Reelin Antibody (CL5324) - BSA Free

Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of rat cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.

Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of neurons in layer 1.

Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of human tonsil shows no positivity in lymphoid cells as expected (negative control).

Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of mouse cerebral cortex shows positivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.

Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of mouse cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.

Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of mouse embryo E11 shows strong positivity in cells in the outer layer of developing cortical plate.

Immunohistochemistry-Paraffin: Reelin Antibody (CL5324) [NBP2-61414] - Staining of rat cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL5324
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Reelin Antibody (CL5324) - BSA Free Summary

• Description: Novus Biologicals Mouse Reelin Antibody (CL5324) - BSA Free (NBP2-61414) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P78509, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RELN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Reelin Recombinant Protein Antigen (NBP2-61414PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Reelin Antibody (CL5324) - BSA Free

• EC 3.4.21
• EC 3.4.21.-
• Reeler
• Reelin
• RELN
• RL
• RLPRO1598

Background

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RELN