Prox1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by ...read more
Prox1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) at 15 µg/mL for 3 hours at room ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Prox1 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Prox1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Prox1
Pro2-Gln259
Accession # Q92786
Specificity
Detects human Prox1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PROX1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF2727 in the following applications:

Publications
Read Publications using
AF2727 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prox1 Antibody [Unconjugated]

  • Homeobox prospero-like protein PROX1
  • prospero homeobox 1
  • prospero homeobox protein 1
  • prospero-related homeobox 1
  • Prox1
  • PROX-1

Background

Prox1, the homologue of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence homology with mouse and rat Prox1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prox1 Antibody (AF2727)(41)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 14 applications: Flow, ICC, IHC, IHC Frozen, IHC - Not specified, IHC Fresh, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC-Fr, IHC-OCT embedded, WB, immunohistochemistry.


Filter By Application
Flow
(1)
ICC
(5)
IHC
(7)
IHC Frozen
(2)
IHC - Not specified
(1)
IHC Fresh
(2)
IHC Frozen
(3)
IHC Not Specified
(3)
IHC OCT-embedded
(3)
IHC Paraffin-embedded
(5)
IHC-Fr
(2)
IHC-OCT embedded
(1)
WB
(4)
immunohistochemistry
(1)
All Applications
Filter By Species
Human
(12)
Mouse
(26)
Rat
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using AF2727 Applications Species
M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program Nat Commun, 2018;9(1):1511. 2018 [PMID: 29666442] (IHC,, Mouse) IHC Mouse
M Niquille, G Limoni, F Markopoulo, C Cadilhac, J Prados, A Holtmaat, A Dayer Neurogliaform cortical interneurons derive from cells in the preoptic area Elife, 2018;7(0):. 2018 [PMID: 29557780] (IHC-Fr,, Mouse) IHC-Fr Mouse
M Rudzinska, JK Ledwon, D Gawel, J Sikorska, B Czarnocka The role of prospero homeobox 1 (PROX1) expression in follicular thyroid carcinoma cells Oncotarget, 2017;8(69):114136-114155. 2017 [PMID: 29371975] (WB,, Human) WB Human
S Rhee, JI Chung, DA King, G D'amato, DT Paik, A Duan, A Chang, D Nagelberg, B Sharma, Y Jeong, M Diehn, JC Wu, AJ Morrison, K Red-Horse Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease Nat Commun, 2018;9(1):368. 2018 [PMID: 29371594] (IHC,, Mouse) IHC Mouse
J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28920924] (WB,, Human) WB Human
YC Lin, N Ohbayashi, T Hongu, N Katagiri, Y Funakoshi, H Lee, Y Kanaho Arf6 in lymphatic endothelial cells regulates lymphangiogenesis by controlling directional cell migration Sci Rep, 2017;7(1):11431. 2017 [PMID: 28900118] (IHC,, Mouse) IHC Mouse
A Laitinen, C Böckelman, J Hagström, A Kokkola, P Kallio, C Haglund High PROX1 expression in gastric cancer predicts better survival PLoS ONE, 2017;12(8):e0183868. 2017 [PMID: 28854215] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
R Hägerling, D Drees, A Scherzinge, C Dierkes, S Martin-Alm, S Butz, K Gordon, M Schäfers, K Hinrichs, P Ostergaard, D Vestweber, T Goerge, S Mansour, X Jiang, PS Mortimer, F Kiefer VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations JCI Insight, 2017;2(16):. 2017 [PMID: 28814672] (IHC-Fr,, Human) IHC-Fr Human
Y Mimura-Yam, H Shinohara, T Kashiwagi, T Sato, S Shioda, T Seki Dynamics and function of CXCR4 in formation of the granule cell layer during hippocampal development Sci Rep, 2017;7(1):5647. 2017 [PMID: 28717168] (IHC,, Mouse) IHC Mouse
E Jung, D Gardner, D Choi, E Park, Y Jin Seong, S Yang, J Castorena-, A Louveau, Z Zhou, GK Lee, DP Perrault, S Lee, M Johnson, G Daghlian, M Lee, Y Jin Hong, Y Kato, J Kipnis, MJ Davis, AK Wong, YK Hong Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm's Canal Reporter Rat Sci Rep, 2017;7(1):5577. 2017 [PMID: 28717161] (IHC,, Rat) IHC Rat
Show All 41 Publications.

Reviews for Prox1 Antibody (AF2727) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Other.

Reviews using AF2727:
Filter by Applications
WB
(1)
IF
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Prox1 AF2727
Enlarge
reviewed by:
Wei-Sheng Chen
WB Human 07/13/2015
View

Summary

ApplicationWestern Blot
Sample Testedprimary human lymphatic endothelial cells
SpeciesHuman

Blocking

Blocking Details0.5x Odyssey's blocking buffer, RT, 30 min

Primary Anitbody

Dilution Ratio1,000 dilution in 0.5x Odyssey's blocking buffer, 4C, overnight.

Secondary Antibody

Secondary DescriptionDonkey anti-goat IgG, 10,000 dilution in 0.5x Odyssey's blocking buffer, RT, 45 min.

Details

Detection NotesOdyssey® CLx Infrared Imaging System
 
reviewed by:
Anonymous
IF Mouse 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23299940
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23299940

Details

Detection NotesSee PMID 23299940

Comments

CommentsPublished: See PMID 23299940
 
reviewed by:
Anonymous
IHC-P Other 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22115316
SpeciesOther

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Prox1 Antibody (AF2727) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Prox1 Antibody (AF2727)

Discover related pathways, diseases and genes to Prox1 Antibody (AF2727). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Prox1 Antibody (AF2727)

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Blogs on Prox1.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Wei-Sheng Chen
07/13/2015
Application: WB
Species: Human

 
Anonymous
01/07/2015
Application: IF
Species: Mouse

 
Anonymous
01/07/2015
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol PROX1
Entrez
Uniprot