Recombinant Human Noggin Fc Chimera Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Noggin Fc Chimera Protein, CF Summary

Additional Information
A New and improved rhNoggin is Now Available! The new protein has ~2 fold better activity, higher purity, and no tag!
New Product 6057-NG
Details of Functionality
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.1-0.4 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).
Source
Mouse myeloma cell line, NS0-derived human Noggin protein
Human Noggin
(Gln28-Cys232)
Accession # Q13253
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NOG
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
49.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
58-61 kDa, reducing conditions
Publications
Read Publications using
3344-NG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Noggin Fc Chimera Protein, CF

  • NOG
  • Noggin
  • SYM1
  • symphalangism 1 (proximal)
  • synostoses (multiple) syndrome 1
  • SYNS1

Background

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin‑binding segment and a C-terminal cysteine-knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity, antagonizing BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) without feeder layers or conditioned medium, but with addition of FGF basic, addition of Noggin to antagonize BMP activity allows hESC to maintain their undifferentiated, pluripotent state (9, 10).

  1. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  2. Groppe, J. et al. (2002) Nature 420:636.
  3. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
  4. Brunet, L. J. et al. (1998) Science 280:1455.
  5. McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
  6. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
  7. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
  8. Gong, Y. et al. (1999) Nat. Genet. 21:302.
  9. Xu, R.-H. et al. (2005) Nat. Methods 2:185.
  10. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.

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Publications for Noggin (3344-NG)(13)

We have publications tested in 5 confirmed species: Human, Bovine, Chicken, Porcine, Xenopus.

We have publications tested in 4 applications: Bioassay, In Ovo, In Vivo, cell culture.


Filter By Application
Bioassay
(9)
In Ovo
(1)
In Vivo
(2)
cell culture
(1)
All Applications
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Human
(7)
Bovine
(1)
Chicken
(2)
Porcine
(1)
Xenopus
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 3344-NG Applications Species
P Scerbo, L Marchal, L Kodjabachi Lineage commitment of embryonic cells involves MEK1-dependent clearance of pluripotency regulator Ventx2 Elife, 2017;6(0):. 2017 [PMID: 28654420] (In Vivo, Xenopus) In Vivo Xenopus
Guezguez A, Pare F, Benoit Y, Basora N, Beaulieu J Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res, 2014;322(2):355-64. 2014 [PMID: 24534551] (Bioassay, Human) Bioassay Human
Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 2012;21(6):1321-39. 2012 [PMID: 22195604] (cell culture, Human) cell culture Human
Kelly OG, Chan MY, Martinson LA Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat. Biotechnol., 2011;29(8):750-6. 2011 [PMID: 21804561] (Bioassay, Human) Bioassay Human
Kelly MA, Hirschi KK Signaling hierarchy regulating human endothelial cell development. Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24. 2009 [PMID: 19213939] (Bioassay, Human) Bioassay Human
Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 2009;583(3):561-7. 2009 [PMID: 19162023] (Bioassay, Human) Bioassay Human
Yao Y, Shao ES, Jumabay M, Shahbazian A, Ji S, Bostrom KI High-density lipoproteins affect endothelial BMP-signaling by modulating expression of the activin-like kinase receptor 1 and 2. Arterioscler. Thromb. Vasc. Biol., 2008;28(12):2266-74. 2008 [PMID: 18948634] (Bioassay, Bovine) Bioassay Bovine
Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev., 2008;22(2):152-65. 2008 [PMID: 18198334] (Bioassay, Human) Bioassay Human
Wen Z, Han L, Bamburg JR, Shim S, Ming GL, Zheng JQ BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin. J. Cell Biol., 2007;178(1):107-19. 2007 [PMID: 17606869] (Bioassay, Xenopus) Bioassay Xenopus
Knobloch J, Shaughnessy JD, Ruther U Thalidomide induces limb deformities by perturbing the Bmp/Dkk1/Wnt signaling pathway. FASEB J., 2007;21(7):1410-21. 2007 [PMID: 17283219] (In Vivo, Chicken) In Vivo Chicken
Show All 13 Publications.

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Bioinformatics

Gene Symbol NOG
Entrez
Uniprot