DAB1 Antibody

Western Blot: DAB1 Antibody [NBP2-16095] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dab1 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: DAB1 Antibody [NBP2-16095] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DAB1 protein stained by DAB1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-16095] - Mouse duodenum. DAB1 antibody [N1N3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Ch
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DAB1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human DAB1. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Chicken (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DAB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for DAB1 Antibody

• disabled (Drosophila) homolog 1
• disabled homolog 1 (Drosophila)
• disabled homolog 1

Background

The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAB1 Antibody (NBP2-16095)(1)

Publication using NBP2-16095

• Slater E, Wilke L, Bohm L Et Al. Combinations of Low-Frequency Genetic Variants Might Predispose to Familial Pancreatic Cancer Journal of Personalized Medicine 2021-07-02 [PMID: 34357098]

Bioinformatics

• Gene Symbol: DAB1
• Uniprot:
  • O75553(Human)