Prox1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by ...read more
Prox1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) at 15 µg/mL for 3 hours at room ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Prox1 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat ...read more
RNA-seq identifies the targets of GATA2 in primary human LECs. (A) Principal component analysis (PCA) was performed on RNA-seq data from control shRNA- and shGATA2-infected primary HLECs. A high level of similarity was ...read more
Prox1 regulates myoblast differentiation.(a) Analysis of Prox1 mRNA and protein during C2C12 myoblast differentiation (indicated by the increase in MyoD1 mRNA). (b) Prox1 protein and mRNA in human myoblasts before and ...read more
In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. ...read more
In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. ...read more
Prox1 regulates the slow muscle fibre gene program in skeletal muscle.(a) MyHC I (Myh7) expression in muscle fibres (arrows) in AAV8-Prox1 and AAV8-Ctrl transduced TA muscle. (b) Expression of myosin heavy chain and ...read more
In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Prox1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Prox1
Pro2-Gln259
Accession # Q92786
Specificity
Detects human Prox1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PROX1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 4 Reviews rated 4.8
using
AF2727 in the following applications:

Publications
Read Publications using
AF2727 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prox1 Antibody [Unconjugated]

  • Homeobox prospero-like protein PROX1
  • prospero homeobox 1
  • prospero homeobox protein 1
  • prospero-related homeobox 1
  • Prox1
  • PROX-1

Background

Prox1, the homologue of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence homology with mouse and rat Prox1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF743
Species: Mu
Applications: CyTOF-ready, Flow, WB
AF2125
Species: Mu
Applications: IHC, WB
DVEC00
Species: Hu
Applications: ELISA
NB300-750
Species: Fi, Hu, Pm, Mu, Pm, Rt, Sh
Applications: ICC/IF, IHC, IP, WB
NBP1-89917
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
DVE00
Species: Hu
Applications: ELISA
802-HC/CF
Species: Hu
Applications: BA, BA
NBP2-27196
Species: Bv, Ca, Ch, ChHa, Eq, Gp, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, RM, Ze
Applications: IHC, IHC-P, KD, WB
NBP2-24551
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
AF2018
Species: Hu, Mu, Rt
Applications: ChIP, ICC, IHC, Simple Western, WB
H00006496-M04
Species: Hu
Applications: ELISA, WB
NB110-58867
Species: Bv, Hu, Mu, Po, Rt
Applications: ICC/IF, WB
NB110-41083
Species: Hu
Applications: ChIP, ELISA, ICC/IF, WB
AF3628
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP3-10932
Species: Hu
Applications: IHC, WB
DVED00
Species: Hu
Applications: ELISA
AF2567
Species: Mu
Applications: IHC, WB
AF2727
Species: Hu
Applications: WB, Simple Western, ICC/IF

Publications for Prox1 Antibody (AF2727)(84)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Transgenic Mouse, Xenograft, Zebrafish.

We have publications tested in 8 applications: ChIP, Flow Cytometry, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ChIP
(1)
Flow Cytometry
(2)
ICC
(9)
ICC/IF
(1)
IHC
(53)
IHC-Fr
(9)
IHC-P
(12)
Western Blot
(10)
All Applications
Filter By Species
Human
(25)
Mouse
(58)
Rat
(2)
Chicken
(1)
Transgenic Mouse
(3)
Xenograft
(2)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 84. Show All 84 Publications.
Publications using AF2727 Applications Species
Jannaway, M;Iyer, D;Mastrogiacomo, DM;Li, K;Sung, DC;Yang, Y;Kahn, ML;Scallan, JP; VEGFR3 is required for button junction formation in lymphatic vessels Cell reports 2023-07-15 [PMID: 37454290] (IHC, Mouse) IHC Mouse
Ohata, H;Shiokawa, D;Sakai, H;Kanda, Y;Okimoto, Y;Kaneko, S;Hamamoto, R;Nakagama, H;Okamoto, K; PROX1 induction by autolysosomal activity stabilizes persister-like state of colon cancer via feedback repression of the NOX1-mTORC1 pathway Cell reports 2023-05-17 [PMID: 37224811] (IHC, Xenograft, Human) IHC Xenograft, Human
PV Blinkiewic, MR Long, ZA Stoner, EM Ketchum, SN Sheltz-Kem, JS Duncan Gata3 is Required in Late Proneurosensory Development for Proper Sensory Cell Formation and Organization Research square, 2023-04-14;0(0):. 2023-04-14 [PMID: 37090645] (IHC, Mouse) IHC Mouse
J Kazenwadel, P Venugopal, A Oszmiana, J Toubia, L Arriola-Ma, V Panara, SG Piltz, C Brown, W Ma, AW Schreiber, K Koltowska, S Taoudi, PQ Thomas, HS Scott, NL Harvey A Prox1 enhancer represses haematopoiesis in the lymphatic vasculature Nature, 2023-01-25;614(7947):343-348. 2023-01-25 [PMID: 36697821] (IHC, Mouse) IHC Mouse
SH Lee, N Kim, M Kim, SH Woo, I Han, J Park, K Kim, KS Park, K Kim, D Shim, SE Park, JY Zhang, DM Go, DY Kim, WK Yoon, SP Lee, J Chung, KW Kim, JH Park, SH Lee, S Lee, SJ Ann, SH Lee, HS Ahn, SC Jeong, TK Kim, GT Oh, WY Park, HO Lee, JH Choi Single-cell transcriptomics reveal cellular diversity of aortic valve and the immunomodulation by PPARgamma during hyperlipidemia Nature Communications, 2022-09-17;13(1):5461. 2022-09-17 [PMID: 36115863] (IHC, Mouse) IHC Mouse
E Place, E Manning, DW Kim, A Kinjo, G Nakamura, K Ohyama SHH and Notch regulate SOX9+ progenitors to govern arcuate POMC neurogenesis Frontiers in Neuroscience, 2022-08-11;16(0):855288. 2022-08-11 [PMID: 36033614] (ICC/IF, Chicken) ICC/IF Chicken
FK Wong, M Selten, C Rosés-Nove, V Sreenivasa, N Pallas-Baz, E Serafeimid, A Hanusz-God, F Oozeer, R Edwards, O Marín Serotonergic regulation of bipolar cell survival in the developing cerebral cortex Cell Reports, 2022-07-05;40(1):111037. 2022-07-05 [PMID: 35793629] (IHC, Mouse) IHC Mouse
AE Hall, SÖ Pohl, P Cammareri, S Aitken, NT Younger, M Raponi, CV Billard, AB Carrancio, A Bastem, P Freile, F Haward, IR Adams, JF Caceres, P Preyzner, A von Kriegs, MG Dunlop, FV Din, KB Myant RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer Nature Communications, 2022-05-19;13(1):2791. 2022-05-19 [PMID: 35589755] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
A Choudhury, ST Magill, CD Eaton, BC Prager, WC Chen, MA Cady, K Seo, CG Lucas, TJ Casey-Clyd, HN Vasudevan, SJ Liu, JE Villanueva, TC Lam, JK Pu, LF Li, GK Leung, DL Swaney, MY Zhang, JW Chan, Z Qiu, MV Martin, MS Susko, SE Braunstein, NAO Bush, JD Schulte, N Butowski, PK Sneed, MS Berger, NJ Krogan, A Perry, JJ Phillips, DA Solomon, JF Costello, MW McDermott, JN Rich, DR Raleigh Meningioma DNA methylation groups identify biological drivers and therapeutic vulnerabilities Nature Genetics, 2022-05-09;54(5):649-659. 2022-05-09 [PMID: 35534562] (IHC, Human, Xenograft) IHC Human, Xenograft
I Genescu, M Aníbal-Mar, V Kouskoff, N Chenouard, C Mailhes-Ha, H Cartonnet, L Lokmane, FM Rijli, G López-Bend, F Gambino, S Garel Dynamic interplay between thalamic activity and Cajal-Retzius cells regulates the wiring of cortical layer 1 Cell Reports, 2022-04-12;39(2):110667. 2022-04-12 [PMID: 35417707] (IHC, Mouse) IHC Mouse
Show All 84 Publications.

Reviews for Prox1 Antibody (AF2727) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Human, Mouse, Other, Rabbit.

Reviews using AF2727:
Filter by Applications
IHC-Fr
(1)
WB
(1)
IF
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Other
(1)
Rabbit
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Prox1 AF2727
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Rabbit 07/07/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedbrain hippocampus section,Rabbit Brain dentate gyrus section
SpeciesRabbit
LotVIY0218061
Western Blot Prox1 AF2727
Enlarge
5
reviewed by:
Wei-Sheng Chen
WB Human 07/13/2015
View

Summary

ApplicationWestern Blot
Sample Testedprimary human lymphatic endothelial cells
SpeciesHuman
  5
reviewed by:
Verified Customer
IF Mouse 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23299940
SpeciesMouse
  4
reviewed by:
Verified Customer
IHC-P Other 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22115316
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Prox1 Antibody (AF2727) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Prox1 Products

Diseases for Prox1 Antibody (AF2727)

Discover more about diseases related to Prox1 Antibody (AF2727).
 

Pathways for Prox1 Antibody (AF2727)

View related products by pathway.

PTMs for Prox1 Antibody (AF2727)

Learn more about PTMs related to Prox1 Antibody (AF2727).

Blogs on Prox1.


  Read full blog post.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Verified Customer
07/07/2020
Application: IHC-Fr
Species: Rabbit

Wei-Sheng Chen
07/13/2015
Application: WB
Species: Human

 
Verified Customer
01/07/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol PROX1
Uniprot