Recombinant Human TGF-beta 3 Protein, CF

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Recombinant Human TGF-beta 3 (Catalog # 243-B3/CF) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.01-0.04 ng/mL.
1 μg/lane of Recombinant Human TGF-beta 3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TGF-beta 3 Protein, CF Summary

Additional Information
A New and Improved rh TGF-beta 3 is Now Available! It is CHO-expressed and competitively priced.
Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.01-0.04 ng/mL.
The specific activity of recombinant human TGF-beta 3 is approximately 2.2 x 104 IU/μg, which is calibrated against recombinant human TGF-beta 3 WHO International Standard (NIBSC code: 09/234).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human TGF-beta 3 protein
Ala301-Ser412 (Tyr340Phe)
Accession #
N-terminal Sequence
Ala301
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions
Publications
Read Publications using
243-B3/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute 2 µg vials at 20 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. Reconstitute 10 µg or larger vials at 50 µg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 3 Protein, CF

  • ARVD
  • FLJ16571
  • TGFB3
  • TGFbeta 3
  • TGF-beta 3
  • TGF-beta3
  • TGF-beta-3
  • transforming growth factor beta-3
  • transforming growth factor, beta 3

Background

TGF­ beta 3 (transforming growth factor-beta 3) is a member of a TGF­-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and ­-beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial­-mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-­ beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF-­ beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin­like convertase to generate a 220 aa latency­associated peptide (LAP) and a 112 aa mature TGF­-beta 3 (10, 11). Mature human TGF­-beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF-­ beta 3, respectively. TGF-beta 3 is secreted as a complex with LAP. This latent form of TGF-beta 3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin-1, and a subset of integrins (12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-­ beta RI or ALK-­1, to modulate transcription through Smad phosphorylation (13-15). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-­ beta RIII and endoglin) and the subsequent initiation of Smad-­dependent or -independent signaling pathways (14, 16, 17).

  1. Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
  2. Doetschman, T. et al. (2012) Genesis 50:59.
  3. Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
  4. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  5. Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
  6. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  7. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  8. Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
  9. Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
  10. Derynck, R. et al. (1988) EMBO J. 7:3737.
  11. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  12. Oklu, R. and R. Hesketh (2000) Biochem. J. 352 Pt 3:601.
  13. Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
  14. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  15. Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
  16. Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
  17. Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.

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Publications for TGF-beta 3 (243-B3/CF)(71)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Bovine, Canine, Equine, N/A, Rabbit, Sheep, Xenopus.

We have publications tested in 7 applications: Bioassay, Cell Culture Substrate, ELISA Standard, In Vivo, Surface Plasmon Resonance, WB Ctrl, differentiation.


Filter By Application
Bioassay
(61)
Cell Culture Substrate
(1)
ELISA Standard
(2)
In Vivo
(4)
Surface Plasmon Resonance
(1)
WB Ctrl
(1)
differentiation
(1)
All Applications
Filter By Species
Human
(39)
Mouse
(12)
Rat
(6)
Bovine
(1)
Canine
(3)
Equine
(3)
N/A
(4)
Rabbit
(1)
Sheep
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 71. Show All 71 Publications.
Publications using 243-B3/CF Applications Species
J Riegger, HG Palm, RE Brenner The functional role of chondrogenic stem/progenitor cells: novel evidence for immunomodulatory properties and regenerative potential after cartilage injury Eur Cell Mater, 2018;36(0):110-127. 2018 [PMID: 30215457] (Bioassay, Human) Bioassay Human
H Lin, E Hay, A Latourte, T Funck-Bren, W Bouaziz, HK Ea, AM Khatib, P Richette, M Cohen-Sola Proprotein convertase furin inhibits matrix metalloproteinase 13 in a TGF?-dependent manner and limits osteoarthritis in mice Sci Rep, 2018;8(1):10488. 2018 [PMID: 29992982] (Bioassay, Mouse) Bioassay Mouse
G Collo, L Cavalleri, M Zoli, U Maskos, E Ratti, E Merlo Pich Alpha6-Containing Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Structural Plasticity in Mouse and Human iPSC-Derived Dopaminergic Neurons Front Pharmacol, 2018;9(0):572. 2018 [PMID: 29910731] (Bioassay, Human) Bioassay Human
M Desancé, R Contentin, L Bertoni, T Gomez-Ledu, T Branly, S Jacquet, JM Betsch, A Batho, F Legendre, F Audigié, P Galéra, M Demoor Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy Int J Mol Sci, 2018;19(2):. 2018 [PMID: 29439436] (Bioassay, Equine) Bioassay Equine
J Nishida, K Miyazono, S Ehata Decreased TGFBR3/betaglycan expression enhances the metastatic abilities of renal cell carcinoma cells through TGF-?-dependent and -independent mechanisms Oncogene, 2018;0(0):. 2018 [PMID: 29391598] (Bioassay, Human) Bioassay Human
T Branly, R Contentin, M Desancé, T Jacquel, L Bertoni, S Jacquet, F Mallein-Ge, JM Denoix, F Audigié, M Demoor, P Galéra Improvement of the Chondrocyte-Specific Phenotype upon Equine Bone Marrow Mesenchymal Stem Cell Differentiation: Influence of Culture Time, Transforming Growth Factors and Type I Collagen siRNAs on the Differentiation Index Int J Mol Sci, 2018;19(2):. 2018 [PMID: 29389887] (Bioassay, Equine) Bioassay Equine
U Mayer, A Benditz, S Grässel miR-29b regulates expression of collagens I and III in chondrogenically differentiating BMSC in an osteoarthritic environment Sci Rep, 2017;7(1):13297. 2017 [PMID: 29038440] (Bioassay, Human) Bioassay Human
D Parate, A Franco-Obr, J Fröhlich, C Beyer, AA Abbas, T Kamarul, JHP Hui, Z Yang Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields Sci Rep, 2017;7(1):9421. 2017 [PMID: 28842627] (Bioassay, Human) Bioassay Human
K Hino, K Horigome, M Nishio, S Komura, S Nagata, C Zhao, Y Jin, K Kawakami, Y Yamada, A Ohta, J Toguchida, M Ikeya Activin-A enhances mTOR signaling to promote aberrant chondrogenesis in fibrodysplasia ossificans progressiva J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28758906] (Bioassay, Human) Bioassay Human
S Stich, A Loch, SJ Park, T Häupl, J Ringe, M Sittinger Characterization of single cell derived cultures of periosteal progenitor cells to ensure the cell quality for clinical application PLoS ONE, 2017;12(5):e0178560. 2017 [PMID: 28562645] (Bioassay, Human) Bioassay Human
Show All 71 Publications.

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Bioinformatics

Gene Symbol TGFB3
Uniprot