Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
This ApoER2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~106 kDa representing the membrane form of ApoER2. A larger ~130 kDa band may also be seen, representing the glycosylated form of ApoER2. Use in SDS PAGE reported in scientific literature (PMID 27810638).
Read Publications using NB100-2216 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for Apolipoprotein E R2/ApoE R2 Antibody
Apolipoprotein E R2
Apolipoprotein E receptor 2
low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
low-density lipoprotein receptor-related protein 8
ApoER2 (apolipoprotein E receptor 2 or LDL receptor related protein 8) is a member of LDL receptor (LDLR) family and it acts as a cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. ApoER2 facilitates extracellular RELN signal transduction to intracellular signaling processes, mainly through binding with DAB1 on its cytoplasmic tail. RELN acts via both VLDLR and ApoER2 to regulate tyrosine phosphorylation mediated DAB1 activation and microtubule function in neuronal cells. ApoER2 possess higher affinity for RELN compared to VLDLR and is therefore, a key player in Reelin pathway that governs forebrain's neuronal layering during prenatal brain development. ApoER2 associates with ER resident receptor-associated protein (RAP) as well as with dimers of beta 2-glycoprotein I. In the vasculature, ApoER2 has been suggested to implicate in suppressing platelet aggregation. Moreover, ApoER2 is highly expressed in epididymis (initial segment), where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), which are required for sperm maturation. ApoER2 also function as an endocytic receptor and defective ApoER2 have been linked to myocardial infarction type 1 (MCI1).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Ding J, Bowes R C. Synaptic Expression of Apoer2 in the Plexiform Layers of Mouse Retina. 2008 Annual Meeting of the Association for Research in Vision Ophthalmology (ARVO); Fort Lauderdale, FL; USA. 27-Apr-08.
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