HB-EGF Antibody [Unconjugated]

Images

 
Recombinant Human HB-EGF (Catalog # 259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB-EGF (20 ...read more
HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑259‑NA) overnight at 4 ...read more
HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Dynamic expression and processing of Tgf-alpha and Hb-egf during multistep pancreatic neuroendocrine tumorigenesis (PNET). (A) Relative expression of Egf family members in cDNAs prepared from total RNA extracts of ...read more
Biological Strategies: BHLHE40-knockdown reduced hypoxia-induced expression of a panel of cytokines and growth factors. a Heatmaps of cytokines and growth factors whose hypoxia-induced expression (1% O2 at 6 h ...read more
Biological Strategies: Egfr activity contributes to RT2 tumor growth and angiogenic switching. (A) Relative expression quantified by real-time quantitative PCR of Erbb family members in cDNAs derived from total ...read more
Hb-egf contributes to the angiogenic switch and neovascularization of PNET tumors. (A) Comparison of the average number of hemorrhagic angiogenic islets in wild-type (wt) and Hb-egf mutant (Hb) 9-week-old RT2 mice. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
   

Biological Strategies

     

Order Details

HB-EGF Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HB-EGF (R&D Systems, Catalog # 259-HE)
Asp63-Leu148
Accession # Q99075
Specificity
Detects human HB-EGF in ELISAs and Western blots. In direct ELISAs, less than 1% cross reactivity with recombinant mouse HB-EGF is observed. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HBEGF
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.1-0.6 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human HB-EGF Antibody (Catalog # AF-259-NA)
ELISA Detection:Human HB-EGF Biotinylated Antibody (Catalog # BAF259)
Standard: Recombinant Human HB-EGF (Catalog # 259-HE)
Publications
Read Publications using
AF-259-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HB-EGF Antibody [Unconjugated]

  • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor)
  • Dtr
  • DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor)
  • Dts
  • DTSF
  • HBEGF
  • HB-EGF
  • Hegfl
  • heparin-binding EGF-like growth factor
  • heparin-binding epidermal growth factor
  • proheparin-binding EGF-like growth factor

Background

HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogenous O-glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis.

HB-EGF is a member of the EGF family of mitogens which also include transforming growth factor-alpha (TGF-alpha ), amphiregulin (AR), rat schwanoma-derived growth factor (SDGF), vaccinia growth factor (VGF), and the various ligands for the Her2/ErbB2/neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha , and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HB-EGF Antibody (AF-259-NA)(58)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate - Chlorocebus pygerythrus (Vervet Monkey).

We have publications tested in 9 applications: Cell Culture, ELISA Capture, Flow Cytometry, ICC, IHC, IHC-P, Immunohistochemistry, Neutralization, Western Blot.


Filter By Application
Cell Culture
(1)
ELISA Capture
(1)
Flow Cytometry
(1)
ICC
(3)
IHC
(7)
IHC-P
(6)
Immunohistochemistry
(1)
Neutralization
(16)
Western Blot
(6)
All Applications
Filter By Species
Human
(30)
Mouse
(8)
Rat
(4)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using AF-259-NA Applications Species
Won, KJ;Lee, R;Choi, SH;Kim, JH;Hwang, SH;Nah, SY; Gintonin-Induced Wound-Healing-Related Responses Involve Epidermal-Growth-Factor-like Effects in Keratinocytes International journal of molecular sciences 2023-09-14 [PMID: 37762395] (Western Blot, Human) Western Blot Human
Okamoto, M;Sasai, M;Kuratani, A;Okuzaki, D;Arai, M;Wing, JB;Sakaguchi, S;Yamamoto, M; A genetic method specifically delineates Th1-type Treg cells and their roles in tumor immunity Cell reports 2023-07-25 [PMID: 37440410] (IHC, Mouse) IHC Mouse
Rafael Soares Godoy, Nicholas D Cober, David P Cook, Emma McCourt, Yupu Deng, Liyuan Wang et al. Single-cell transcriptomic atlas of lung microvascular regeneration after targeted endothelial cell ablation eLife 4/20/2023 [PMID: 37078698]
Q Yu, I Gamayun, P Wartenberg, Q Zhang, S Qiao, S Kusumakshi, S Candlish, V Götz, S Wen, D Das, A Wyatt, V Wahl, F Ectors, K Kattler, D Yildiz, V Prevot, M Schwaninge, G Ternier, P Giacobini, P Ciofi, TD Müller, U Boehm Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis Nature Communications, 2023-03-22;14(1):1588. 2023-03-22 [PMID: 36949050] (IHC, Mouse) IHC Mouse
Nancy G. Casanova, Vivian Reyes-Hernon, Taylor Gregory, Belinda Sun, Tadeo Bermudez, Matthew K. Hufford et al. Biochemical and genomic identification of novel biomarkers in progressive sarcoidosis: HBEGF, eNAMPT, and ANG-2 Frontiers in Medicine 10/25/2022 [PMID: 36388923]
C Zhang, LK Vincelette, F Reimann, SD Liberles A brainstem circuit for nausea suppression Cell Reports, 2022-06-14;39(11):110953. 2022-06-14 [PMID: 35705049] (IHC, Mouse) IHC Mouse
Haixiao Wang, Lingjuan He, Yan Li, Wenjuan Pu, Shaohua Zhang, Ximeng Han et al. Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo Journal of Biological Chemistry 6/1/2022 [PMID: 35461809]
Barbara Schreier, Virginie Dubourg, Stefanie Hübschmann, Sindy Rabe, Sigrid Mildenberger, Michael Gekle Synergy of epidermal growth factor (EGFR) and angiotensin II (AT1R) receptor determines composition and temporal pattern of transcriptome variation Cellular and Molecular Life Sciences 1/1/2022 [PMID: 34921637]
Jun Watanabe, Yuki Takayanagi, Masahide Yoshida, Tatsuya Hattori, Michiko Saito, Kenji Kohno et al. Conditional ablation of vasopressin‐synthesizing neurons in transgenic rats Journal of Neuroendocrinology 12/1/2021 [PMID: 34748241]
M Fujita, M Sasada, M Eguchi, T Iyoda, S Okuyama, T Osawa, K Tsuzuranuk, M Sakamoto, Y Hagihara, M Matsumura, S Osada, H Kodama, Y Higami, F Fukai Induction of cellular senescence in fibroblasts through beta1-integrin activation by tenascin-C-derived peptide and its protumor effect American journal of cancer research, 2021-09-15;11(9):4364-4379. 2021-09-15 [PMID: 34659892] (Neutralization, Human) Neutralization Human
Show All 58 Publications.

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Bioinformatics

Gene Symbol HBEGF
Uniprot