Hemoglobin A2 Antibody

Images

 
Western Blot: Hemoglobin A2 Antibody [NBP3-05569] - Western blot analysis of extracts of mouse liver, using Hemoglobin A2 antibody (NBP3-05569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunohistochemistry: Hemoglobin A2 Antibody [NBP3-05569] - Immunofluorescence analysis of Mouse placenta using Hemoglobin A2 antibody (NBP3-05569) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry: Hemoglobin A2 Antibody [NBP3-05569] - Immunofluorescence analysis of Human placenta using Hemoglobin A2 antibody (NBP3-05569) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry: Hemoglobin A2 Antibody [NBP3-05569] - Immunofluorescence analysis of Rat placenta using Hemoglobin A2 antibody (NBP3-05569) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Hemoglobin A2 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human Hemoglobin A2 (NP_000508.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HBA2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Western Blot 1:500 - 1:2000
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Hemoglobin A2 Antibody

  • alpha globin
  • alpha-2 globin
  • alpha-globin
  • Hemoglobin alpha chain
  • hemoglobin subunit alpha
  • hemoglobin, alpha 2

Background

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta -pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) codingsequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but theydiffer significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA,which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains toconstitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemiasresult from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alphathalassemias have also been reported. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Hemoglobin A2 Antibody (NBP3-05569)

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Calculators

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Bioinformatics

Gene Symbol HBA2