RPB9 Antibody

Images

 
Western Blot: RPB9 Antibody [NBP1-92344] - Analysis in human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: RPB9 Antibody [NBP1-92344] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RPB9 Antibody [NBP1-92344] - Staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
Western Blot: RPB9 Antibody [NBP1-92344] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: RPB9 Antibody [NBP1-92344] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RPB9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSA
Specificity
Specificity of human, mouse, rat RPB9 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2I
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RPB9 Protein (NBP1-92344PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPB9 Antibody

  • DNA-directed RNA polymerase II 14.5 kDa polypeptide
  • DNA-directed RNA polymerase II subunit I
  • DNA-directed RNA polymerase II subunit RPB9
  • hRPB14.5
  • polymerase (RNA) II (DNA directed) polypeptide I (14.5kD)
  • polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
  • RNA polymerase II 14.5 kDa subunit
  • RNA polymerase II subunit B9
  • RPB14.5
  • RPB9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol POLR2I