Recombinant Human TLR6 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TLR6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When peptidoglycan is immobilized at 10 µg/mL (100 µL/well), the concentration of Recombinant Human TLR6 Fc Chimera that produces 50% of the optimal binding response is approximately 0.05‑0.25 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human TLR6 protein
  

  Human TLR6 (Asn32-Asn583) Accession # AAY88759	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: AAY88759
• N-terminal Sequence: Asn32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: TLR6
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 89.9 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TLR6 Fc Chimera Protein, CF

• CD286 antigen
• CD286
• TLR6
• toll-like receptor 6

Background

The Toll‑like family of molecules serve as pattern recognition receptors that activate the immune system in response to microbial pathogens. Toll‑like receptors (TLRs) are type I transmembrane proteins in either the plasma membrane or the membranes of endosomal structures. TLRs contain a large number of leucine‑rich repeats (LRRs) in their extracellular domains (ECD) and a cytoplasmic tail with one signal transducing Toll/IL‑1 receptor (TIR) domain (1, 2). Mature human TLR6 is an approximately 115 kDa protein that consists of a 555 amino acid (aa) ECD, a 21 aa transmembrane segment, and a 189 aa cytoplasmic domain (3). Within the ECD, human TLR6 shares 72% aa sequence identity with mouse and rat TLR6. TLR6 is expressed on the surface of macrophages, monocytes, neutrophils, dermal endothelial cells, and alveolar epithelial cells in ligand‑independent association with TLR2 (4‑7). TLR2 also associates with TLR1, creating a functional receptor complex with specificity for related microbial ligands (4, 8, 9). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycans, and glycosylphosphatidylinositols (4‑6, 10‑13). TLR6 plays an important role in the control of Th17 responses and IL‑23 production during allergic inflammation and fungal‑induced asthma (7).

1. Mills, K.H. (2011) Nat. Rev. Immunol. 11:807.
2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
3. Takeuchi, O. et al. (1999) Gene 231:59.
4. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
5. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
6. Bulut, Y. et al. (2001) J. Immunol. 167:987.
7. Moreira, A.P. et al. (2011) J. Clin. Invest. 121:4420.
8. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
9. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
10. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
11. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
13. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.

Publications for TLR6 (7755-TR)(1)

Publication using 7755-TR

• C Mayslich, PA Grange, M Castela, AG Marcelin, V Calvez, N Dupin Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation International Journal of Molecular Sciences, 2022-05-03;23(9):. 2022-05-03 [PMID: 35563458] (Bioassay, Western Blot (Detection), Human)

Bioinformatics

• Gene Symbol: TLR6
• Uniprot:
  • AAY88759()