Recombinant Human TGF-alpha Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TGF-alpha Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is 0.1-0.4 ng/mL.
Source
E. coli-derived human TGF-alpha protein
Val40-Ala89
Accession #
N-terminal Sequence
Val40
Protein/Peptide Type
Recombinant Proteins
Gene
TGFA
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
239-A in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-alpha Protein, CF

  • protransforming growth factor alpha
  • TFGA
  • TGFA
  • TGFalpha
  • TGF-alpha
  • transforming growth factor, alpha
  • transforming growth factor-alpha

Background

TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha  shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.

TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene.

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Publications for TGF-alpha (239-A)(48)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Hamster, N/A, Porcine.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), ELISA Developmet, In Vivo, Stimulation.


Filter By Application
Bioassay
(40)
Cell Culture
(1)
ELISA (Standard)
(1)
ELISA Developmet
(1)
In Vivo
(4)
Stimulation
(1)
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Filter By Species
Human
(32)
Mouse
(9)
Rat
(3)
Chicken
(1)
Hamster
(1)
N/A
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using 239-A Applications Species
R Matsushima, N Yamada, Y Hattori, Y Hosokawa, J Tachi, T Hori, O Kozawa SERMs (selective estrogen receptor modulator), acting as estrogen receptor beta agonists in hepatocellular carcinoma cells, inhibit the transforming growth factor-alpha-induced migration via specific inhibition of AKT signaling pathway PLoS ONE, 2022;17(1):e0262485. 2022 [PMID: 35007301] (Bioassay, Human) Bioassay Human
IT Stancil, JE Michalski, D Davis-Hall, HW Chu, JA Park, CM Magin, IV Yang, BJ Smith, E Dobrinskik, DA Schwartz Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional Nature Communications, 2021;12(1):4566. 2021 [PMID: 34315881] (Bioassay, Human) Bioassay Human
J Sugita, K Fujiu, Y Nakayama, T Matsubara, J Matsuda, T Oshima, Y Liu, Y Maru, E Hasumi, T Kojima, H Seno, K Asano, A Ishijima, N Tomii, M Yamazaki, F Kudo, I Sakuma, R Nagai, I Manabe, I Komuro Cardiac macrophages prevent sudden death during heart stress Nature Communications, 2021;12(1):1910. 2021 [PMID: 33771995] (Bioassay, Human) Bioassay Human
K Nomi, R Hayashi, Y Ishikawa, Y Kobayashi, T Katayama, AJ Quantock, K Nishida Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs Cell Reports, 2021;34(5):108715. 2021 [PMID: 33535050] (Bioassay, Human) Bioassay Human
S Feng, J Wu, WL Qiu, L Yang, X Deng, Y Zhou, Y Chen, X Li, L Yu, H Li, ZR Xu, Y Xiao, X Ren, L Zhang, C Wang, Z Sun, J Wang, X Ding, Y Chen, P Gadue, G Pan, M Ogawa, S Ogawa, J Na, P Zhang, L Hui, H Yin, L Chen, CR Xu, X Cheng Large-scale Generation of Functional and Transplantable Hepatocytes and Cholangiocytes from Human Endoderm Stem Cells Cell Reports, 2020;33(10):108455. 2020 [PMID: 33296648] (Bioassay, Human) Bioassay Human
K Kobayashi, R Matsushima, N Yamada, S Migita, T Hioki, D Mizutani, O Kozawa Heat shock protein 70 positively regulates transforming growth factor-&amp;alpha-induced hepatocellular carcinoma cell migration via the AKT signaling pathway Heliyon, 2020;6(9):e05002. 2020 [PMID: 33005803] (Bioassay, Human) Bioassay Human
S Liu, Y Wang, Y Han, W Xia, L Zhang, S Xu, H Ju, X Zhang, G Ren, L Liu, W Ye, Z Zhang, J Hu EREG-driven oncogenesis of Head and Neck Squamous Cell Carcinoma exhibits higher sensitivity to Erlotinib therapy Theranostics, 2020;10(23):10589-10605. 2020 [PMID: 32929368] (Bioassay, Human) Bioassay Human
N Yamada, R Matsushima, O Kozawa Quercetin suppresses the migration of hepatocellular carcinoma cells stimulated by hepatocyte growth factor or transforming growth factor-&amp;alpha: Attenuation of AKT signaling pathway Arch. Biochem. Biophys., 2020;682(0):108296. 2020 [PMID: 32032576] (Bioassay, Human) Bioassay Human
L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking Cell Rep, 2019;29(7):2067-2077.e6. 2019 [PMID: 31722218] (Cell Culture, Human) Cell Culture Human
K Yoshioka, K Uchikura, T Suda, S Matoba Production of piglets from in�vitro-produced blastocysts by ultrasound-guided ovum pick-up from live donors Theriogenology, 2019;141(0):113-119. 2019 [PMID: 31536860] (Bioassay, Porcine) Bioassay Porcine
Show All 48 Publications.

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Bioinformatics

Gene Symbol TGFA
Uniprot