ErbB3/Her3 Antibody (66223)

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Recombinant Human NRG1‑1 beta 1/HRG1‑1 beta 1 (Catalog # 377-HB) stimulates proliferation in the MCF‑17 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ELISA(Cap)
Clone
66223
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ErbB3/Her3 Antibody (66223) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
Ser20-Thr643
Accession # P21860
Specificity
Detects human ErbB3/Her3 in ELISAs. Does not cross-react with recombinant EGF R.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ERBB3
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • Neutralization 0.0075-0.03 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human ErbB3/Her3 Biotinylated Antibody (Catalog number BAM348)
Standard: Recombinant Human ErbB3/Her3 Fc Chimera (Catalog number 348-RB)
Control
ErbB3/Her3 Lysate (NBP2-64690)
Publications
Read Publications using
MAB3481 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB3/Her3 Antibody (66223)

  • c-erbB3
  • EC 2.7.10
  • EC 2.7.10.1
  • ErbB3
  • ErbB-3
  • erbB3-S
  • HER3
  • HER3c-erbB-3
  • LCCS2
  • lethal congenital contracture syndrome 2
  • MDA-BF-1
  • MGC88033
  • p180-ErbB3
  • p45-sErbB3
  • p85-sErbB3
  • Proto-oncogene-like protein c-ErbB-3
  • receptor tyrosine-protein kinase erbB-3
  • Tyrosine kinase-type cell surface receptor HER3
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

  1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
  2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
  3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
  4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
  6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  9. Schlessinger, J. (2000) Cell 103:211.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB3/Her3 Antibody (MAB3481)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Capture, ELISA Development, Flow, IP, Neut, WB.


Filter By Application
ELISA Capture
(2)
ELISA Development
(3)
Flow
(1)
IP
(1)
Neut
(1)
WB
(1)
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Filter By Species
Human
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB3481 Applications Species
AA Memon, SC Gilliver, M Borre, J Sundquist, K Sundquist, E Nexo, BS Sorensen Soluble HER3 predicts survival in bladder cancer patients Oncol Lett, 2018;15(2):1783-1788. 2018 [PMID: 29434875] (ELISA Capture,, Human) ELISA Capture Human
C Le Clorenn, H Bazin, O Dubreuil, C Larbouret, C Ogier, Y Lazrek, V Garambois, MA Poul, P Mondon, JM Barret, G Mathis, JF Prost, A Pèlegrin, T Chardès Neuregulin 1 allosterically enhances the anti-tumor effects of the non-competing anti-HER3 antibody 9F7-F11 by increasing its binding to HER3 Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28507002] (ELISA Development,, Human) ELISA Development Human
Helmout Modjtahedi Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs Int. J. Oncol., 2016;49(5):1825-1838. 2016 [PMID: 27599579] (WB,, Human) WB Human
V Fock, K Plessl, P Draxler, GR Otti, C Fiala, M Knöfler, J Pollheimer Neuregulin-1-mediated ErbB2-ErbB3 signalling protects human trophoblasts against apoptosis to preserve differentiation J. Cell. Sci, 2015;128(23):4306-16. 2015 [PMID: 26490994] (Neut,, Human) Neut Human
Liu Z, Leng E, Gunasekaran K, Pentony M, Shen M, Howard M, Stoops J, Manchulenko K, Razinkov V, Liu H, Fanslow W, Hu Z, Sun N, Hasegawa H, Clark R, Foltz I, Yan W A novel antibody engineering strategy for making monovalent bispecific heterodimeric IgG antibodies by electrostatic steering mechanism. J Biol Chem, 2015;290(12):7535-62. 2015 [PMID: 25583986] (ELISA Capture,, Human) ELISA Capture Human
Khelwatty SA, Essapen S, Seddon AM, Modjtahedi H Growth response of human colorectal tumour cell lines to treatment with afatinib (BIBW2992), an irreversible erbB family blocker, and its association with expression of HER family members. Int. J. Oncol., 2011;39(2):483-91. 2011 [PMID: 21617858] (Flow,, Human) Flow Human
Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells. Assay Drug Dev Technol, 2010;8(1):27-36. 2010 [PMID: 20035613] (IP,, Human) IP Human
Lin SH, Lee YC, Choueiri MB, Wen S, Mathew P, Ye X, Do KA, Navone NM, Kim J, Tu SM, Yu-Lee LY, Logothetis CJ Soluble ErbB3 levels in bone marrow and plasma of men with prostate cancer. Clin. Cancer Res., 2008;14(12):3729-36. 2008 [PMID: 18559590] (ELISA Development,, Human) ELISA Development Human
Lin SH, Cheng CJ, Lee YC, Ye X, Tsai WW, Kim J, Pasqualini R, Arap W, Navone NM, Tu SM, Hu M, Yu-Lee LY, Logothetis CJ A 45-kDa ErbB3 secreted by prostate cancer cells promotes bone formation. Oncogene, 2008;27(39):5195-203. 2008 [PMID: 18490922] (ELISA Development,, Human) ELISA Development Human

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Bioinformatics

Gene Symbol ERBB3
Entrez
Uniprot