POLR2E Antibody (OTI3B5)

Images

 
Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - POLR2A is destabilized by inhibition of oncogenic BRAFV600E signaling. Representative immunoblot of A375-P cells treated with PLX4720 or DMSO control for 16 hours, ...read more
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Human tonsil.
Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2E antibody, and then ...read more
Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - Analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human breast tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody.
Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - Analysis of Jurkat cells, using anti-POLR2E antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI3B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.9 mg/ml

Order Details

POLR2E Antibody (OTI3B5) Summary

Immunogen
Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POLR2E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-00482 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.9 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POLR2E Antibody (OTI3B5)

  • DNA directed RNA polymerase II 23 kda polypeptide
  • DNA-directed RNA polymerase II 23 kDa polypeptide
  • DNA-directed RNA polymerase II subunit E
  • DNA-directed RNA polymerases I, II, and III subunit RPABC1
  • hRPB25
  • hsRPB5
  • polymerase (RNA) II (DNA directed) polypeptide E (25kD)
  • polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
  • RPABC1
  • RPB5 homolog
  • RPB5
  • XAP4RNA polymerases I, II, and III subunit ABC1

Background

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for POLR2E Antibody (NBP2-00482)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for POLR2E Antibody (NBP2-00482)

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Bioinformatics

Gene Symbol POLR2E
Entrez