Recombinant Mouse Noggin Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Catalog# & Formulation Size Price

Recombinant Mouse Noggin Fc Chimera Protein, CF Summary

Additional Information
A New rm Noggin is Now Available! It has ~2 fold better activity, lower endotoxin specifications, and no tag!
Details of Functionality
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.06-0.3 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).
Source
Mouse myeloma cell line, NS0-derived mouse Noggin protein
Mouse Noggin
(Leu20-Cys232)
Accession # U79163
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Nog
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
50 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55-60 kDa, reducing conditions
Publications
Read Publications using
719-NG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Noggin Fc Chimera Protein, CF

  • NOG
  • Noggin
  • SYM1
  • symphalangism 1 (proximal)
  • synostoses (multiple) syndrome 1
  • SYNS1
  • SYNS1A

Background

Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse noggin shares 99% and 83% aa sequence identity with human and Xenopus noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

  1. Smith, W.C. and R.M. Harland (1992) Cell 70:829.
  2. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  3. Brunet, L.J. et al. (1998) Science 280:1455.

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719-NG
Species: Mu
Applications: Bioactivity

Publications for Noggin (719-NG)(16)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Xenopus.

We have publications tested in 3 applications: Bioassay, In Vivo, In vivo.


Filter By Application
Bioassay
(8)
In Vivo
(6)
In vivo
(1)
All Applications
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Human
(2)
Mouse
(4)
Rat
(1)
Bovine
(1)
Chicken
(4)
Xenopus
(4)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 719-NG Applications Species
BA Boswell, A Korol, JA West-Mays, LS Musil Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract Mol. Biol. Cell, 2017;0(0):. 2017 [PMID: 28209733] (Bioassay, Chicken) Bioassay Chicken
Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation Sci Rep, 2016;6(0):29200. 2016 [PMID: 27383650] (Bioassay, Human) Bioassay Human
Sengle G, Carlberg V, Tufa S, Charbonneau N, Smaldone S, Carlson E, Ramirez F, Keene D, Sakai L Abnormal Activation of BMP Signaling Causes Myopathy in Fbn2 Null Mice. PLoS Genet, 2015;11(6):e1005340. 2015 [PMID: 26114882] (Bioassay, Mouse) Bioassay Mouse
Ueki Y, Wilken M, Cox K, Chipman L, Bermingham-McDonogh O, Reh T A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation. Development, 2015;142(3):533-43. 2015 [PMID: 25605781] (Mouse) Mouse
Yamamoto-Shiraishi Y, Kuroiwa A Wnt and BMP signaling cooperate with Hox in the control of Six2 expression in limb tendon precursor., 2013;0(0):. 2013 [PMID: 23499659] (In vivo, Mouse) In vivo Mouse
Boswell BA, Overbeek PA, Musil LS Essential role of BMPs in FGF-induced secondary lens fiber differentiation. Dev. Biol., 2008;324(2):202-12. 2008 [PMID: 18848538] (Bioassay, Chicken) Bioassay Chicken
Boswell BA, Lein PJ, Musil LS Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells. Mol. Biol. Cell, 2008;19(6):2631-41. 2008 [PMID: 18400943] (Bioassay, Chicken) Bioassay Chicken
Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm. Stem Cells, 2008;26(4):874-85. 2008 [PMID: 18238854] (Bioassay, Human) Bioassay Human
Yao Y, Zebboudj AF, Torres A, Shao E, Bostrom K Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells. Cardiovasc. Res., 2007;74(2):279-89. 2007 [PMID: 17074310] (Bioassay, Bovine) Bioassay Bovine
Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 2006;133(19):3787-96. 2006 [PMID: 16943279] (Bioassay, Mouse) Bioassay Mouse
Show All 16 Publications.

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Bioinformatics

Gene Symbol Nog
Uniprot