RPB2 Antibody

Western Blot: RPB2 Antibody [NBP2-53092] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: RPB2 Antibody [NBP2-53092] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: RPB2 Antibody [NBP2-53092] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Western Blot: RPB2 Antibody [NBP2-53092] - Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RPB2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for RPB2 Antibody

• DNA-directed RNA polymerase II subunit RPB2
• EC 2.7.7
• EC 2.7.7.6
• hRPB140
• hsRPB2
• POL2RB
• polymerase (RNA) II (DNA directed) polypeptide B (140kD)
• polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
• RNA polymerase II subunit 2
• RNA polymerase II subunit B2
• RNA polymerase II subunit2, RNA polymerase II second largest subunit

Background

RBP2 is an abundant protein present in the small intestinal epithelium. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. RBP2 may also modulate the supply of retinoic acid to the nuclei of endometrial cells during the menstrual cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: POLR2B