ErbB3/Her3 Antibody (66223)


Recombinant Human NRG1‑1 beta 1/HRG1‑1 beta 1 (Catalog # 377-HB) stimulates proliferation in the MCF‑17 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ELISA(Cap)

Order Details

ErbB3/Her3 Antibody (66223) Summary

Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
Accession # P21860
Detects human ErbB3/Her3 in ELISAs. Does not cross-react with recombinant EGF R.
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • Neutralization 0.0075-0.03 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human ErbB3/Her3 Biotinylated Antibody (Catalog number BAM348)
Standard: Recombinant Human ErbB3/Her3 Fc Chimera (Catalog number 348-RB)
Read Publications using
MAB3481 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB3/Her3 Antibody (66223)

  • c-erbB3
  • EC 2.7.10
  • EC
  • ErbB3
  • ErbB-3
  • erbB3-S
  • HER3
  • HER3c-erbB-3
  • LCCS2
  • lethal congenital contracture syndrome 2
  • MDA-BF-1
  • MGC88033
  • p180-ErbB3
  • p45-sErbB3
  • p85-sErbB3
  • Proto-oncogene-like protein c-ErbB-3
  • receptor tyrosine-protein kinase erbB-3
  • Tyrosine kinase-type cell surface receptor HER3
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)


ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

  1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
  2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
  3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
  4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
  6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  9. Schlessinger, J. (2000) Cell 103:211.
  10. Daly, R.J. (1999) Growth Factors 16:255.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB3/Her3 Antibody (MAB3481)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: Dynamic Light Scattering, ELISA Development, ELISA Development (Capture), Flow Cytometry, Immunoprecipitation, Neutralization, Western Blot.

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Dynamic Light Scattering
ELISA Development
ELISA Development (Capture)
Flow Cytometry
Western Blot
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB3481 Applications Species
T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer Oncol Rep, 2020;0(0):. 2020 [PMID: 33125153] (Flow Cytometry, Human) Flow Cytometry Human
J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv, 2020;6(9):eaax3223. 2020 [PMID: 32133394] (Western Blot, Human) Western Blot Human
J Wang, W Anderson, J Li, LL Lin, Y Wang, M Trau A high-resolution study of in situ surface-enhanced Raman scattering nanotag behavior in biological systems J Colloid Interface Sci, 2019;537(0):536-546. 2019 [PMID: 30469121] (Dynamic Light Scattering, Human) Dynamic Light Scattering Human
AA Memon, SC Gilliver, M Borre, J Sundquist, K Sundquist, E Nexo, BS Sorensen Soluble HER3 predicts survival in bladder cancer patients Oncol Lett, 2018;15(2):1783-1788. 2018 [PMID: 29434875] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
C Le Clorenn, H Bazin, O Dubreuil, C Larbouret, C Ogier, Y Lazrek, V Garambois, MA Poul, P Mondon, JM Barret, G Mathis, JF Prost, A Pèlegrin, T Chardès Neuregulin 1 allosterically enhances the anti-tumor effects of the non-competing anti-HER3 antibody 9F7-F11 by increasing its binding to HER3 Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28507002] (ELISA Development, Human) ELISA Development Human
Helmout Modjtahedi Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs Int. J. Oncol., 2016;49(5):1825-1838. 2016 [PMID: 27599579] (Western Blot, Human) Western Blot Human
V Fock, K Plessl, P Draxler, GR Otti, C Fiala, M Knöfler, J Pollheimer Neuregulin-1-mediated ErbB2-ErbB3 signalling protects human trophoblasts against apoptosis to preserve differentiation J. Cell. Sci, 2015;128(23):4306-16. 2015 [PMID: 26490994] (Neutralization, Human) Neutralization Human
Liu Z, Leng E, Gunasekaran K, Pentony M, Shen M, Howard M, Stoops J, Manchulenko K, Razinkov V, Liu H, Fanslow W, Hu Z, Sun N, Hasegawa H, Clark R, Foltz I, Yan W A novel antibody engineering strategy for making monovalent bispecific heterodimeric IgG antibodies by electrostatic steering mechanism. J Biol Chem, 2015;290(12):7535-62. 2015 [PMID: 25583986] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Khelwatty SA, Essapen S, Seddon AM, Modjtahedi H Growth response of human colorectal tumour cell lines to treatment with afatinib (BIBW2992), an irreversible erbB family blocker, and its association with expression of HER family members. Int. J. Oncol., 2011;39(2):483-91. 2011 [PMID: 21617858] (Flow Cytometry, Human) Flow Cytometry Human
Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells. Assay Drug Dev Technol, 2010;8(1):27-36. 2010 [PMID: 20035613] (Immunoprecipitation, Human) Immunoprecipitation Human
Show All 12 Publications.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB3481U
Alexa Fluor 405 FAB3481V
Alexa Fluor 488 FAB3481G
Alexa Fluor 488 FAB3481G
Alexa Fluor 532 FAB3481X
Alexa Fluor 594 FAB3481T
Alexa Fluor 647 FAB3481R
Alexa Fluor 700 FAB3481N
Alexa Fluor 750 FAB3481S
Allophycocyanin FAB3481A
Allophycocyanin FAB3481A
Biotin FAB3481B
DyLight 350 FAB3481D
DyLight 405 FAB3481E
DyLight 488 FAB3481K
DyLight 550 FAB3481L
DyLight 594 FAB3481M
DyLight 650 FAB3481W
DyLight 680 FAB3481Y
DyLight 755 FAB3481Z
Janelia Fluor 549 FAB3481I
Janelia Fluor 646 FAB3481J
Phycoerythrin FAB3481P
Phycoerythrin FAB3481P

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Gene Symbol ERBB3