Recombinant Human TSG-6 Protein, CF


1 μg/lane of Recombinant Human TSG-6 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 40 kDa.
Recombinant Human TSG-6 (Catalog # 2104-TS) binds hyaluronan in a functional ELISA. When Recombinant Human TSG-6 is present at 0.5 µg/mL, the concentration of biotinylated hyaluronan that produces 50% of the optimal more

Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human TSG-6 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human TSG-6 is present at 0.5 μg/well, the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is found to be approximately 4-30 ng/mL.
Mouse myeloma cell line, NS0-derived human TSG-6 protein
Trp18-Leu277, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
30.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
40 kDa, reducing conditions
Read Publications using
2104-TS in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TSG-6 Protein, CF

  • Hyaluronate-binding protein
  • TNF alpha-induced protein 6
  • TNF-stimulated gene 6 protein
  • TSG6
  • TSG-6
  • TSG-6tumor necrosis factor alpha-inducible protein 6
  • TSG6tumor necrosis factor-inducible gene 6 protein
  • Tumor necrosis factor alpha-induced protein 6
  • tumor necrosis factor, alpha-induced protein 6
  • tumor necrosis factor-stimulated gene-6 protein


TSG-6 (TNF-stimulated gene 6; also TNFIP6) is a secreted, 35-39 kDa group A member of the LINK‑Module superfamily of proteins (1 - 4). Human TSG‑6 is synthesized as a 277 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 260 aa mature region (5, 6). The mature region shows an N-terminal LINK module (aa 36-129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135-247). Link modules are alpha -helical, beta -sheet structures that bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature human TSG-6 shares 94% aa identity with both mouse and canine TSG-6. Cells reported to express TSG-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral, plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or link, between the individual subunits of extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80-85 kDa HC subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a cation‑dependent catalyst for the removal, transfer, and subsequent covalent linkage of HC 1/2 to surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). The disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  6. Lee, T.H. et al. (1992) J. Cell Biol. 116:545.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.

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Publications for TSG-6 (2104-TS)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), ELISA Standard, In Vivo.

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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using 2104-TS Applications Species
TJ Bartosh, JH Ylostalo Efficacy of 3D Culture Priming is Maintained in Human Mesenchymal Stem Cells after Extensive Expansion of the Cells Cells, 2019;8(9):. 2019 [PMID: 31491901] (ELISA Standard, Human) ELISA Standard Human
T Muraguchi, D Nanba, EK Nishimura, T Tashiro IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 31122679] (Bioassay, Human) Bioassay Human
Y Chen, X Tang, P Li, Y Zhou, T Xue, J Liu, C Yu Bone Marrow Derived Mesenchymal Stromal Cells Ameliorate Ischemia/Reperfusion Injury-Induced Acute Kidney Injury in Rats via Secreting Tumor Necrosis Factor-Inducible Gene 6 Protein Biomed Res Int, 2019;2019(0):9845709. 2019 [PMID: 30984789] (Bioassay, Rat) Bioassay Rat
K Ni, A Gill, D Cao, K Koike, KS Schweitzer, S Garantziot, I Petrache Intravascular heavy chain-modification of hyaluronan during endotoxic shock Biochem Biophys Rep, 2019;17(0):114-121. 2019 [PMID: 30623115] (Bioassay, Mouse) Bioassay Mouse
YS Lv, R Gao, QM Lin, T Jiang, Q Chen, SX Tang, HP Mao, HL Zhou, LS Cao The role of TSG-6 and uroplakin III in bladder pain syndrome/ interstitial cystitis in rats and humans Iran J Basic Med Sci, 2017;20(11):1242-1249. 2017 [PMID: 29299202] (In Vivo, Rat) In Vivo Rat
L Kui, GC Chan, PP Lee TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells Mediators Inflamm., 2017;2017(0):7462945. 2017 [PMID: 28367002] (Bioassay, Human) Bioassay Human
Intravenous hMSCs Ameliorate Acute Pancreatitis in Mice via Secretion of Tumor Necrosis Factor-? Stimulated Gene/Protein 6 Sci Rep, 2016;6(0):38438. 2016 [PMID: 27917949] (In Vivo, Mouse) In Vivo Mouse
TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158. 2016 [PMID: 27911817] (In Vivo, Mouse) In Vivo Mouse
Tonia L Vincent Acute Molecular Changes in Synovial Fluid Following Human Knee Injury: Association With Early Clinical Outcomes Arthritis & rheumatology (Hoboken, N.J.), 2016;68(9):2129-40. 2016 [PMID: 26991527] (ELISA (Standard), Human) ELISA (Standard) Human
Kim D, Choi H, Nishida H, Oh J, Gregory C, Lee R, Yu J, Watanabe J, An S, Bartosh T, Prockop D Scalable Production of a Multifunctional Protein (TSG-6) That Aggregates with Itself and the CHO Cells That Synthesize It. PLoS ONE, 2016;11(1):e0147553. 2016 [PMID: 26793973] (In Vivo, Mouse) In Vivo Mouse
Show All 17 Publications.

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Gene Symbol TNFAIP6