Recombinant Human TLR7 Fc Chimera Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human TLR7 Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human TLR7 protein
  

  Human TLR7 (Ala27-Asn839) Accession # Q9NYK1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9NYK1
• N-terminal Sequence: Ala27
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 122-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TLR7 Fc Chimera Protein, CF

• TLR7
• toll-like receptor 7

Background

TLR7 (Toll-like receptor 7) is a member of the Toll-like receptor family of innate immune receptors. It is mainly expressed in lung, placenta and spleen and belongs to a new sub-family of the TLRs comprising of TLR7, TLR8, an TLR9 (1, 2). Human TLR7 cDNA encodes a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 813 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. Human TLR7 is located in the endosomes and involved in the sequence-dependent sensing of single-stranded RNA, thereby detecting bacterial or viral nucleic acids (3, 4, 5).

1. Du, X. et al. (2000) Eur. Cytokine Netw. 11:362.
2. Chuang, T.H. and R.J. Ulevitch (2000) Eur. Cytokine Netw. 11:372.
3. Hornung, V. et al. (2002) J. Immunol. 168:4531.
4. Melchjorsen, J. et al. (2005) J. Virol. 79:12944.
5. Gantier, M.P. et al. (2008) J. Immunol. 180:2117.

Publications for TLR7 (9567-TR)(2)

Publications using 9567-TR

• Metthew Lam, LK;Oatman, E;Eckart, KA;Klingensmith, NJ;Flowers, E;Sayegh, L;Yuen, J;Clements, RL;Meyer, NJ;Jurado, KA;Vaughan, AE;Eisenbarth, SC;Mangalmurti, NS; Human red blood cells express the RNA sensor TLR7 Scientific reports 2024-07-09 [PMID: 38982195] (Bioassay, Human)
• Crawford, JD;Wang, H;Trejo-Zambrano, D;Cimbro, R;Talbot, CC;Thomas, MA;Curran, AM;Girgis, AA;Schroeder, JT;Fava, A;Goldman, DW;Petri, M;Rosen, A;Antiochos, B;Darrah, E; The XIST lncRNA is a sex-specific reservoir of TLR7 ligands in SLE JCI insight 2023-09-21 [PMID: 37733447] (Bioassay, N/A)

Bioinformatics

• Uniprot:
  • Q9NYK1()