Prox1 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Prox1 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Prox1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Prox1 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Prox1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Prox1
  Pro2-Gln259
  Accession # Q92786
• Specificity: Detects human Prox1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PROX1

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prox1 Antibody [Unconjugated]

• Homeobox prospero-like protein PROX1
• prospero homeobox 1
• prospero homeobox protein 1
• prospero-related homeobox 1
• Prox1
• PROX-1

Background

Prox1, the homologue of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence homology with mouse and rat Prox1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prox1 Antibody (AF2727)(42)

Publications using AF2727

• S Gramolelli, J Cheng, I Martinez-C, M Vähä-Koske, E Elbasani, E Kaivanto, V Rantanen, K Tuohinto, S Hautaniemi, M Bower, C Haglund, K Alitalo, T Mäkinen, TV Petrova, K Lehti, PM Ojala PROX1 is a transcriptional regulator of MMP14 Sci Rep, 2018;8(1):9531. 2018 [PMID: 29934628] (ChIP,, Mouse)
• M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program Nat Commun, 2018;9(1):1511. 2018 [PMID: 29666442] (IHC,, Mouse)
• M Niquille, G Limoni, F Markopoulo, C Cadilhac, J Prados, A Holtmaat, A Dayer Neurogliaform cortical interneurons derive from cells in the preoptic area Elife, 2018;7(0):. 2018 [PMID: 29557780] (IHC-Fr,, Mouse)
• M Rudzinska, JK Ledwon, D Gawel, J Sikorska, B Czarnocka The role of prospero homeobox 1 (PROX1) expression in follicular thyroid carcinoma cells Oncotarget, 2017;8(69):114136-114155. 2017 [PMID: 29371975] (WB,, Human)
• S Rhee, JI Chung, DA King, G D'amato, DT Paik, A Duan, A Chang, D Nagelberg, B Sharma, Y Jeong, M Diehn, JC Wu, AJ Morrison, K Red-Horse Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease Nat Commun, 2018;9(1):368. 2018 [PMID: 29371594] (IHC,, Mouse)
• J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28920924] (WB,, Human)
• YC Lin, N Ohbayashi, T Hongu, N Katagiri, Y Funakoshi, H Lee, Y Kanaho Arf6 in lymphatic endothelial cells regulates lymphangiogenesis by controlling directional cell migration Sci Rep, 2017;7(1):11431. 2017 [PMID: 28900118] (IHC,, Mouse)
• A Laitinen, C Böckelman, J Hagström, A Kokkola, P Kallio, C Haglund High PROX1 expression in gastric cancer predicts better survival PLoS ONE, 2017;12(8):e0183868. 2017 [PMID: 28854215] (IHC Paraffin-embedded,, Human)
• R Hägerling, D Drees, A Scherzinge, C Dierkes, S Martin-Alm, S Butz, K Gordon, M Schäfers, K Hinrichs, P Ostergaard, D Vestweber, T Goerge, S Mansour, X Jiang, PS Mortimer, F Kiefer VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations JCI Insight, 2017;2(16):. 2017 [PMID: 28814672] (IHC-Fr,, Human)
• Y Mimura-Yam, H Shinohara, T Kashiwagi, T Sato, S Shioda, T Seki Dynamics and function of CXCR4 in formation of the granule cell layer during hippocampal development Sci Rep, 2017;7(1):5647. 2017 [PMID: 28717168] (IHC,, Mouse)
• E Jung, D Gardner, D Choi, E Park, Y Jin Seong, S Yang, J Castorena-, A Louveau, Z Zhou, GK Lee, DP Perrault, S Lee, M Johnson, G Daghlian, M Lee, Y Jin Hong, Y Kato, J Kipnis, MJ Davis, AK Wong, YK Hong Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm's Canal Reporter Rat Sci Rep, 2017;7(1):5577. 2017 [PMID: 28717161] (IHC,, Rat)
• SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1 Sci Rep, 2017;7(1):4916. 2017 [PMID: 28687807] (ICC,, Human)
• KS Yan, O Gevaert, GXY Zheng, B Anchang, CS Probert, KA Larkin, PS Davies, ZF Cheng, JS Kaddis, A Han, K Roelf, RI Calderon, E Cynn, X Hu, K Mandleywal, J Wilhelmy, SM Grimes, DC Corney, SC Boutet, JM Terry, P Belgrader, SB Ziraldo, TS Mikkelsen, F Wang, RJ von Furste, NR Smith, P Chandrakes, R May, MAS Chrissy, R Jain, CA Cartwright, JC Niland, YK Hong, J Carrington, DT Breault, J Epstein, CW Houchen, JP Lynch, MG Martin, SK Plevritis, C Curtis, HP Ji, L Li, SJ Henning, MH Wong, CJ Kuo Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity Cell Stem Cell, 2017;21(1):78-90.e6. 2017 [PMID: 28686870] (IHC Frozen,, Mouse)
• LD Volk-Drape, KL Hall, AC Wilber, S Ran Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4 PLoS ONE, 2017;12(6):e0179257. 2017 [PMID: 28598999] (ICC,, Human)
• J Matsushita, S Inagaki, T Nishie, T Sakasai, J Tanaka, C Watanabe, KI Mizutani, Y Miwa, K Matsumoto, K Takara, H Naito, H Kidoya, N Takakura, T Nagai, S Takahashi, M Ema Fluorescence and Bioluminescence Imaging of Angiogenesis in Flk1-Nano-lantern Transgenic Mice Sci Rep, 2017;7(0):46597. 2017 [PMID: 28425492] (IHC OCT-embedded,, Mouse)
• JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray Human umbilical cord plasma proteins revitalize hippocampal function in aged mice Nature, 2017;544(7651):488-492. 2017 [PMID: 28424512] (IHC Frozen,, Mouse)
• JJ Teoh, T Iwano, M Kunii, N Atik, E Avriyanti, SI Yoshimura, K Moriwaki, A Harada BIG1 is required for the survival of deep layer neurons, neuronal polarity, and the formation of axonal tracts between the thalamus and neocortex in developing brain PLoS ONE, 2017;12(4):e0175888. 2017 [PMID: 28414797] (IHC OCT-embedded,, Mouse)
• S Frazer, J Prados, M Niquille, C Cadilhac, F Markopoulo, L Gomez, U Tomasello, L Telley, A Holtmaat, D Jabaudon, A Dayer Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing Nat Commun, 2017;8(0):14219. 2017 [PMID: 28134272] (IHC Frozen,, Mouse)
• M Malinverno, M Corada, L Ferrarini, L Formicola, G Marazzi, D Sassoon, E Dejana Peg3/PW1 is a marker of a subset of vessel associated endothelial progenitors Stem Cells, 2017;0(0):. 2017 [PMID: 28090691] (IHC Fresh, Mouse)
• Gene-expression profiling of different arms of lymphatic vasculature identifies candidates for manipulation of cell traffic Proc Natl Acad Sci USA, 2016;113(38):10643-10648. 2016 [PMID: 27601677] (IHC Not Specified,, Mouse)
• Lymphatic vessels regulate immune microenvironments in human and murine melanoma J Clin Invest, 2016;0(0):. 2016 [PMID: 27525437] (IHC Paraffin-embedded,, Human)
• Boksik Cha Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves Genes Dev, 2016;30(12):1454-69. 2016 [PMID: 27313318] (WB,, Human)
• Foxc1 and Foxc2 deletion causes abnormal lymphangiogenesis and correlates with ERK hyperactivation J Clin Invest, 2016;0(0):. 2016 [PMID: 27214551] (Flow,, Mouse)
• CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity J Clin Invest, 2016;0(0):. 2016 [PMID: 27140400] (IHC Paraffin-embedded,, Mouse)
• Geng R, Noda T, Mulvaney J, Lin V, Edge A, Dabdoub A Comprehensive Expression of Wnt Signaling Pathway Genes during Development and Maturation of the Mouse Cochlea. PLoS ONE, 2015;11(2):e0148339. 2015 [PMID: 26859490] (IHC Not Specified,, Mouse)
• Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development. Dev Biol, 2016;409(1):218-33. 2016 [PMID: 26542011] (IHC-OCT embedded,, Mouse)
• Aspelund A, Antila S, Proulx S, Karlsen T, Karaman S, Detmar M, Wiig H, Alitalo K A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules. J Exp Med, 2015;212(7):991-9. 2015 [PMID: 26077718] (IHC Not Specified,, Mouse)
• Bianchi R, Teijeira A, Proulx S, Christiansen A, Seidel C, Rulicke T, Makinen T, Hagerling R, Halin C, Detmar M A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research. PLoS ONE, 2015;10(4):e0122976. 2015 [PMID: 25849579] (IHC Fresh,, Mouse)
• Van't Hull E, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye J, Doucey M Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer. Oncoimmunology, 2014;3(0):e29080. 2014 [PMID: 25101222] (ICC,, Human)
• Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel. J Clin Invest, 2014;124(9):3975-86. 2014 [PMID: 25061878] (IHC Paraffin-embedded,, Human)
• Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity. J Clin Invest, 2014;124(9):3960-74. 2014 [PMID: 25061877]
• Zheng W, Nurmi H, Appak S, Sabine A, Bovay E, Korhonen E, Orsenigo F, Lohela M, D'Amico G, Holopainen T, Leow C, Dejana E, Petrova T, Augustin H, Alitalo K Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions. Genes Dev, 2014;28(14):1592-603. 2014 [PMID: 25030698] (IHC Frozen,, Mouse)
• Fatima A, Culver A, Culver F, Liu T, Dietz W, Thomson B, Hadjantonakis A, Quaggin S, Kume T Murine Notch1 is required for lymphatic vascular morphogenesis during development. Dev Dyn, 2014;243(7):957-64. 2014 [PMID: 24659232] (IHC - Not specified,, Mouse)
• Brzezinski J, Uoon Park K, Reh T Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence. Dev Biol, 2013;384(2):194-204. 2013 [PMID: 24125957] (IHC Frozen,, Mouse)
• Sugiyama T, Osumi N, Katsuyama Y The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages. Dev Dyn, 2013;242(12):1442-53. 2013 [PMID: 24038449] (immunohistochemistry,, Mouse)
• Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. EMBO J, 2013;32(5):629-44. 2013 [PMID: 23299940] (IHC OCT-embedded,, Mouse)
• Aberrant mural cell recruitment to lymphatic vessels and impaired lymphatic drainage in a murine model of pulmonary fibrosis. Blood, 2012;119(24):5931-42. 2012 [PMID: 22547584] (ICC,, Mouse)
• Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival. Blood, 2012;119(24):5850-8. 2012 [PMID: 22374700] (IHC,, Mouse)
• Skog M, Bono P, Lundin M, Lundin J, Louhimo J, Linder N, Petrova TV, Andersson LC, Joensuu H, Alitalo K, Haglund CH Expression and prognostic value of transcription factor PROX1 in colorectal cancer. Br. J. Cancer, 2011;105(9):1346-51. 2011 [PMID: 21970873] (IHC Paraffin-embedded,, Human)
• Elyada E, Pribluda A, Goldstein RE, Morgenstern Y, Brachya G, Cojocaru G, Snir-Alkalay I, Burstain I, Haffner-Krausz R, Jung S, Wiener Z, Alitalo K, Oren M, Pikarsky E, Ben-Neriah Y CKIalpha ablation highlights a critical role for p53 in invasiveness control. Nature, 2011;470(7334):409-13. 2011 [PMID: 21331045] (WB,, Mouse)
• Philipp M, Fralish GB, Meloni AR, Chen W, MacInnes AW, Barak LS, Caron MG Smoothened signaling in vertebrates is facilitated by a G protein-coupled receptor kinase. Mol. Biol. Cell, 2008;19(12):5478-89. 2008 [PMID: 18815277] (IHC,, Zebrafish)
• Heckman CA, Holopainen T, Wirzenius M, Keskitalo S, Jeltsch M, Yla-Herttuala S, Wedge SR, Jurgensmeier JM, Alitalo K The tyrosine kinase inhibitor cediranib blocks ligand-induced vascular endothelial growth factor receptor-3 activity and lymphangiogenesis. Cancer Res., 2008;68(12):4754-62. 2008 [PMID: 18559522] (ICC,, Human)

Reviews for Prox1 Antibody (AF2727) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Other.

reviewed by: Wei-Sheng Chen WB Human 07/13/2015

Summary

• Application: Western Blot
• Sample Tested: primary human lymphatic endothelial cells
• Species: Human

Blocking

• Blocking Details: 0.5x Odyssey's blocking buffer, RT, 30 min

Primary Anitbody

• Dilution Ratio: 1,000 dilution in 0.5x Odyssey's blocking buffer, 4C, overnight.

Secondary Antibody

• Secondary Description: Donkey anti-goat IgG, 10,000 dilution in 0.5x Odyssey's blocking buffer, RT, 45 min.

Details

• Detection Notes: Odyssey® CLx Infrared Imaging System

reviewed by: Anonymous IF Mouse 01/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23299940
• Species: Mouse

Blocking

• Blocking Details: See PMID 23299940

Details

• Detection Notes: See PMID 23299940

Comments

• Comments: Published: See PMID 23299940

reviewed by: Anonymous IHC-P Other 01/07/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22115316
• Species: Other

Bioinformatics

• Gene Symbol: PROX1
• Entrez:
  • 5629(Human)
• Uniprot:
  • Q92786()