Recombinant Human PBEF/Visfatin Protein, CF

Recombinant Human PBEF/Visfatin (Catalog # 8424-VF) induces VEGF secretion by PC‑3 human prostate cancer cells. The ED50 for this effect is 1-5 μg/mL

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PBEF/Visfatin Protein, CF Summary

• Details of Functionality: Measured by its ability to induce VEGF secretion by PC‑3 human prostate cancer cells. The ED₅₀ for this effect is 1-5 μg/mL
• Source: E. coli-derived human PBEF/Visfatin protein
  Asn2-His491, with an N-terminal Met and 6-His tag
• Accession #: P43490
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: NAMPT
• Purity: >90%, by SDS-PAGE with silver staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA, TCEP and Tween.
• Purity: >90%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PBEF/Visfatin Protein, CF

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

PBEF, also called Visfatin and NAMPT, is a dimeric type II phosphoribosyltransferase (1). It was initially identified as a secreted cytokine that synergized with IL-7 and SCF to stimulate early stage B cells (2). Human PBEF is predominantly expressed in bone marrow, liver, and muscle, with lower levels found in placenta, kidney, heart, and lung (2). Human PBEF shares 96% and 95% amino acid sequence identity with mouse and rat PBEF, respectively. PBEF has been identified both as an intracellular and as an extracellular protein. Within the cell, PBEF converts nicotinamide to nicotinamide mononucleotide (NMN), which is the rate limiting step in the production of nicotinamide adenine dinucleotide (NAD⁺) (3, 4). In the extracellular environment, PBEF has been reported to increase the production of inflammatory cytokines and have an important role in the development of T and B cells (5-7). Additionally, PBEF may promote angiogenesis in vivo in an ERK1/2-dependent manner (8). PBEF has been implicated in several inflammatory diseases, including rheumatoid arthritis, osteoarthritis, inflammatory bowel disease, and sepsis (9). PBEF over‑expression has been observed in many cancer types (10).

1. Wang, T. et al. (2006) Nat. Struct. Mol. Biol. 13:661.
2. Samal, B. et al. (1994) Mol. Cell. Biol. 14:1431.
3. Rongvaux, A. et al. (2002) Eur. J. Immunol. 32:3225.
4. Revollo, J.R. et al. (2007) Cell Metab. 6:363.
5. Ognjanovic, S. and G.D. Bryant-Greenwood (2002) Am. J. Obstet. Gynecol. 187:1051.
6. Tanaka, M. et al. (2007) Biochem. Biophys. Res. Commun. 359:194.
7. Rongvaux, A. et al. (2008) J. Immunol. 181:4685.
8. Kim, S.R. et al. (2007) Biochem. Biophys. Res. Commun. 357:150.
9. Sun, Z. et al. (2013) Cytokine Growth Factor Rev. 24:433.
10. Shackelford, R.E. et al. (2013) Genes Cancer 4:447.

Publications for PBEF/Visfatin/NAMPT (8424-VF)(3)

Publications using 8424-VF

• Mlyczy?ska, E;Rytelewska, E;Zaobidna, E;Respekta-D?ugosz, N;Kopij, G;Dobrzy?, K;Kie?un, M;Smoli?ska, N;Kami?ski, T;Rak, A; In vitro effect of visfatin on endocrine functions of the porcine corpus luteum Scientific reports 2024-06-26 [PMID: 38926439] (Bioassay, Porcine)
• MJ Butler, H Volkoff The role of visfatin/ NAMPT in the regulation of feeding in goldfish (Carassius auratus) Peptides, 2022-11-26;160(0):170919. 2022-11-26 [PMID: 36503895] (In Vivo, Goldfish)
• C Shi, H Wu, D Du, HJ Im, Y Zhang, B Hu, H Chen, X Wang, Y Liu, P Cao, Y Tian, X Shen, R Gao, AJ van Wijnen, X Ye, W Yuan Nicotinamide Phosphoribosyltransferase Inhibitor APO866 Prevents IL-1?-Induced Human Nucleus Pulposus Cell Degeneration via Autophagy Cell. Physiol. Biochem., 2018-09-27;49(6):2463-2482. 2018-09-27 [PMID: 30261504] (Bioassay, Human)

Bioinformatics

• Gene Symbol: NAMPT
• Uniprot:
  • P43490()