Recombinant Mouse TLR6 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse TLR6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized peptidoglycan at 10 µg/mL (100 µL/well) can bind Recombinant Mouse TLR6 Fc Chimera with a linear range of 0.003-2 µg/mL.
• Source: Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse TLR6 protein
  

  Mouse TLR6 (Leu43-Asp594) Accession # BAA78632	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: BAA78632
• N-terminal Sequence: Leu43
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tlr6
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 89.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TLR6 Fc Chimera Protein, CF

• CD286 antigen
• CD286
• TLR6
• toll-like receptor 6

Background

The Toll‑like family of molecules serve as pattern recognition receptors that activate the immune system in response to microbial pathogens. Toll‑like receptors (TLRs) are type I transmembrane proteins in either the plasma membrane or the membranes of endosomal structures. TLRs contain a large number of leucine-rich repeats (LRRs) in their extracellular domains (ECD) and a cytoplasmic tail with one signal transducing Toll/IL‑1 receptor (TIR) domain (1, 2). Mature mouse TLR6 (approximately 115 kDa) consists of a 557 amino acid (aa) ECD, a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain (3). Within the ECD, mouse TLR6 shares 73% and 86% aa sequence identity with human and rat TLR6, respectively. TLR6 is expressed on the surface of macrophages, monocytes, neutrophils, dermal endothelial cells, and alveolar epithelial cells in ligand‑independent association with TLR2 (4‑7). TLR2 also associates with TLR1, creating a functional receptor complex with specificity for related microbial ligands (4, 8, 9). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycans, and glycosylphosphatidylinositols (4‑6, 10‑13). TLR6 plays an important role in the control of Th17 responses and IL‑23 production during allergic inflammation and fungal‑induced asthma (7).

1. Mills, K.H. (2011) Nat. Rev. Immunol. 11:807.
2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
3. Takeuchi, O. et al. (1999) Gene 231:59.
4. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
5. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
6. Bulut, Y. et al. (2001) J. Immunol. 167:987.
7. Moreira, A.P. et al. (2011) J. Clin. Invest. 121:4420.
8. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
9. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
10. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
11. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
13. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.

Bioinformatics

• Gene Symbol: Tlr6
• Uniprot:
  • BAA78632()