RFX1 Antibody

Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: RFX1 Antibody [NBP1-52654] - Section stained with anti-RFX1 antibody. RFX1 belongs to Cluster 4. Spermatids were stained in brown. These distribution patterns were mostly consisted to that of trancripts. (Abbreviation: SPT=spermatid).Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26753906/) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Immunoprecipitation: RFX1 Antibody [NBP1-52654] - Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for IP at 6 ug per reaction. RFX1 was also immunoprecipitated by another rabbit anti-RFX1 antibody. For blotting immunoprecipitated RFX1, NBP1-52654 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RFX1 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 929 and 979 of human Regulatory Factor X, 1 using the numbering given in entry NP_002909.4 (GeneID 5989).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel and ReliaBLOT(R) Reagents. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RFX1 Antibody

• EFC
• EF-C
• Enhancer factor C
• MHC class II regulatory factor RFX
• MHC class II regulatory factor RFX1
• Regulatory factor X 1
• regulatory factor X, 1 (influences HLA class II expression)
• RFX
• trans-acting regulatory factor 1
• Transcription factor RFX1

Background

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RFX1 Antibody (NBP1-52654)(4)

Publications using NBP1-52654

• Herranz-PErez V, Nakatani J, Ishii M et al. Ependymoma associated protein Zfta is expressed in immature ependymal cells but is not essential for ependymal development in mice Research Square 2021-12-13 [PMID: 35087169] (IF/IHC, Human)
• Zhang H, Qin Z, Yue X Et al. Proteome-wide profiling of transcriptional machinery on accessible chromatin with biotinylated transposons Science advances 2021-10-22 [PMID: 34678055] (Chip Cytometry)
• Julien L, Chassagne J, Peccate C et al. RFX1 and RFX3 Transcription Factors Interact with the D Sequence of Adeno-Associated Virus Inverted Terminal Repeat and Regulate AAV Transduction Sci Rep 2018-01-09 [PMID: 29317724] (WB, Human)
• Zhu Z, Li C, Yang S et al. Dynamics of the Transcriptome during Human Spermatogenesis: Predicting the Potential Key Genes Regulating Male Gametes Generation. Sci Rep. 2016-01-12 [PMID: 26753906] (IHC-P, Human)

Bioinformatics

• Gene Symbol: RFX1