RFX1 Antibody - BSA Free

Western Blot: RFX1 Antibody [NBP1-52653] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RFX1 antibody used for IP at 6 mcg/mg lysate. RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies NBP1-52652 and NBP1-52654, which recognize other epitopes.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RFX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RFX1 Antibody - BSA Free (NBP1-52653) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 200 and 250 of human Regulatory Factor X, 1 using the numbering given in entry NP_002909.4 (GeneID 5989).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 mcg/mg
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RFX1 Antibody - BSA Free

• EFC
• EF-C
• Enhancer factor C
• MHC class II regulatory factor RFX
• MHC class II regulatory factor RFX1
• Regulatory factor X 1
• regulatory factor X, 1 (influences HLA class II expression)
• RFX
• trans-acting regulatory factor 1
• Transcription factor RFX1

Background

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFX1