Recombinant Human TGF-alpha Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TGF-alpha Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is 0.1-0.4 ng/mL.
Source
E. coli-derived human TGF-alpha protein
Val40-Ala89
Accession #
N-terminal Sequence
Val40
Protein/Peptide Type
Recombinant Proteins
Gene
TGFA
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
239-A in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-alpha Protein, CF

  • protransforming growth factor alpha
  • TFGA
  • TGFA
  • TGFalpha
  • TGF-alpha
  • transforming growth factor, alpha
  • transforming growth factor-alpha

Background

TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha  shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.

TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene.

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Publications for TGF-alpha (239-A)(38)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Hamster, N/A.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), ELISA Developmet, In Vivo, Stimulation.


Filter By Application
Bioassay
(31)
ELISA (Standard)
(1)
ELISA Developmet
(1)
In Vivo
(4)
Stimulation
(1)
All Applications
Filter By Species
Human
(23)
Mouse
(9)
Rat
(3)
Chicken
(1)
Hamster
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using 239-A Applications Species
J Peterson, B Lin, CM Barrios-Ca, DB Herrick, EH Holbrook, W Jang, JH Coleman, JE Schwob Activating a Reserve Neural Stem Cell Population In�Vitro Enables Engraftment and Multipotency after Transplantation Stem Cell Reports, 2019;0(0):. 2019 [PMID: 30930245] (Stimulation, Human) Stimulation Human
Y Li, H Li, Y Duan, X Cai, D You, F Zhou, C Yang, X Tuo, Z Liu Blockage of TGF-? Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells Biomed Res Int, 2019;2019(0):8231267. 2019 [PMID: 30906781] (Bioassay, Human) Bioassay Human
N Priego, L Zhu, C Monteiro, M Mulders, D Wasilewski, W Bindeman, L Doglio, L Martínez, E Martínez-S, SRY Cajal, D Megías, E Hernández-, C Blanco-Apa, L Martínez, E Zarzuela, J Muñoz, C Fustero-To, E Pineiro, A Hernández-, L Bertero, V Poli, M Sánchez-Ma, JA Menendez, R Soffietti, J Bosch-Barr, M Valiente STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis Nat. Med., 2018;0(0):. 2018 [PMID: 29892069] (Bioassay, Human) Bioassay Human
LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko Epiregulin and EGFR interactions are involved in pain processing J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28783046] (In Vivo, Mouse) In Vivo Mouse
TS Lau, LK Chan, EC Wong, CW Hui, K Sneddon, TH Cheung, SF Yim, JH Lee, CS Yeung, TK Chung, J Kwong A loop of cancer-stroma-cancer interaction promotes peritoneal metastasis of ovarian cancer via TNF?-TGF?-EGFR Oncogene, 2017;0(0):. 2017 [PMID: 28166193] (Bioassay, Human) Bioassay Human
EGFR signaling is critical for maintaining the superficial layer of articular cartilage and preventing osteoarthritis initiation Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):14360-14365. 2016 [PMID: 27911782] (Bioassay, Mouse) Bioassay Mouse
R Matsushima, H Toyoda, T Nagasawa, E Yasuda, N Chiba, S Okuda, A Maeda, Y Kaneoka, T Kumada, O Kozawa Phosphorylated Heat Shock Protein 20 (HSPB6) Regulates Transforming Growth Factor-?-Induced Migration and Invasion of Hepatocellular Carcinoma Cells PLoS ONE, 2016;11(4):e0151907. 2016 [PMID: 27046040] (Bioassay, Human) Bioassay Human
Negative regulation of the hepatic fibrogenic response by suppressor of cytokine signaling 1 Cytokine, 2016;82(0):58-69. 2016 [PMID: 26748724] (Bioassay, Mouse) Bioassay Mouse
Zhang C, Spevak W, Zhang Y, Burton E, Ma Y, Habets G, Zhang J, Lin J, Ewing T, Matusow B, Tsang G, Marimuthu A, Cho H, Wu G, Wang W, Fong D, Nguyen H, Shi S, Womack P, Nespi M, Shellooe R, Carias H, Powell B, Light E, Sanftner L, Walters J, Tsai J, West B, Visor G, Rezaei H, Lin P, Nolop K, Ibrahim P, Hirth P, Bollag G RAF inhibitors that evade paradoxical MAPK pathway activation. Nature, 2015;526(7574):583-6. 2015 [PMID: 26466569] (Bioassay, Mouse) Bioassay Mouse
Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences. J Invest Dermatol, 2015;135(7):1771-80. 2015 [PMID: 25739051] (Bioassay, Human) Bioassay Human
Show All 38 Publications.

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Bioinformatics

Gene Symbol TGFA
Uniprot