Recombinant Mouse Noggin (aa 28-232) Protein, CF

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (314-BP).

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Noggin (aa 28-232) Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.04-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-GP).
• Source: Mouse myeloma cell line, NS0-derived mouse Noggin protein
  Gln28-Cys232
• Accession #: P97466
• N-terminal Sequence: No results obtained: Gln28 predicted, sequencing might be blocked
• Protein/Peptide Type: Recombinant Proteins
• Gene: Nog
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 23 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25-35 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Noggin (aa 28-232) Protein, CF

• NOG
• Noggin
• SYM1
• symphalangism 1 (proximal)
• synostoses (multiple) syndrome 1
• SYNS1
• SYNS1A

Background

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N‑terminal acidic region, a central basic
heparin‑binding segment and a C‑terminal cysteine‑knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of
heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature mouse Noggin shares 99%, 100%, 98%, 97% and 87% aa sequence identity with human, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP‑4 with high affinity and others such as BMP‑7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine‑knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons (6, 9-13). Noggin also appears to maintain adult stem cell populations in vivo, for example, maintaining neural stem cells within the hippocampus (13).

1. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
2. Groppe, J. et al. (2002) Nature 420:636.
3. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
4. Brunet, L. J. et al. (1998) Science 280:1455.
5. McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
6. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
7. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
8. Gong, Y. et al. (1999) Nat. Genet. 21:302.
9. Xu, R.-H. et al. (2005) Nat. Methods 2:185.
10. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.
11. Chaturvedi, G. et al. (2009) Cell Prolif. 42:425.
12. Chiba, S. et al. (2008) Stem Cells 26:2810.
13. Bonaguidi, M.A. et al. (2008) J. Neurosci. 28:9194.

Bioinformatics

• Gene Symbol: Nog
• Uniprot:
  • P97466()