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RPB2 Antibody

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Western Blot: RPB2 Antibody [NBP2-53092] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: RPB2 Antibody [NBP2-53092] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RPB2 Antibody [NBP2-53092] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: RPB2 Antibody [NBP2-53092] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: RPB2 Antibody [NBP2-53092] - Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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RPB2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for RPB2 Antibody

  • DNA-directed RNA polymerase II subunit RPB2
  • EC 2.7.7
  • EC 2.7.7.6
  • hRPB140
  • hsRPB2
  • POL2RB
  • polymerase (RNA) II (DNA directed) polypeptide B (140kD)
  • polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
  • RNA polymerase II subunit 2
  • RNA polymerase II subunit B2
  • RNA polymerase II subunit2, RNA polymerase II second largest subunit

Background

RBP2 is an abundant protein present in the small intestinal epithelium. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. RBP2 may also modulate the supply of retinoic acid to the nuclei of endometrial cells during the menstrual cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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RNA Polymerase
II/POLR2A Antibody (4
NB200-598
Western Blot: RNA polymerase II Antibody (4H8) [NB200-598] - Whole cell protein from HeLa (lane 1), MCF7 (lane 2), NIH-3T3 (lane 3) and Cos7 (lane 4) were separated by SDS-PAGE on a 6% gel, transferred to PVDF and probed with 2 ug/ml NB200-598. RNA Pol II reactivity (arrowhead) is observed in HeLa, MCF and 3T3 cells.
Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - RNA Polymerase II/POLR2A antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Flow (Intracellular): RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - An intracellular stain was performed on Daudi cells with RNA Polymerase II/POLR2A (4H8) antibody NB200-598 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
(1 Review)
(6 Publications)
Species: Hu, Mu, Ye, Pm(-)
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IP, CyTOF-ready, Flow-IC
Plexin A1 Antibody
[Unconjugated]
AF4309
Western blot shows lysates of mouse embryonic heart tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Plexin A1 was detected in immersion fixed frozen sections of embryonic mouse spinal cord using Mouse Plexin A1 Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Plexin A1 was detected in immersion fixed immature human dendritic cells using Goat Anti-Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Species: Hu, Mu
Applications: WB, IHC, ICC
RPL10 Antibody
NBP1-84037
Western Blot: RPL10 Antibody [NBP1-84037] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: RPL10 Antibody [NBP1-84037] - Staining in human fallopian tube and cerebral cortex tissues. Corresponding RPL10 RNA-seq data are presented for the same tissues.
Western Blot: RPL10 Antibody [NBP1-84037] - Analysis in human cell line HeLa.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NOV/CCN3 Antibody
AF1640
NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(2 Publications)
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ELISA(Sta)
TEF1 Antibody
(13112)
NBP2-43581
Western Blot: TEF1 Antibody (13112) [NBP2-43581] - Analysis of whole cell extracts and extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [13112] at a dilution of 1:500.
Immunocytochemistry/Immunofluorescence: TEF1 Antibody (13112) [NBP2-43581] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 antibody [GT13112] diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.
Immunoprecipitation: TEF1 Antibody (13112) [NBP2-43581] - Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 ug of TEF1 antibody [13112]. Western blot analysis was performed using TEF1 antibody [13112] EasyBlot anti-Mouse IgG was used as a secondary reagent.
Species: Hu
Applications: WB, ICC/IF, IP
USP7 Antibody
NB100-513
Western Blot: USP7 Antibody [NB100-513] - Detection of Human and Mouse USP7 by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP7 antibody NB100-513 (lot NB100-513-3) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. USP7 was also immunoprecipitated by a previous lot (lot NB100-513-2) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - FFPE section of human stomach carcinoma.
Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Human stomach adenocarcinoma. Antibody used at a dilution of 1:500. Detection: DAB
(25 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, PLA
POLR2C Antibody
NBP2-19885
Western Blot: POLR2C Antibody [NBP2-19885] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: POLR2C Antibody [NBP2-19885] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLR2C protein stained by POLR2C antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885] - Paraffin-embedded mouse liver. POLR2C antibody [N1C3-2] dilution: 1:500.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
POLR2E Antibody
(OTI3B5)
NBP2-00482
Western Blot: POLR2E Antibody (3B5) [NBP2-00482] Analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (3B5) [NBP2-00482] - Human tonsil.
Flow Cytometry: POLR2E Antibody (3B5) [NBP2-00482] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2E antibody, and then analyzed by flow cytometry.
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, IHC, IHC-P
POLR2J Antibody
NBP2-46733
Immunocytochemistry/Immunofluorescence: POLR2J Antibody [NBP2-46733] - Staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry: POLR2J Antibody [NBP2-46733] - Staining of human liver tissue.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CSB Antibody
NB100-61070
Immunoprecipitation: CSB Antibody [NB100-61070] - Detection of Human ERCC6 on HeLa whole cell lysate using NB100-61070. ERCC6 was also immunoprecipitated by another rabbit anti-ERCC6 antibody NB100-61068 and NB100-61069.
Species: Hu
Applications: WB (-), IP
LXR beta/NR1H2
Antibody
NB100-74457
Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.
Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-74457] - Analysis of LXR beta (green) showing positive staining in the nuclear envelope of C2C12 cells (right) compared with a negative control in the absence of primary antibody (left).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
RPB8 Antibody
NBP1-80816
Western Blot: RPB8 Antibody [NBP1-80816] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining in human thyroid gland and pancreas tissues using anti-POLR2H antibody. Corresponding POLR2H RNA-seq data are presented for the same tissues.
Western Blot: RPB8 Antibody [NBP1-80816] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
TFIIB Antibody
NBP2-34143
Western Blot: TFIIB Antibody [NBP2-34143] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-GTF2B antibody. Remaining relative intensity is presented.
Immunocytochemistry/Immunofluorescence: TFIIB Antibody [NBP2-34143] - Staining of human cell line HEK 293 shows localization to nucleus & nuclear bodies.
Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining in human gallbladder and pancreas tissues using anti-GTF2B antibody. Corresponding GTF2B RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
POLR2L Antibody
NB120-14254
Western Blot: POLR2L Antibody [NB120-14254] - Western blot detection of E coli-derived POLR2L fusion protein. IgY dilutions: 1:2000, goat anti-IgY Fc-HRP: 1:1000, colorimetric method for signal development. ( anti-POLR2L )
Species: Hu, Mu
Applications: WB, IHC
POLR2G Antibody
NBP2-47329
Western Blot: POLR2G Antibody [NBP2-47329] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.
Immunocytochemistry/Immunofluorescence: POLR2G Antibody [NBP2-47329] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry: POLR2G Antibody [NBP2-47329] - Staining of human thyroid gland shows moderate nuclear positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Calmodulin 2
Knockout HeLa Cell Ly
NBP2-66296
Calmodulin 2 Knockout HeLa Cell Lysate
Species: Hu
Calmodulin
Antibody (2D1)
NB120-2860
Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.
Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.
Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, ICC, IF
Calmodulin 3
Antibody
NBP1-98474
Western Blot: Calmodulin 3 Antibody [NBP1-98474] - Human Jurkat, Antibody Dilution: 1.0 ug/ml CALM3 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.
Western Blot: Calmodulin 3 Antibody [NBP1-98474] - Titration: 1.0 ug/ml Positive Control: Hela Whole Cell.
Western Blot: Calmodulin 3 Antibody [NBP1-98474] - Human 721_B, Antibody Dilution: 1.0 ug/ml CALM3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.
Species: Hu
Applications: WB
RPB9 Antibody
NBP1-92344
Western Blot: RPB9 Antibody [NBP1-92344] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: RPB9 Antibody [NBP1-92344] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RPB9 Antibody [NBP1-92344] - Staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
RPB2 Antibody
NBP2-53092
RPB2 Antibody
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol POLR2B