Novus Biologicals products are now on

ErbB3/Her3 Antibody (66223)


Recombinant Human NRG1‑1 beta 1/HRG1‑1 beta 1 (Catalog # 377-HB) stimulates proliferation in the MCF‑17 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ELISA(Cap)

Order Details

ErbB3/Her3 Antibody (66223) Summary

Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
Accession # P21860
Detects human ErbB3/Her3 in ELISAs. Does not cross-react with recombinant EGF R.
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.0075-0.03 ug/mL
Application Notes
ELISA Detection: Human ErbB3/Her3 Biotinylated Antibody (Catalog number BAM348)
Standard: Recombinant Human ErbB3/Her3 Fc Chimera (Catalog number 348-RB)
Read Publications using
MAB3481 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB3/Her3 Antibody (66223)

  • c-erbB3
  • EC 2.7.10
  • EC
  • ErbB3
  • ErbB-3
  • erbB3-S
  • HER3
  • HER3c-erbB-3
  • LCCS2
  • lethal congenital contracture syndrome 2
  • MDA-BF-1
  • MGC88033
  • p180-ErbB3
  • p45-sErbB3
  • p85-sErbB3
  • Proto-oncogene-like protein c-ErbB-3
  • receptor tyrosine-protein kinase erbB-3
  • Tyrosine kinase-type cell surface receptor HER3
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)


ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

  1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
  2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
  3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
  4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
  6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  9. Schlessinger, J. (2000) Cell 103:211.
  10. Daly, R.J. (1999) Growth Factors 16:255.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu
Applications: ICC, IHC, WB
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IP, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
Species: Mu
Applications: WB
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Species: Hu
Applications: ICC, IHC, Neut, WB
Species: Hu
Applications: Flow, B/N, CyTOF-ready, ELISA(Cap)

Publications for ErbB3/Her3 Antibody (MAB3481)(21)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 8 applications: Dynamic Light Scattering, ELISA Development, ELISA Development (Capture), Flow Cytometry, Immunoprecipitation, Luminex Development, Neutralization, Western Blot.

Filter By Application
Dynamic Light Scattering
ELISA Development
ELISA Development (Capture)
Flow Cytometry
Luminex Development
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using MAB3481 Applications Species
Nana Lyu, Bernadette Pedersen, Elena Shklovskaya, Helen Rizos, Mark P. Molloy, Yuling Wang SERS characterization of colorectal cancer cell surface markers upon anti‐EGFR treatment Exploration (Beijing) 2022-06-01 [PMID: 37323700]
TW Moody, I Ramos-Alva, RT Jensen Adding of neurotensin to non-small cell lung cancer cells increases tyrosine phosphorylation of HER3 Peptides, 2022-08-03;156(0):170858. 2022-08-03 [PMID: 35932909] (Neutralization, Human) Neutralization Human
Ralph El Hallal, Nana Lyu, Yuling Wang Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells Molecules 2021-01-22 [PMID: 33499047]
T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer Oncol Rep, 2020-10-22;0(0):. 2020-10-22 [PMID: 33125153] (Flow Cytometry, Human) Flow Cytometry Human
Terry W. Moody, Lingaku Lee, Robert T. Jensen The G Protein–Coupled Receptor PAC1 Regulates Transactivation of the Receptor Tyrosine Kinase HER3 Journal of Molecular Neuroscience 2021-08-01 [PMID: 32964398]
J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv, 2020-02-26;6(9):eaax3223. 2020-02-26 [PMID: 32133394] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human
Lingaku Lee, Irene Ramos-Alvarez, Terry W. Moody, Samuel A. Mantey, Robert T. Jensen Neuropeptide bombesin receptor activation stimulates growth of lung cancer cells through HER3 with a MAPK-dependent mechanism. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2020-04-01 [PMID: 31862538]
J Wang, W Anderson, J Li, LL Lin, Y Wang, M Trau A high-resolution study of in situ surface-enhanced Raman scattering nanotag behavior in biological systems J Colloid Interface Sci, 2018-11-12;537(0):536-546. 2018-11-12 [PMID: 30469121] (Dynamic Light Scattering, Human) Dynamic Light Scattering Human
AA Memon, SC Gilliver, M Borre, J Sundquist, K Sundquist, E Nexo, BS Sorensen Soluble HER3 predicts survival in bladder cancer patients Oncol Lett, 2017-11-22;15(2):1783-1788. 2017-11-22 [PMID: 29434875] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Martin Pool, Arjan Kol, Steven de Jong, Elisabeth G. E. de Vries, Marjolijn N. Lub-de Hooge, Anton G.T. Terwisscha van Scheltinga 89Zr-mAb3481 PET for HER3 tumor status assessment during lapatinib treatment mAbs 2017-11-17 [PMID: 28873009]
Show All 21 Publications.

Reviews for ErbB3/Her3 Antibody (MAB3481) (0)

There are no reviews for ErbB3/Her3 Antibody (MAB3481). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB3/Her3 Antibody (MAB3481) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our ErbB3/Her3 Antibody (66223) and receive a gift card or discount.


Gene Symbol ERBB3