RHBDF1 Antibody - Azide and BSA Free

Western Blot: RHBDF1 Antibody [NBP2-94068] - Analysis of extracts of various cell lines, using RHBDF1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.

Immunocytochemistry/Immunofluorescence: RHBDF1 Antibody [NBP2-94068] - Analysis of L929 cells using RHBDF1 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RHBDF1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit RHBDF1 Antibody - Azide and BSA Free (NBP2-94068) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human RHBDF1 (NP_071895.3). HRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RHBDF1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for RHBDF1 Antibody - Azide and BSA Free

• C16orf8
• chromosome 16 open reading frame 8
• Dist1
• EGFR-RS
• epidermal growth factor receptor, related sequence
• FLJ2235
• FLJ22357
• gene-89
• gene-90
• hDist1
• p100hRho
• rhomboid 5 homolog 1 (Drosophila)
• Rhomboid 5 homolog 1
• rhomboid family 1 (Drosophila)
• rhomboid family 1
• rhomboid family member 1

Background

Not expected to have protease activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: RHBDF1