Recombinant Human TLR10 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TLR10 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human TLR10 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human TLR1 Fc Chimera (Catalog # 1484-TR) that produces 50% of the optimal binding response is approximately 0.5‑3 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human TLR10 protein
  

  Human TLR10 (Asp20-Thr576) Accession # Q9BXR5	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9BXR5
• N-terminal Sequence: Asp20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: TLR10
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 91.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 115-135 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TLR10 Fc Chimera Protein, CF

• CD290 antigen
• CD290
• MGC104967
• MGC126398
• MGC126399
• TLR10
• toll-like receptor 10

Background

TLR10 (Toll‑like receptor 10, designated CD290) is a 90‑100 kDa type I transmembrane glycoprotein that is a member of the TLR1 subfamily within the TLR family of pathogen-associated molecular pattern (PAMP) receptors (1‑3). Human TLR10 is synthesized as a precursor with a 19 amino acid (aa) signal sequence, an extracellular domain (ECD, aa 20‑576) with 15 or more leucine‑rich repeats (LRR), an LRRCT (LRR C‑terminal) domain and nine potential sites for N‑linked glycosylation, a transmembrane domain, and a cytoplasmic region (aa 598‑811) with a TIR (Toll/IL‑1 receptor) domain (1, 3). Human TLR10 shares 71%, 81%, 82% and 83% aa sequence identity with rat, bovine, porcine and canine TLR10, respectively. The mouse TLR10 gene is nonfunctional (2). TLR10 is expressed on B cells and cell lines, except for early pre‑B cells, and is more highly expressed on activated than resting cells (2, 4). It is also expressed on FOXP3⁺ regulatory T cells, placental trophoblasts, and CD1a⁺ and plasmacytoid dendritic cells (DC), but not on monocyte‑derived DC (2, 5, 6). Among PAMPs, TLR10 is thought to recognize triacylated bacterial lipoproteins (as demonstrated by recognition of the synthetic molecule Pam₃CSK₄), gram positive bacterial peptidoglycans, and other microbially-derived agonists shared with TLR1 (3, 6, 7). It is found as a homodimer or heterodimer with TLR1 or TLR2, associating through a region within the extracellular LRR repeats (2, 3, 7). It interacts directly with MYD88, leading to production of inflammatory cytokines (2, 7).

1. Chuang, T. and R.J. Ulevitch (2001) Biochim. Biophys. Acta 1518:157.
2. Hasan, U. et al. (2005) J. Immunol. 174:2942.
3. Govindaraj, R.G. et al. (2010) PLoS ONE 5:e12713.
4. Bourke, E. et al. (2003) Blood 102:956.
5. Bell, M.P. et al. (2007) J. Immunol. 79:1893.
6. Mulla, M.J. et al. (2013) Am. J. Reprod. Immunol. 69:449.
7. Guan, Y. et al. (2010) J. Immunol. 184:5094.

Bioinformatics

• Gene Symbol: TLR10
• Uniprot:
  • Q9BXR5()