RFX1 Antibody - BSA Free

Western Blot: RFX1 Antibody [NBP2-82031] - Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody - BSA Free [NBP2-82031] - Immunocytochemistry of RFX1 in Hela cells with RFX1 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody - BSA Free [NBP2-82031] - Immunofluorescence of RFX1 in Hela cells with RFX1 antibody at 20 ug/mL.Green: RFX1 Antibody Blue: DAPI staining

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody - BSA Free [NBP2-82031] - Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RFX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RFX1 Antibody - BSA Free (NBP2-82031) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1. The immunogen is located within the last 50 amino acids of RFX1. Amino Acid Squence: AGGESPALGPETLEPPAK
• Specificity: RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFX1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse: (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RFX1 Antibody - BSA Free

• EFC
• EF-C
• Enhancer factor C
• MHC class II regulatory factor RFX
• MHC class II regulatory factor RFX1
• Regulatory factor X 1
• regulatory factor X, 1 (influences HLA class II expression)
• RFX
• trans-acting regulatory factor 1
• Transcription factor RFX1

Background

RFX1, also known as MHC class II regulatory factor RFX1, is a 105 kDa 979 amino acid protein found in the nucleus. The gene plays a significant role in the gene expression of MHC class II. Current research on RFX1 is being conducted in relation to hepatitis, leukemia, neuroblastoma , sickle cell anemia and adenocarcinoma. RFX1 has been linked to the methlation, immune response, spermatogenesis and localization pathways where it interacts with TADA3, SMAD1, HDAC1, ADD1 and SMAD9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFX1