RNA Polymerase II/POLR2A [p Thr4] Antibody [DyLight 488]

• Reactivity: Hu, Ch, Dr, Ye
• Applications: WB, DB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

RNA Polymerase II/POLR2A [p Thr4] Antibody [DyLight 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]
• Modification: p Thr4
• Localization: Nucleus.
• Specificity: This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP1-49546. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for RNA Polymerase II/POLR2A [p Thr4] Antibody [DyLight 488]

• DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
• DNA-directed RNA polymerase II subunit A
• DNA-directed RNA polymerase II subunit RPB1
• DNA-directed RNA polymerase III largest subunit
• EC 2.7.7.48
• EC 2.7.7.6
• hRPB220
• hsRPB1
• MGC75453
• POLR2
• POLR2A
• POLRA
• polymerase (RNA) II (DNA directed) polypeptide A (220kD)
• polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• RNA Pol 2
• RNA Polymerase 2
• RNA polymerase II subunit B1
• RNA Polymerase II
• RNA-directed RNA polymerase II subunit RPB1
• RNAPII
• RPB1
• RPBh1
• RpIILS
• RPO2
• RPOL2

Background

RNA polymerase II catalyzes DNA transcription into RNA, and is composed of 12 subunits. The largest of these subunits is B1 (RPB1, POLR2A), which contains the DNA binding domain and the enzymatic subunit. RPB1 contains multiple heptapeptide repeats in the CTD arranged in the sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7 (YSPTSPS). Modifications of this sequence, such as phosphorylation, alter polymerase activity. Serines 2, 5, and 7 are known to become phosphorylated to lead the complex through the transcription cycle, as well as functions in polyadenylation, mRNA capping, and snRNA-processing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2A