CD74 Antibody (PIN.1)

Images

 
Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Ramos whole cell protein was separated on a 4-12% gel by SDS-PAGE and protein transferred to PVDF. The membrane was probed with anti-CD74 at 2 ug/ml and detected with ...read more
Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml ...read more
Flow Cytometry: CD74 Antibody (PIN.1) [NB100-1985] - CD74 in human PBMCs (gated on all monocytes). Orange represents isotype control and blue represents anti-CD74 antibody staining.
Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).
Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ...read more
Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using mouse monoclonal CD74 antibody (clone ...read more

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
PIN.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD74 Antibody (PIN.1) Summary

Immunogen
Peptide corresponding to residues 12-27 of CD74, human invariant chain (short form).
Epitope
The epitope is in a region of the cytoplasmic tail of human CD74 which is common to all isoforms.
Localization
Type II membrane protein
Specificity
This detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD74
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point. In WB this detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.
Publications
Read Publications using
NB100-1985 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human. Does not react with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD74 Antibody (PIN.1)

  • CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
  • CD74 antigen
  • CD74 molecule, major histocompatibility complex, class II invariant chain
  • CD74
  • CLIP
  • DHLAG
  • DHLAGgamma chain of class II antigens
  • HLA class II histocompatibility antigen gamma chain
  • HLADG
  • HLA-DR antigens-associated invariant chain
  • HLA-DR-gamma
  • Ia antigen-associated invariant chain
  • Ia-associated invariant chain
  • Ia-gamma
  • Ii
  • INVG34
  • MHC HLA-DR gamma chain
  • p33

Background

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41kDa, depending on genetic splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD74 Antibody (NB100-1985)(4)

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Product General Protocols

View specific protocols for CD74 Antibody (NB100-1985): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD74 Antibody (NB100-1985) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1985AF405
Alexa Fluor 488 Labeled NB100-1985AF488
Alexa Fluor 647 Labeled NB100-1985AF647
Alexa Fluor 700 Labeled NB100-1985AF700
Allophycocyanin Labeled NB100-1985APC
Biotin Labeled NB100-1985B
DyLight 350 Labeled NB100-1985UV
DyLight 405 Labeled NB100-1985V
DyLight 488 Labeled NB100-1985G
DyLight 550 Labeled NB100-1985R
DyLight 650 Labeled NB100-1985C
DyLight 680 Labeled NB100-1985FR
DyLight 755 Labeled NB100-1985IR
HRP Labeled NB100-1985H
PE Labeled NB100-1985PE
PerCP Labeled NB100-1985PCP

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Bioinformatics Tool for CD74 Antibody (NB100-1985)

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Research Areas for CD74 Antibody (NB100-1985)

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Blogs on CD74.

CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.

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Bioinformatics

Gene Symbol CD74
Entrez
Uniprot