Recombinant Human MIF Protein


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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human MIF Protein Summary

Details of Functionality
Bioassay data are not available.
E. coli-derived human MIF protein
Pro2-Ala115, with an N-terminal Met
Accession #
N-terminal Sequence
Met1 & Pro2
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
12.4 kDa & 12.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
289-MF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MES and NaCl with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MIF Protein

  • EC
  • EC
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MIF
  • MMIF
  • Phenylpyruvate tautomerase


MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4 - 7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49 - 65. On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine and rat MIF, respectively.

  1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
  2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
  3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
  4. Kozak, C.A. et al. (1995) Genomics 27:405.
  5. Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
  6. Paralkar, V. and G. Wistow (1994) Genomics 19:48.
  7. Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
  8. Flieger, O. et al. (2003) FEBS Lett. 551:78.
  9. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
  10. Sun, H-W. et al. (1996) Protein Eng. 9:631.
  11. Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
  12. Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
  13. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
  14. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
  15. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  16. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.

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Publications for MIF (289-MF)(28)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, N/A.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), In Vivo, Surface Plasmon Resonance (SPR, Western Blot.

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ELISA (Standard)
In Vivo
Surface Plasmon Resonance (SPR
Western Blot
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Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 289-MF Applications Species
HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth Cells, 2020;9(6):. 2020 [PMID: 32481584] (Bioassay, Human) Bioassay Human
H Chen, W Xia, M Hou LncRNA-NEAT1 from the competing endogenous RNA network promotes cardioprotective efficacy of mesenchymal stem cell-derived exosomes induced by macrophage migration inhibitory factor via the miR-142-3p/FOXO1 signaling pathway Stem Cell Res Ther, 2020;11(1):31. 2020 [PMID: 31964409] (Bioassay, Human) Bioassay Human
LA Hernández-, S García-Are, R Bucala, MA Llamas-Cov, U De la Cruz, E Oregon-Rom, S Cerpa-Cruz, I Parra-Roja, A Plascencia, JF Muñoz-Vall Functional MIF promoter haplotypes modulate Th17-related cytokine expression in peripheral blood mononuclear cells from control subjects and rheumatoid arthritis patients Cytokine, 2019;115(0):89-96. 2019 [PMID: 30467094] (Bioassay, Human) Bioassay Human
L Rijvers, MJ Melief, RM van der Vu, M Stéphant, J van Langel, AF Wierenga-W, JM Hogervorst, AJ Geurts-Moe, FCGJ Sweep, RQ Hintzen, MM van Luijn The macrophage migration inhibitory factor pathway in human B cells is tightly controlled and dysregulated in multiple sclerosis Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30160778] (Human) Human
F Ietta, EAV Ferro, E Bevilacqua, L Benincasa, E Maioli, L Paulesu Role of the Macrophage Migration Inhibitory Factor (MIF) in the survival of first trimester human placenta under induced stress conditions Sci Rep, 2018;8(1):12150. 2018 [PMID: 30108299] (Bioassay, Human) Bioassay Human
J Lang, Y Cheng, A Rolfe, C Hammack, D Vera, K Kyle, J Wang, TB Meissner, Y Ren, C Cowan, H Tang An hPSC-Derived Tissue-Resident Macrophage Model Reveals Differential Responses of Macrophages to ZIKV and DENV Infection Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29983385] (Bioassay, Human) Bioassay Human
JA Fraser, S Kemp, L Young, M Ross, M Prach, GR Hutchison, E Malone Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations Sci Rep, 2018;8(1):7506. 2018 [PMID: 29760395] (ELISA (Standard)) ELISA (Standard)
SU Noh, WY Lee, WS Kim, YT Lee, KJ Yoon Expression of Macrophage Migration Inhibitory Factor in Footpad Skin Lesions with Diabetic Neuropathy Mol Pain, 2018;0(0):1744806918775. 2018 [PMID: 29690804] (Bioassay, Human) Bioassay Human
S Sugita, K Makabe, Y Iwasaki, S Fujii, M Takahashi Natural Killer Cell Inhibition by HLA-E Molecules on Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells Invest. Ophthalmol. Vis. Sci., 2018;59(5):1719-1731. 2018 [PMID: 29610856] (Bioassay, Human) Bioassay Human
A Sparkes, P De Baetsel, L Brys, I Cabrito, YG Sterckx, S Schoonoogh, S Muylderman, G Raes, R Bucala, P Vanlandsch, JA Van Ginder, B Stijlemans Novel half-life extended anti-MIF nanobodies protect against endotoxic shock FASEB J., 2018;0(0):fj201701189R. 2018 [PMID: 29401625] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
Show All 28 Publications.

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Gene Symbol MIF