-80817 (B).

Western Blot: POLR2C Antibody [NBP1-80818] - Analysis using Anti-POLR2C antibody NBP1-80818 (A) shows similar pattern to independent antibody NBP1-80817 (B).

Immunocytochemistry/Immunofluorescence: POLR2C Antibody [NBP1-80818] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP1-80818] - Staining of human testis.

Western Blot: POLR2C Antibody [NBP1-80818] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP1-80818] - Staining of human kidney.

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP1-80818] - Staining of human colon.

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP1-80818] - Staining of human cerebral cortex.

ChIP-Exo-Seq composite graph for Anti-POLR2C (NBP1-80818) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POLR2C Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  POLR2C Protein (NBP1-80818PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for POLR2C Antibody - BSA Free

• DNA-directed RNA polymerase II 33 kDa polypeptide
• DNA-directed RNA polymerase II subunit C
• DNA-directed RNA polymerase II subunit RPB3
• hRPB33
• hsRPB3
• polymerase (RNA) II (DNA directed) polypeptide C (33kD)
• polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
• RNA polymerase II subunit 3
• RNA polymerase II subunit B3
• RPB3
• RPB31
• RPB33

Background

The POLR2C gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POLR2C Antibody (NBP1-80818)(1)

Publication using NBP1-80818

• E G, Sun B, Liu B et al. Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2 Brain research bulletin 2022-11-08 [PMID: 36334804] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000

Bioinformatics

• Gene Symbol: POLR2C
• Entrez:
  • 5432(Human)
• Uniprot:
  • P19387(Human)