Reelin Antibody (CL5324) - Azide and BSA Free

Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of neurons in layer 1.

Staining of mouse embryo E11 shows strong positivity in cells in the outer layer of developing cortical plate.

Staining of human tonsil shows no positivity in lymphoid cells as expected (negative control).

Staining of rat cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.

Staining of mouse cerebral cortex shows positivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.

Staining of mouse cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.

Staining of rat cerebral cortex shows immunoreactivity in the neuropil of layer 1, as well as cytoplasmic positivity in a subset of neurons in various layers.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL5324
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Reelin Antibody (CL5324) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P78509, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RELN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Reelin Antibody (CL5324) - Azide and BSA Free

• EC 3.4.21
• EC 3.4.21.-
• Reeler
• Reelin
• RELN
• RL
• RLPRO1598

Background

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RELN