Prox1 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Prox1 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Prox1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Prox1 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

RNA-seq identifies the targets of GATA2 in primary human LECs. (A) Principal component analysis (PCA) was performed on RNA-seq data from control shRNA- and shGATA2-infected primary HLECs. A high level of similarity was observed within the groups as indicated by their proximity to each other. (B) Hierarchical clustering shows that approximately 1000 genes were consistently downregulated and 600 genes were upregulated in shGATA2-treated HLECs. (C) GO revealed a list of genes that are likely relevant to the phenotypes observed in mice lacking GATA2. (D) GATA2 was knocked out from a second HLEC line using CRISPR/Cas9. Western blot revealed the lack of GATA2 in the knockout cells (HLEC delta GATA2). In contrast, no obvious differences were observed in the expression of PROX1. Additionally, qRT-PCR revealed the downregulation of miR-126. (A) n=3 independent experiments per shRNA; (D) n=3 independent experiments (antibiotic selection, western blot and qRT-PCR). **P<0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31582413), licensed under a CC-BY license. Not internally tested by R&D Systems.

Prox1 regulates myoblast differentiation.(a) Analysis of Prox1 mRNA and protein during C2C12 myoblast differentiation (indicated by the increase in MyoD1 mRNA). (b) Prox1 protein and mRNA in human myoblasts before and after differentiation. Primary myoblast lines from two different individuals were analysed. (c–f) Analysis of myoblast proliferation and differentiation-related CyclinD1, Myf5 and MyoD mRNAs in shProx1 and shScr transduced primary human myoblasts before (c) and after differentiation for 7d (d), as well as in mouse C2C12 myoblasts before (e) and after (f) differentiation. (g) Myosin staining of C2C12 cells after 7d of differentiation. Note that Prox1 silencing completely blocked myotube development, as only occasional cells expressed myosin and these cells were negative for shProx1-GFP. Data is presented as mean±s.e.m., n=3+3 biological replicates in a–g (all experiments were repeated three times). One-way analysis of variance with repeated measures followed by Tukey's posthoc test and Student's two-tailed unpaired t-test, *P<0.05, **P<0.01, ***P<0.001. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13124), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Prox1 regulates the slow muscle fibre gene program in skeletal muscle.(a) MyHC I (Myh7) expression in muscle fibres (arrows) in AAV8-Prox1 and AAV8-Ctrl transduced TA muscle. (b) Expression of myosin heavy chain and calcium-signalling genes in AAV8-Prox1 and AAV8-Ctrl transduced TA muscle. Note that the expression of slow fibre -specific genes are upregulated and fast MyHC genes downregulated in Prox1 overexpressing muscles. (c) Heat map from GSEA analysis showing that Prox1 overexpression in TA muscle leads to expression of many soleus specific myofibrillar genes (red colour indicates positive and blue negative enrichment). (d) Effect of Prox1 deletion on soleus muscle gene expression (HSA-CreERT2;Prox1fl/fl mice). Note the increased expression of fast MyHCs Myh2 and Myh4 RNAs, whereas slow troponin isoform RNAs and Myh7 RNA are decreased. (e) Deletion of Prox1 in slow soleus muscle with AAV-Cre leads to marked downregulation of MyHC I protein. Data is presented as mean±s.e.m., n=5+5 in a,b, n=3+3 in c, n=4+4 in d,e. Student's two-tailed unpaired t-test, *P<0.05, **P<0.01, ***P<0.001. Both overexpression and deletion experiments were conducted two times on independent mouse cohorts. WT, wild-type. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13124), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

TLS formation in models of heart failure, conditional lymphatic-EC deletion of Rbpj or kidney ischemia reperfusion. D PAS staining of representative, paraffin-embedded kidney sections. Quantification of infiltrated area [in mm2] per transversal kidney cross-section (sum of all infiltrated areas per section), N = 8 biological replicates per group, Mann–Whitney test, two-tailed, p = 0.51. Graph: Scatter dot blot, Mean, SD (whiskers). ERbpj delta EC Whole kidney staining for B220 (TLS)&Prox1/Lyve1 for lymphatic collecting vessels, light sheet microscopy, ventral view, 3D reconstruction via IMARIS software; scale bar: left image 1000 µm; insets are magnifications of boxed detail, scale bar 150 µm. Exemplary image, kidneys from N = 3 mice stained. F Induction protocol for lymphatic endothelial-restricted deletion of Rbpj (Rbpj delta LEC). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35440634), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Prox1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Prox1
  Pro2-Gln259
  Accession # Q92786
• Specificity: Detects human Prox1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PROX1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prox1 Antibody [Unconjugated]

• Homeobox prospero-like protein PROX1
• prospero homeobox 1
• prospero homeobox protein 1
• prospero-related homeobox 1
• Prox1
• PROX-1

Background

Prox1, the homologue of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence homology with mouse and rat Prox1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prox1 Antibody (AF2727)(152)

Publications using AF2727

• Fernandes, LM;Griswold-Wheeler, D;Tresemer, JD;Vallejo, A;Vishlaghi, N;Levi, B;Shapiro, A;Scallan, JP;Dellinger, MT; A single-cell atlas of normal and KRASG12D-malformed lymphatic vessels JCI insight 2025-01-28 [PMID: 39874106] (Immunohistochemistry, Mouse)
• Spurgin, S;Nguimtsop, AM;Chaudhry, FN;Michki, SN;Salvador, J;Iruela-Arispe, ML;Zepp, JA;Mukhopadhyay, S;Cleaver, O; Spatiotemporal dynamics of primary and motile cilia throughout lung development bioRxiv : the preprint server for biology 2024-10-26 [PMID: 39484464] (Immunohistochemistry, Transgenic Mouse)
• Nouruzi, S;Namekawa, T;Tabrizian, N;Kobelev, M;Sivak, O;Scurll, JM;Cui, CJ;Ganguli, D;Zoubeidi, A; ASCL1 regulates and cooperates with FOXA2 to drive terminal neuroendocrine phenotype in prostate cancer JCI insight 2024-12-06 [PMID: 39470735] (Western Blot, Human)
• Crossey, E;Carty, S;Shao, F;Henao-Vasquez, J;Ysasi, AB;Zeng, M;Hinds, A;Lo, M;Tilston-Lunel, A;Varelas, X;Jones, MR;Fine, A; Influenza induces lung lymphangiogenesis independent of YAP/TAZ activity in lymphatic endothelial cells. Scientific reports 2024-09-12 [PMID: 39266641] (Immunohistochemistry, Mouse)
• Arroyo-Ataz, G;Yagüe, AC;Breda, JC;Mazzilli, SA;Jones, D; Transcriptional, developmental, and functional parallels of lymphatic and venous smooth muscle bioRxiv : the preprint server for biology 2024-07-22 [PMID: 39091770] (Immunohistochemistry, Transgenic Mouse)
• Manolis, D;Hasan, S;Maraveyas, A;O'Brien, DP;Kessler, BM;Kramer, H;Nikitenko, LL; Quantitative proteomics reveals CLR interactome in primary human cells The Journal of biological chemistry 2024-05-20 [PMID: 38777147] (Immunocytochemistry, Human)
• Crossey, E;Carty, S;Shao, F;Henao-Vasquez, J;Ysasi, AB;Zeng, M;Hinds, A;Lo, M;Tilston-Lunel, A;Varelas, X;Jones, MR;Fine, A; Influenza Induces Lung Lymphangiogenesis Independent of YAP/TAZ Activity in Lymphatic Endothelial Cells Research square 2024-02-27 [PMID: 38463972] (Immunohistochemistry, Mouse)
• Sun, Q;Li, J;Ma, J;Zheng, Y;Ju, R;Li, X;Ren, X;Huang, L;Chen, R;Tan, X;Luo, L; JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens Fiber Maturation in Murine Lens Development Investigative ophthalmology & visual science 2023-12-01 [PMID: 38095908] (IHC, Mouse)
• Agnes Lee Chen Ong, Toshiya Kokaji, Arisa Kishi, Yoshihiro Takihara, Takuma Shinozuka, Ren Shimamoto et al. Acquisition of neural fate by combination of BMP blockade and chromatin modification iScience 2023-10-20 [PMID: 37771660]
• Swati Jain, John J. LaFrancois, Kasey Gerencer, Justin J. Botterill, Meghan Kennedy, Chiara Criscuolo et al. Increasing adult neurogenesis protects mice from epilepsy bioRxiv 2023-07-14 [PMID: 37502909]
• Jannaway, M;Iyer, D;Mastrogiacomo, DM;Li, K;Sung, DC;Yang, Y;Kahn, ML;Scallan, JP; VEGFR3 is required for button junction formation in lymphatic vessels Cell reports 2023-07-15 [PMID: 37454290] (IHC, Mouse)
• Ohata, H;Shiokawa, D;Sakai, H;Kanda, Y;Okimoto, Y;Kaneko, S;Hamamoto, R;Nakagama, H;Okamoto, K; PROX1 induction by autolysosomal activity stabilizes persister-like state of colon cancer via feedback repression of the NOX1-mTORC1 pathway Cell reports 2023-05-17 [PMID: 37224811] (IHC, Xenograft, Human)
• PV Blinkiewic, MR Long, ZA Stoner, EM Ketchum, SN Sheltz-Kem, JS Duncan Gata3 is Required in Late Proneurosensory Development for Proper Sensory Cell Formation and Organization Research square, 2023-04-14;0(0):. 2023-04-14 [PMID: 37090645] (IHC, Mouse)
• D Greene, Genomics E, D Pirri, K Frudd, E Sackey, M Al-Owain, APJ Giese, K Ramzan, S Riaz, I Yamanaka, N Boeckx, C Thys, BD Gelb, P Brennan, V Hartill, J Harvengt, T Kosho, S Mansour, M Masuno, T Ohata, H Stewart, K Taibah, CLS Turner, F Imtiaz, S Riazuddin, T Morisaki, P Ostergaard, BL Loeys, H Morisaki, ZM Ahmed, GM Birdsey, K Freson, A Mumford, E Turro Genetic association analysis of 77,539 genomes reveals rare disease etiologies Nature Medicine, 2023-03-16;29(3):679-688. 3/16/2023 [PMID: 36928819]
• Ivy K N Chiang, Matthew S Graus, Nils Kirschnick, Tara Davidson, Winnie Luu, Richard Harwood et al. The blood vasculature instructs lymphatic patterning in a SOX7‐dependent manner The EMBO Journal 2023-03-01 [PMID: 36715213] (Immunohistochemistry, Mouse)
• J Kazenwadel, P Venugopal, A Oszmiana, J Toubia, L Arriola-Ma, V Panara, SG Piltz, C Brown, W Ma, AW Schreiber, K Koltowska, S Taoudi, PQ Thomas, HS Scott, NL Harvey A Prox1 enhancer represses haematopoiesis in the lymphatic vasculature Nature, 2023-01-25;614(7947):343-348. 2023-01-25 [PMID: 36697821] (IHC, Mouse)
• Guillermo Arroyo-Ataz, Dennis Jones Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells STAR Protocols 2022-12-01 [PMID: 36353362] (Immunohistochemistry, Mouse)
• Fernando Bonet, Sabrina Brito Añez, José Manuel Inácio, Matthias E. Futschik, José Antonio Belo CCBE1 Is Essential for Epicardial Function during Myocardium Development International Journal of Molecular Sciences 2022-10-20 [PMID: 36293499]
• Wakao S, Oguma Y, Kushida Y et al. Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame Research Square 3/8/2022 [PMID: 36203068]
• Kazuaki Maruyama, Sachiko Miyagawa-Tomita, Yuka Haneda, Mayuko Kida, Fumio Matsuzaki, Kyoko Imanaka-Yoshida et al. The cardiopharyngeal mesoderm contributes to lymphatic vessel development in mouse eLife 2022-10-05 [PMID: 36196867]
• SH Lee, N Kim, M Kim, SH Woo, I Han, J Park, K Kim, KS Park, K Kim, D Shim, SE Park, JY Zhang, DM Go, DY Kim, WK Yoon, SP Lee, J Chung, KW Kim, JH Park, SH Lee, S Lee, SJ Ann, SH Lee, HS Ahn, SC Jeong, TK Kim, GT Oh, WY Park, HO Lee, JH Choi Single-cell transcriptomics reveal cellular diversity of aortic valve and the immunomodulation by PPARgamma during hyperlipidemia Nature Communications, 2022-09-17;13(1):5461. 2022-09-17 [PMID: 36115863] (IHC, Mouse)
• E Place, E Manning, DW Kim, A Kinjo, G Nakamura, K Ohyama SHH and Notch regulate SOX9+ progenitors to govern arcuate POMC neurogenesis Frontiers in Neuroscience, 2022-08-11;16(0):855288. 2022-08-11 [PMID: 36033614] (ICC/IF, Chicken)
• Y Zhou, Y Su, S Li, BC Kennedy, DY Zhang, AM Bond, Y Sun, F Jacob, L Lu, P Hu, AN Viaene, I Helbig, SK Kessler, T Lucas, RD Salinas, X Gu, HI Chen, H Wu, JE Kleinman, TM Hyde, DW Nauen, DR Weinberger, GL Ming, H Song Molecular landscapes of human hippocampal immature neurons across lifespan Nature, 2022-07-06;0(0):. 7/6/2022 [PMID: 35794479]
• FK Wong, M Selten, C Rosés-Nove, V Sreenivasa, N Pallas-Baz, E Serafeimid, A Hanusz-God, F Oozeer, R Edwards, O Marín Serotonergic regulation of bipolar cell survival in the developing cerebral cortex Cell Reports, 2022-07-05;40(1):111037. 2022-07-05 [PMID: 35793629] (IHC, Mouse)
• Laurent Jacob, Jose de Brito Neto, Stephanie Lenck, Celine Corcy, Farhat Benbelkacem, Luiz Henrique Geraldo et al. Conserved meningeal lymphatic drainage circuits in mice and humans Journal of Experimental Medicine 2022-08-01 [PMID: 35776089] (Immunohistochemistry, Mouse)
• AE Hall, SÖ Pohl, P Cammareri, S Aitken, NT Younger, M Raponi, CV Billard, AB Carrancio, A Bastem, P Freile, F Haward, IR Adams, JF Caceres, P Preyzner, A von Kriegs, MG Dunlop, FV Din, KB Myant RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer Nature Communications, 2022-05-19;13(1):2791. 2022-05-19 [PMID: 35589755] (IHC, Western Blot, Mouse)
• A Choudhury, ST Magill, CD Eaton, BC Prager, WC Chen, MA Cady, K Seo, CG Lucas, TJ Casey-Clyd, HN Vasudevan, SJ Liu, JE Villanueva, TC Lam, JK Pu, LF Li, GK Leung, DL Swaney, MY Zhang, JW Chan, Z Qiu, MV Martin, MS Susko, SE Braunstein, NAO Bush, JD Schulte, N Butowski, PK Sneed, MS Berger, NJ Krogan, A Perry, JJ Phillips, DA Solomon, JF Costello, MW McDermott, JN Rich, DR Raleigh Meningioma DNA methylation groups identify biological drivers and therapeutic vulnerabilities Nature Genetics, 2022-05-09;54(5):649-659. 2022-05-09 [PMID: 35534562] (IHC, Human, Xenograft)
• I Genescu, M Aníbal-Mar, V Kouskoff, N Chenouard, C Mailhes-Ha, H Cartonnet, L Lokmane, FM Rijli, G López-Bend, F Gambino, S Garel Dynamic interplay between thalamic activity and Cajal-Retzius cells regulates the wiring of cortical layer 1 Cell Reports, 2022-04-12;39(2):110667. 2022-04-12 [PMID: 35417707] (IHC, Mouse)
• Xu J, Xu L, Sui P et al. Excess neuropeptides in lung signal through endothelial cells to impair gas exchange Developmental cell 3/11/2022 [PMID: 35303432]
• Maxence Cornille, Stéphanie Moriceau, Roman H. Khonsari, Yann Heuzé, Léa Loisay, Valérie Boitez et al. FGFR3 overactivation in the brain is responsible for memory impairments in Crouzon syndrome mouse model J Exp Med 2022-03-07 [PMID: 35254402] (Immunohistochemistry, Mouse)
• MC Friess, I Kritikos, P Schineis, JD Medina-San, AO Gkountidi, A Vallone, EC Sigmund, C Schwitter, M Vranova, C Matti, J Arasa, C Saygili De, E Bovay, ST Proulx, M Tomura, A Rot, DF Legler, TV Petrova, C Halin Mechanosensitive ACKR4 scavenges CCR7 chemokines to facilitate T�cell de-adhesion and passive transport by flow in inflamed afferent lymphatics Cell Reports, 2022-02-01;38(5):110334. 2022-02-01 [PMID: 35108538] (IHC, Mouse)
• Peter Baluk, Donald M. McDonald Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts Methods in Molecular Biology 2022-09-06 [PMID: 35099733]
• Takahiro Wakizono, Hideyuki Nakashima, Tetsuro Yasui, Teppei Noda, Kei Aoyagi, Kanako Okada et al. Growth factors with valproic acid restore injury-impaired hearing by promoting neuronal regeneration JCI Insight 2021-11-22 [PMID: 34806649]
• Rafael S. Czepielewski, Emma C. Erlich, Emily J. Onufer, Shannon Young, Brian T. Saunders, Yong-Hyun Han et al. Ileitis-associated tertiary lymphoid organs originate at lymphatic valves and obstruct mesenteric lymph flow in response to tumor necrosis factor Immunity 2021-12-01 [PMID: 34788601]
• Wang T, Wang Z, de Fabritus L Et al. 1-deoxysphingolipids bind to COUP-TF to modulate lymphatic and cardiac cell development Developmental cell 11/22/2021 [PMID: 34762852]
• D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 2021-11-11;0(0):e12924. 2021-11-11 [PMID: 34762341] (IHC, Mouse)
• BR Thomson, P Liu, T Onay, J Du, SW Tompson, S Misener, RR Purohit, TL Young, J Jin, SE Quaggin Cellular crosstalk regulates the aqueous humor outflow pathway and provides new targets for glaucoma therapies Nature Communications, 2021-10-18;12(1):6072. 2021-10-18 [PMID: 34663817] (IHC, Mouse)
• NN Nawarawong, KR Thompson, SP Guerin, C Anasooya S, H Peng, K Nixon Reactive, Adult Neurogenesis From Increased Neural Progenitor Cell Proliferation Following Alcohol Dependence in Female Rats Frontiers in Neuroscience, 2021-09-14;15(0):689601. 2021-09-14 [PMID: 34594180] (IHC, Rat)
• E Redder, N Kirschnick, S Bobe, R Hägerling, NR Hansmeier, F Kiefer Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities PLoS ONE, 2021-09-20;16(9):e0249256. 2021-09-20 [PMID: 34543279] (IHC, Mouse)
• Masahiro Otsu, Zubair Ahmed, Daniel Fulton Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells Frontiers in Cell and Developmental Biology 2021-08-24 [PMID: 34504841] (Immunocytochemistry, Mouse)
• Tevye Jason Stachniak, Rahel Kastli, Olivia Hanley, Ali Özgür Argunsah, Elianne Grietje Theodora van der Valk, George Kanatouris et al. Postmitotic Prox1 Expression Controls the Final Specification of Cortical VIP Interneuron Subtypes The Journal of Neuroscience 2021-09-29 [PMID: 34380763]
• M Uemura, S Blankvoort, SSL Tok, L Yuan, LF Cobar, KK Lit, A Tashiro A neurogenic microenvironment defined by excitatory-inhibitory neuronal circuits in adult dentate gyrus Cell Reports, 2021-07-06;36(1):109324. 2021-07-06 [PMID: 34233196] (IHC, Mouse)
• Sara E. Billings, Nina M. Myers, Lee Quiruz, Alan G. Cheng Opposing effects of Wnt/ beta -catenin signaling on epithelial and mesenchymal cell fate in the developing cochlea Development 2021-06-01 [PMID: 34061174]
• Delayna Paulson, Rebecca Harms, Cody Ward, Mackenzie Latterell, Gregory J. Pazour, Darci M. Fink Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation Frontiers in Cell and Developmental Biology 2021-05-14 [PMID: 34055805]
• Wanshu Ma, Hyea Jin Gil, Xiaolei Liu, Lauren P. Diebold, Marc A. Morgan, Michael J. Oxendine-Burns et al. Mitochondrial respiration controls the Prox1-Vegfr3 feedback loop during lymphatic endothelial cell fate specification and maintenance Science Advances 2021-04-30 [PMID: 33931446]
• J Schaller, HM Hajjami, S Rusakiewic, K Ioannidou, N Piazzon, A Miles, D Golshayan, O Gaide, D Hohl, DE Speiser, K Schaeuble Mutually exclusive lymphangiogenesis or perineural infiltration in human skin squamous-cell carcinoma Oncotarget, 2021-03-30;12(7):638-648. 2021-03-30 [PMID: 33868585] (IHC, Human)
• G Limoni, S Murthy, D Jabaudon, A Dayer, M Niquille PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination Cell Reports, 2021-01-26;34(4):108644. 2021-01-26 [PMID: 33503438] (IHC, Mouse, Transgenic Mouse)
• I Tai-Nagara, Y Hasumi, D Kusumoto, H Hasumi, K Okabe, T Ando, F Matsuzaki, F Itoh, H Saya, C Liu, W Li, YS Mukouyama, W Marston Li, X Liu, M Hirashima, Y Suzuki, S Funasaki, Y Satou, M Furuya, M Baba, Y Kubota Blood and lymphatic systems are segregated by the FLCN tumor suppressor Nature Communications, 2020-12-09;11(1):6314. 2020-12-09 [PMID: 33298956] (IHC, Mouse)
• Motawe Z Y, Abdelmaboud S S et al. Involvement of Sigma Receptor-1 in Lymphatic Endothelial Barrier Integrity and Bioenergetic Regulation. Lymphat Res Biol 2024-06-15 [PMID: 33226886] (Simple Western, Human)
  
  Details:
  1:50
• A Rattner, CE Terrillion, C Jou, T Kleven, SF Hu, J Williams, Z Hou, M Aggarwal, S Mori, G Shin, LA Goff, MP Witter, M Pletnikov, AA Fenton, J Nathans Developmental, cellular, and behavioral phenotypes in a mouse model of congenital hypoplasia of the dentate gyrus Elife, 2020-10-21;9(0):. 2020-10-21 [PMID: 33084572] (IHC, Mouse)
• Boksik Cha, Yen-Chun Ho, Xin Geng, Md. Riaj Mahamud, Lijuan Chen, Yeunhee Kim et al. YAP and TAZ maintain PROX1 expression in the developing lymphatic and lymphovenous valves in response to VEGF-C signaling Development 2020-12-01 [PMID: 33060128]
• Thomson B, Grannonico M, Liu F et Al. Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma Transl Vis Sci Technol 8/21/2020 [PMID: 32818103]
• SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020-08-14;11(1):4102. 2020-08-14 [PMID: 32796823] (IHC, Mouse)
• E Kadosh, I Snir-Alkal, A Venkatacha, S May, A Lasry, E Elyada, A Zinger, M Shaham, G Vaalani, M Mernberger, T Stiewe, E Pikarsky, M Oren, Y Ben-Neriah The gut microbiome switches mutant p53 from tumour-suppressive to oncogenic Nature, 2020-07-29;0(0):. 2020-07-29 [PMID: 32728212] (IHC, Western Blot, Mouse, Transgenic Mouse)
• X Geng, K Yanagida, RG Akwii, D Choi, L Chen, Y Ho, B Cha, MR Mahamud, K Berman de, H Ichise, H Chen, J Wythe, CM Mikelis, T Hla, RS Srinivasan S1PR1 regulates the quiescence of lymphatic vessels by inhibiting laminar shear stress-dependent VEGF-C signaling JCI Insight, 2020-07-23;0(0):. 2020-07-23 [PMID: 32544090] (ICC, IHC, Human, Transgenic Mouse)
• Silvia Gramolelli, Endrit Elbasani, Krista Tuohinto, Veijo Nurminen, Thomas Günther, Riikka E. Kallinen et al. Oncogenic Herpesvirus Engages Endothelial Transcription Factors SOX18 and PROX1 to Increase Viral Genome Copies and Virus Production Cancer Research 2020-08-01 [PMID: 32518203]
• Pieter R Norden, Amélie Sabine, Ying Wang, Cansaran Saygili Demir, Ting Liu, Tatiana V Petrova et al. Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation eLife 2020-06-08 [PMID: 32510325]
• Menglan Xiang, Rubén Adrián Grosso, Akira Takeda, Junliang Pan, Tove Bekkhus, Kevin Brulois et al. A Single-Cell Transcriptional Roadmap of the Mouse and Human Lymph Node Lymphatic Vasculature Frontiers in Cardiovascular Medicine 2020-04-30 [PMID: 32426372]
• Goichi Akiyama, Sindhu Saraswathy, Thania Bogarin, Xiaojing Pan, Ernesto Barron, Tina T. Wong et al. Functional, structural, and molecular identification of lymphatic outflow from subconjunctival blebs Experimental Eye Research 2020-07-01 [PMID: 32387381]
• Y Yoshimatsu, S Kimuro, J Pauty, K Takagaki, S Nomiyama, A Inagawa, K Maeda, KA Podyma-Ino, K Kajiya, YT Matsunaga, T Watabe TGF-beta and TNF-alpha cooperatively induce mesenchymal transition of lymphatic endothelial cells via activation of Activin signals PLoS ONE, 2020-05-01;15(5):e0232356. 2020-05-01 [PMID: 32357159] (ICC, Human)
• A Roy, KJ Millen, RP Kapur Hippocampal granule cell dispersion: a non-specific finding in pediatric patients with no history of seizures Acta Neuropathol Commun, 2020-04-21;8(1):54. 2020-04-21 [PMID: 32317027] (IHC, Human)
• Fujimoto N, He Y, D'Addio M et al. Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes PLoS Biol. 4/1/2020 [PMID: 32251437]
• KL Betterman, DL Sutton, GA Secker, J Kazenwadel, A Oszmiana, L Lim, N Miura, L Sorokin, BM Hogan, ML Kahn, H McNeill, NL Harvey Atypical cadherin Fat4 orchestrates lymphatic endothelial cell polarity in response to flow J. Clin. Invest., 2020-06-01;0(0):. 2020-06-01 [PMID: 32182215] (IHC, Mouse)
• X Hu, Q Deng, L Ma, Q Li, Y Chen, Y Liao, F Zhou, C Zhang, L Shao, J Feng, T He, W Ning, Y Kong, Y Huo, A He, B Liu, J Zhang, R Adams, Y He, F Tang, X Bian, J Luo Meningeal lymphatic vessels regulate brain tumor drainage and immunity Cell Res., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094452] (IHC, Mouse)
• Eric Engelbrecht, Michel V Levesque, Liqun He, Michael Vanlandewijck, Anja Nitzsche, Hira Niazi et al. Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta eLife 2020-02-24 [PMID: 32091396]
• Lioux G, Liu X, Temi�o S et al. A Second Heart Field-Derived Vasculogenic Niche Contributes to Cardiac Lymphatics Developmental Cell 2020-02-01 [PMID: 31928974]
• Daniyal J Jafree, Dale Moulding, Maria Kolatsi-Joannou, Nuria Perretta Tejedor, Karen L Price, Natalie J Milmoe et al. Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease eLife 2019-12-06 [PMID: 31808745]
• M Rudzi?ska, M Mikula, KD Arczewska, E Gajda, S Sabali?ska, T St?pie?, J Ostrowski, B Czarnocka Transcription Factor Prospero Homeobox 1 (PROX1) as a Potential Angiogenic Regulator of Follicular Thyroid Cancer Dissemination Int J Mol Sci, 2019-11-10;20(22):. 2019-11-10 [PMID: 31717665] (Western Blot, Human)
• Dana Gancz, Brian C Raftrey, Gal Perlmoter, Rubén Marín-Juez, Jonathan Semo, Ryota L Matsuoka et al. Distinct origins and molecular mechanisms contribute to lymphatic formation during cardiac growth and regeneration eLife 2019-11-08 [PMID: 31702554]
• Chase M. Carver, Shayne D. Hastings, Mileah E. Cook, Mark S. Shapiro Functional responses of the hippocampus to hyperexcitability depend on directed, neuron‐specific KCNQ2 K + channel plasticity Hippocampus 2020-05-01 [PMID: 31621989]
• Benjamin R Thomson, Isabel A Carota, Tomokazu Souma, Saily Soman, Dietmar Vestweber, Susan E Quaggin Targeting the vascular-specific phosphatase PTPRB protects against retinal ganglion cell loss in a pre-clinical model of glaucoma eLife 2019-10-17 [PMID: 31621585]
• Md. Riaj Mahamud, Xin Geng, Yen-Chun Ho, Boksik Cha, Yuenhee Kim, Jing Ma et al. GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126 Development 2019-11-01 [PMID: 31582413] (Immunohistochemistry, Western Blot, Human, Transgenic Mouse)
• A Kasurinen, S Gramolelli, J Hagström, A Laitinen, A Kokkola, Y Miki, K Lehti, M Yashiro, PM Ojala, C Böckelman, C Haglund High tissue MMP14 expression predicts worse survival in gastric cancer, particularly with a low PROX1 Cancer Med, 2019-09-27;0(0):. 2019-09-27 [PMID: 31560170] (IHC-P, Human)
• SY Yoon, LC Dieterich, S Karaman, ST Proulx, SB Bachmann, C Sciaroni, M Detmar An important role of cutaneous lymphatic vessels in coordinating and promoting anagen hair follicle growth PLoS ONE, 2019-07-25;14(7):e0220341. 2019-07-25 [PMID: 31344105] (IHC, Mouse)
• Alice E. Denton, Edward J. Carr, Lukasz P. Magiera, Andrew J.B. Watts, Douglas T. Fearon Embryonic FAP+ lymphoid tissue organizer cells generate the reticular network of adult lymph nodes Journal of Experimental Medicine 2019-10-07 [PMID: 31324739]
• X Ding, H Wang, X Qian, X Han, L Yang, Y Cao, Q Wang, J Yang Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity Cell Rep, 2019-06-25;27(13):3799-3807.e3. 2019-06-25 [PMID: 31242414] (IHC, Mouse)
• SF Sorrells, MF Paredes, D Velmeshev, V Herranz-Pé, K Sandoval, S Mayer, EF Chang, R Insausti, AR Kriegstein, JL Rubenstein, J Manuel Gar, EJ Huang, A Alvarez-Bu Immature excitatory neurons develop during adolescence in the human amygdala Nat Commun, 2019-06-21;10(1):2748. 2019-06-21 [PMID: 31227709] (IHC, Human)
• TA Norman, AC Gower, F Chen, A Fine Transcriptional landscape of pulmonary lymphatic endothelial cells during fetal gestation PLoS ONE, 2019-05-13;14(5):e0216795. 2019-05-13 [PMID: 31083674] (IHC-P, Mouse)
• M Rudzi?ska, M Grzanka, A Stachurska, M Mikula, K Paczkowska, T St?pie?, A Paziewska, J Ostrowski, B Czarnocka Molecular Signature of Prospero Homeobox 1 (PROX1) in Follicular Thyroid Carcinoma Cells Int J Mol Sci, 2019-05-05;20(9):. 2019-05-05 [PMID: 31060342] (IHC-P, Human)
• RMJ Genga, EM Kernfeld, KM Parsi, TJ Parsons, MJ Ziller, R Maehr Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development Cell Rep, 2019-04-16;27(3):708-718.e10. 2019-04-16 [PMID: 30995470] (ICC, Human)
• Maria A.S. Broggi, Lea Maillat, Cristina C. Clement, Natacha Bordry, Patricia Corthésy, Aymeric Auger et al. Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients Journal of Experimental Medicine 2019-05-06 [PMID: 30975896]
• JV Pluvinage, MS Haney, BAH Smith, J Sun, T Iram, L Bonanno, L Li, DP Lee, DW Morgens, AC Yang, SR Shuken, D Gate, M Scott, P Khatri, J Luo, CR Bertozzi, MC Bassik, T Wyss-Coray CD22 blockade restores homeostatic microglial phagocytosis in ageing brains Nature, 2019-04-03;0(0):. 2019-04-03 [PMID: 30944478] (IHC, Mouse)
• CM Carver, MS Shapiro Gq-coupled muscarinic receptor enhancement of KCNQ2/3 channels and activation of TRPC channels in multimodal control of excitability in dentate gyrus granule cells J. Neurosci., 2018-12-28;0(0):. 2018-12-28 [PMID: 30593498] (IHC, Mouse)
• Sofia Urner, Lara Planas‐Paz, Laura Sophie Hilger, Carina Henning, Anna Branopolski, Molly Kelly‐Goss et al. Identification of ILK as a critical regulator of VEGFR 3 signalling and lymphatic vascular growth The EMBO Journal 2019-01-15 [PMID: 30518533]
• T Vuorio, E Ylä-Herttu, JP Laakkonen, S Laidinen, T Liimataine, S Ylä-Herttu Downregulation of VEGFR3 signaling alters cardiac lymphatic vessel organization and leads to a higher mortality after acute myocardial infarction Sci Rep, 2018-11-12;8(1):16709. 2018-11-12 [PMID: 30420641] (IHC, Mouse)
• B Cha, X Geng, MR Mahamud, JY Zhang, L Chen, W Kim, EH Jho, Y Kim, D Choi, JB Dixon, H Chen, YK Hong, L Olson, TH Kim, BJ Merrill, MJ Davis, RS Srinivasan Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/?-Catenin Signaling Cell Rep, 2018-10-16;25(3):571-584.e5. 2018-10-16 [PMID: 30332639] (IHC, Western Blot, Human, Mouse)
• René Hägerling, Esther Hoppe, Cathrin Dierkes, Martin Stehling, Taija Makinen, Stefan Butz et al. Distinct roles of VE ‐cadherin for development and maintenance of specific lymph vessel beds The EMBO Journal 2018-11-15 [PMID: 30297530]
• Peter Baluk, Donald M. McDonald Imaging Lymphatics in Mouse Lungs Methods in Molecular Biology 2019-04-29 [PMID: 30242759]
• J Högström, S Heino, P Kallio, M Lähde, VM Leppänen, D Balboa, Z Wiener, K Alitalo Transcription factor PROX1 suppresses Notch pathway activation via the nucleosome remodeling and deacetylase complex in colorectal cancer stem-like cells Cancer Res., 2018-08-28;0(0):. 2018-08-28 [PMID: 30154153] (Flow Cytometry, Mouse)
• KM Goudarzi, JA Espinoza, M Guo, J Bartek, M Nistér, MS Lindström, D Hägerstran Reduced expression of PROX1 transitions glioblastoma cells into a mesenchymal gene expression subtype Cancer Res., 2018-08-22;0(0):. 2018-08-22 [PMID: 30135192] (Western Blot, Human)
• Duncan I. Mackie, Fuad Al Mutairi, Reema B. Davis, Daniel O. Kechele, Natalie R. Nielsen, Joshua C. Snyder et al. hCALCRL mutation causes autosomal recessive nonimmune hydrops fetalis with lymphatic dysplasia Journal of Experimental Medicine 2018-09-03 [PMID: 30115739]
• S Gramolelli, J Cheng, I Martinez-C, M Vähä-Koske, E Elbasani, E Kaivanto, V Rantanen, K Tuohinto, S Hautaniemi, M Bower, C Haglund, K Alitalo, T Mäkinen, TV Petrova, K Lehti, PM Ojala PROX1 is a transcriptional regulator of MMP14 Sci Rep, 2018-06-22;8(1):9531. 2018-06-22 [PMID: 29934628] (ChIP, Western Blot, Mouse)
• M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program Nat Commun, 2018-04-17;9(1):1511. 2018-04-17 [PMID: 29666442] (IHC, Mouse)
• M Niquille, G Limoni, F Markopoulo, C Cadilhac, J Prados, A Holtmaat, A Dayer Neurogliaform cortical interneurons derive from cells in the preoptic area Elife, 2018-03-20;7(0):. 2018-03-20 [PMID: 29557780] (IHC-Fr, Mouse)
• M Xu, X Han, R Liu, Y Li, C Qi, Z Yang, C Zhao, J Gao PDK1 Deficit Impairs the Development of the Dentate Gyrus in Mice Cereb. Cortex, 2019-03-01;0(0):. 2019-03-01 [PMID: 29420689] (IHC, Mouse)
• M Rudzinska, JK Ledwon, D Gawel, J Sikorska, B Czarnocka The role of prospero homeobox 1 (PROX1) expression in follicular thyroid carcinoma cells Oncotarget, 2017-12-12;8(69):114136-114155. 2017-12-12 [PMID: 29371975] (Western Blot, Human)
• S Rhee, JI Chung, DA King, G D'amato, DT Paik, A Duan, A Chang, D Nagelberg, B Sharma, Y Jeong, M Diehn, JC Wu, AJ Morrison, K Red-Horse Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease Nat Commun, 2018-01-25;9(1):368. 2018-01-25 [PMID: 29371594] (IHC, Mouse)
• Hoshino A, Ratnapriya R, Brooks MJ et al. Molecular Anatomy of the Developing Human Retina. Dev. Cell 12/18/2017 [PMID: 29233477]
• Salli Antila, Sinem Karaman, Harri Nurmi, Mikko Airavaara, Merja H. Voutilainen, Thomas Mathivet et al. Development and plasticity of meningeal lymphatic vessels Journal of Experimental Medicine 2017-12-04 [PMID: 29141865]
• James Hughes, Ruby Dawson, Melinda Tea, Dale McAninch, Sandra Piltz, Dominique Jackson et al. Knockout of the epilepsy gene Depdc5 in mice causes severe embryonic dysmorphology with hyperactivity of mTORC1 signalling Scientific Reports 2017-10-03 [PMID: 28974734]
• J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm&#039;s canal integrity and induces glaucoma J. Clin. Invest., 2017-09-18;0(0):. 2017-09-18 [PMID: 28920924] (ICC, IHC, Western Blot, Human, Mouse)
• YC Lin, N Ohbayashi, T Hongu, N Katagiri, Y Funakoshi, H Lee, Y Kanaho Arf6 in lymphatic endothelial cells regulates lymphangiogenesis by controlling directional cell migration Sci Rep, 2017-09-12;7(1):11431. 2017-09-12 [PMID: 28900118] (IHC, Mouse)
• A Laitinen, C Böckelman, J Hagström, A Kokkola, P Kallio, C Haglund High PROX1 expression in gastric cancer predicts better survival PLoS ONE, 2017-08-30;12(8):e0183868. 2017-08-30 [PMID: 28854215] (IHC-P, Human)
• R Hägerling, D Drees, A Scherzinge, C Dierkes, S Martin-Alm, S Butz, K Gordon, M Schäfers, K Hinrichs, P Ostergaard, D Vestweber, T Goerge, S Mansour, X Jiang, PS Mortimer, F Kiefer VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations JCI Insight, 2017-08-17;2(16):. 2017-08-17 [PMID: 28814672] (IHC-Fr, Human)
• Oliver Lyons, Prakash Saha, Christopher Seet, Adam Kuchta, Andrew Arnold, Steven Grover et al. Human venous valve disease caused by mutations in FOXC2 and GJC2 Journal of Experimental Medicine 2017-08-07 [PMID: 28724617]
• Y Mimura-Yam, H Shinohara, T Kashiwagi, T Sato, S Shioda, T Seki Dynamics and function of CXCR4 in formation of the granule cell layer during hippocampal development Sci Rep, 2017-07-17;7(1):5647. 2017-07-17 [PMID: 28717168] (IHC, Mouse)
• E Jung, D Gardner, D Choi, E Park, Y Jin Seong, S Yang, J Castorena-, A Louveau, Z Zhou, GK Lee, DP Perrault, S Lee, M Johnson, G Daghlian, M Lee, Y Jin Hong, Y Kato, J Kipnis, MJ Davis, AK Wong, YK Hong Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm&#039;s Canal Reporter Rat Sci Rep, 2017-07-17;7(1):5577. 2017-07-17 [PMID: 28717161] (IHC, Rat)
• SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1 Sci Rep, 2017-07-07;7(1):4916. 2017-07-07 [PMID: 28687807] (ICC, Human)
• KS Yan, O Gevaert, GXY Zheng, B Anchang, CS Probert, KA Larkin, PS Davies, ZF Cheng, JS Kaddis, A Han, K Roelf, RI Calderon, E Cynn, X Hu, K Mandleywal, J Wilhelmy, SM Grimes, DC Corney, SC Boutet, JM Terry, P Belgrader, SB Ziraldo, TS Mikkelsen, F Wang, RJ von Furste, NR Smith, P Chandrakes, R May, MAS Chrissy, R Jain, CA Cartwright, JC Niland, YK Hong, J Carrington, DT Breault, J Epstein, CW Houchen, JP Lynch, MG Martin, SK Plevritis, C Curtis, HP Ji, L Li, SJ Henning, MH Wong, CJ Kuo Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity Cell Stem Cell, 2017-07-06;21(1):78-90.e6. 2017-07-06 [PMID: 28686870] (IHC-Fr, IHC-P, Mouse)
• LD Volk-Drape, KL Hall, AC Wilber, S Ran Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4 PLoS ONE, 2017-06-09;12(6):e0179257. 2017-06-09 [PMID: 28598999] (ICC, Human)
• J Matsushita, S Inagaki, T Nishie, T Sakasai, J Tanaka, C Watanabe, KI Mizutani, Y Miwa, K Matsumoto, K Takara, H Naito, H Kidoya, N Takakura, T Nagai, S Takahashi, M Ema Fluorescence and Bioluminescence Imaging of Angiogenesis in Flk1-Nano-lantern Transgenic Mice Sci Rep, 2017-04-20;7(0):46597. 2017-04-20 [PMID: 28425492] (IHC, Mouse)
• JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray Human umbilical cord plasma proteins revitalize hippocampal function in aged mice Nature, 2017-04-19;544(7651):488-492. 2017-04-19 [PMID: 28424512] (IHC-Fr, Mouse)
• JJ Teoh, T Iwano, M Kunii, N Atik, E Avriyanti, SI Yoshimura, K Moriwaki, A Harada BIG1 is required for the survival of deep layer neurons, neuronal polarity, and the formation of axonal tracts between the thalamus and neocortex in developing brain PLoS ONE, 2017-04-17;12(4):e0175888. 2017-04-17 [PMID: 28414797] (IHC, Mouse)
• K Bermudez-H, YL Lu, J Moretto, S Jain, JJ LaFrancois, AM Duffy, HE Scharfman Hilar granule cells of the mouse dentate gyrus: effects of age, septotemporal location, strain, and selective deletion of the proapoptotic gene BAX Brain Struct Funct, 2017-03-17;0(0):. 3/17/2017 [PMID: 28314928]
• S Frazer, J Prados, M Niquille, C Cadilhac, F Markopoulo, L Gomez, U Tomasello, L Telley, A Holtmaat, D Jabaudon, A Dayer Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing Nat Commun, 2017-01-30;8(0):14219. 2017-01-30 [PMID: 28134272] (IHC-Fr, Mouse)
• Koji Nishimura, Teppei Noda, Alain Dabdoub Dynamic Expression of Sox2, Gata3, and Prox1 during Primary Auditory Neuron Development in the Mammalian Cochlea PLOS ONE 2017-01-24 [PMID: 28118374]
• M Malinverno, M Corada, L Ferrarini, L Formicola, G Marazzi, D Sassoon, E Dejana Peg3/PW1 is a marker of a subset of vessel associated endothelial progenitors Stem Cells, 2017-02-05;0(0):. 2017-02-05 [PMID: 28090691] (IHC, Mouse)
• Cédric Francius, María Hidalgo-Figueroa, Stéphanie Debrulle, Barbara Pelosi, Vincent Rucchin, Kara Ronellenfitch et al. Vsx1 Transiently Defines an Early Intermediate V2 Interneuron Precursor Compartment in the Mouse Developing Spinal Cord Frontiers in Molecular Neuroscience 2016-12-26 [PMID: 28082864]
• Gene-expression profiling of different arms of lymphatic vasculature identifies candidates for manipulation of cell traffic Proc Natl Acad Sci USA, 2016-09-06;113(38):10643-10648. 2016-09-06 [PMID: 27601677] (IHC, Mouse)
• Lymphatic vessels regulate immune microenvironments in human and murine melanoma J Clin Invest, 2016-08-15;0(0):. 2016-08-15 [PMID: 27525437] (IHC-P, Human)
• Boksik Cha Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves Genes Dev, 2016-06-16;30(12):1454-69. 2016-06-16 [PMID: 27313318] (IHC-Fr, Western Blot, Human, Mouse)
• Foxc1 and Foxc2 deletion causes abnormal lymphangiogenesis and correlates with ERK hyperactivation J Clin Invest, 2016-05-23;0(0):. 2016-05-23 [PMID: 27214551] (Flow Cytometry, IHC-P, Mouse)
• CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity J Clin Invest, 2016-05-03;0(0):. 2016-05-03 [PMID: 27140400] (IHC-P, Mouse)
• Geng R, Noda T, Mulvaney J, Lin V, Edge A, Dabdoub A Comprehensive Expression of Wnt Signaling Pathway Genes during Development and Maturation of the Mouse Cochlea. PLoS ONE, 2016-02-09;11(2):e0148339. 2016-02-09 [PMID: 26859490] (IHC, Mouse)
• Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development. Dev Biol, 2015-11-02;409(1):218-33. 2015-11-02 [PMID: 26542011] (IHC, Mouse)
• Goichi Miyoshi, Allison Young, Timothy Petros, Theofanis Karayannis, Melissa McKenzie Chang, Alfonso Lavado et al. Prox1 Regulates the Subtype-Specific Development of Caudal Ganglionic Eminence-Derived GABAergic Cortical Interneurons The Journal of Neuroscience 2015-09-16 [PMID: 26377473] (Immunohistochemistry, Mouse)
• Aspelund A, Antila S, Proulx S, Karlsen T, Karaman S, Detmar M, Wiig H, Alitalo K A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules. J Exp Med, 2015-06-15;212(7):991-9. 2015-06-15 [PMID: 26077718] (IHC, Mouse)
• Johanna Liebl, Siwei Zhang, Markus Moser, Yan Agalarov, Cansaran Saygili Demir, Bianca Hager et al. Cdk5 controls lymphatic vessel development and function by phosphorylation of Foxc2 Nature Communications 2015-06-01 [PMID: 26027726] (Immunohistochemistry, Western Blot, Mouse)
• Klotz L, Norman S, Vieira JM et al. Cardiac lymphatics are heterogeneous in origin and respond to injury. Nature. 5/20/2015 [PMID: 25992544]
• Bianchi R, Teijeira A, Proulx S, Christiansen A, Seidel C, Rulicke T, Makinen T, Hagerling R, Halin C, Detmar M A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research. PLoS ONE, 2015-04-07;10(4):e0122976. 2015-04-07 [PMID: 25849579] (IHC-Fr, Mouse)
• Xianghu Qu, Bin Zhou, H. Scott Scott Baldwin Tie1 is required for lymphatic valve and collecting vessel development Developmental Biology 2015-03-01 [PMID: 25576926] (Immunohistochemistry, Transgenic Mouse)
• Heidi I. Chen, Bikram Sharma, Brynn N. Akerberg, Harri J. Numi, Riikka Kivelä, Pipsa Saharinen et al. The sinus venosus contributes to coronary vasculature through VEGFC-stimulated angiogenesis Development 2014-12-01 [PMID: 25377552]
• Heidi I. Chen, Aruna Poduri, Harri Numi, Riikka Kivela, Pipsa Saharinen, Andrew S. McKay et al. VEGF-C and aortic cardiomyocytes guide coronary artery stem development Journal of Clinical Investigation 2014-11-03 [PMID: 25271623]
• Van't Hull E, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye J, Doucey M Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer. Oncoimmunology, 2014-06-25;3(0):e29080. 2014-06-25 [PMID: 25101222] (ICC, Human)
• Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K The Schlemm&#039;s canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel. J Clin Invest, 2014-07-25;124(9):3975-86. 2014-07-25 [PMID: 25061878] (IHC, IHC-P, Human, Zebrafish)
• Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G Lymphatic regulator PROX1 determines Schlemm&#039;s canal integrity and identity. J Clin Invest, 2014-07-25;124(9):3960-74. 2014-07-25 [PMID: 25061877] (IHC, Mouse)
• Zheng W, Nurmi H, Appak S, Sabine A, Bovay E, Korhonen E, Orsenigo F, Lohela M, D'Amico G, Holopainen T, Leow C, Dejana E, Petrova T, Augustin H, Alitalo K Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions. Genes Dev, 2014-07-15;28(14):1592-603. 2014-07-15 [PMID: 25030698] (IHC-Fr, Mouse)
• Fatima A, Culver A, Culver F, Liu T, Dietz W, Thomson B, Hadjantonakis A, Quaggin S, Kume T Murine Notch1 is required for lymphatic vascular morphogenesis during development. Dev Dyn, 2014-04-17;243(7):957-64. 2014-04-17 [PMID: 24659232] (IHC, Mouse)
• Brzezinski J, Uoon Park K, Reh T Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence. Dev Biol, 2013-10-12;384(2):194-204. 2013-10-12 [PMID: 24125957] (IHC-Fr, Mouse)
• Sugiyama T, Osumi N, Katsuyama Y The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages. Dev Dyn, 2013-10-07;242(12):1442-53. 2013-10-07 [PMID: 24038449] (IHC, Mouse)
• Francius C, Harris A, Rucchin V et al. Identification of Multiple Subsets of Ventral Interneurons and Differential Distribution along the Rostrocaudal Axis of the Developing Spinal Cord. PLoS One. 8/15/2013 [PMID: 23967072]
• Justin A. McClain, Stephanie A. Morris, S. Alexander Marshall, Kimberly Nixon Ectopic hippocampal neurogenesis in adolescent male rats following alcohol dependence Addiction Biology 2014-07-01 [PMID: 23844726]
• Sandrine Levet, Delphine Ciais, Galina Merdzhanova, Christine Mallet, Teresa A. Zimmers, Se-Jin Lee et al. Bone morphogenetic protein 9 (BMP9) controls lymphatic vessel maturation and valve formation Blood 2013-07-25 [PMID: 23741013]
• Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. EMBO J, 2013-01-08;32(5):629-44. 2013-01-08 [PMID: 23299940] (IHC, Mouse)
• Aberrant mural cell recruitment to lymphatic vessels and impaired lymphatic drainage in a murine model of pulmonary fibrosis. Blood, 2012-04-30;119(24):5931-42. 2012-04-30 [PMID: 22547584] (ICC, IHC-P, Mouse)
• Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival. Blood, 2012-02-28;119(24):5850-8. 2012-02-28 [PMID: 22374700] (IHC, Mouse)
• Lara Planas-Paz, Boris Strilić, Axel Goedecke, Georg Breier, Reinhard Fässler, Eckhard Lammert Mechanoinduction of lymph vessel expansion The EMBO Journal 2012-02-15 [PMID: 22157817] (Immunohistochemistry, Mouse)
• Skog M, Bono P, Lundin M, Lundin J, Louhimo J, Linder N, Petrova TV, Andersson LC, Joensuu H, Alitalo K, Haglund CH Expression and prognostic value of transcription factor PROX1 in colorectal cancer. Br. J. Cancer, 2011-10-04;105(9):1346-51. 2011-10-04 [PMID: 21970873] (IHC-P, Human)
• Elyada E, Pribluda A, Goldstein RE, Morgenstern Y, Brachya G, Cojocaru G, Snir-Alkalay I, Burstain I, Haffner-Krausz R, Jung S, Wiener Z, Alitalo K, Oren M, Pikarsky E, Ben-Neriah Y CKIalpha ablation highlights a critical role for p53 in invasiveness control. Nature, 2011-02-17;470(7334):409-13. 2011-02-17 [PMID: 21331045] (IHC-P, Western Blot, Mouse)
• Xiaoren Chen, Tapan P. Patel, Vladimir I. Simirskii, Melinda K. Duncan PCNA interacts with Prox1 and represses its transcriptional activity Mol Vis 2008-11-17 [PMID: 19023449]
• Philipp M, Fralish GB, Meloni AR, Chen W, MacInnes AW, Barak LS, Caron MG Smoothened signaling in vertebrates is facilitated by a G protein-coupled receptor kinase. Mol. Biol. Cell, 2008-09-24;19(12):5478-89. 2008-09-24 [PMID: 18815277] (IHC, Zebrafish)
• Heckman CA, Holopainen T, Wirzenius M, Keskitalo S, Jeltsch M, Yla-Herttuala S, Wedge SR, Jurgensmeier JM, Alitalo K The tyrosine kinase inhibitor cediranib blocks ligand-induced vascular endothelial growth factor receptor-3 activity and lymphangiogenesis. Cancer Res., 2008-06-15;68(12):4754-62. 2008-06-15 [PMID: 18559522] (ICC, Human)

Reviews for Prox1 Antibody (AF2727) (4)

Average Rating: 4.8

(Based on 4 reviews)

We have 4 reviews tested in 4 species: Human, Mouse, Other, Rabbit.

reviewed by: Verified Customer IHC-Fr Rabbit 07/07/2020

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: brain hippocampus section,Rabbit Brain dentate gyrus section
• Species: Rabbit
• Lot: VIY0218061

reviewed by: Wei-Sheng Chen WB Human 07/13/2015

Summary

• Application: Western Blot
• Sample Tested: primary human lymphatic endothelial cells
• Species: Human

reviewed by: Verified Customer IF Mouse 01/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23299940
• Species: Mouse

reviewed by: Verified Customer IHC-P Other 01/07/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22115316
• Species: Other

Bioinformatics

• Gene Symbol: PROX1
• Uniprot:
  • Q92786()