MIF Antibody (12302) [Unconjugated]

Western blot shows lysate of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Exosome Standards (HEK293) (NBP3-11684), Exosome Standards (LNCaP) (NBP3-11687) and K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for MIF at approximately 11 kDa (as indicated) using 10 µg/ml of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MIF at approximately 12 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 12302
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MIF Antibody (12302) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MIF
  Pro2-Ala115
  Accession # P14174
• Specificity: Detects human MIF in Western blots. In Western blots, this antibody shows approximately 25% cross-reactivity with recombinant mouse MIF.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MIF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIF Antibody (12302) [Unconjugated]

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MIF
• MMIF
• Phenylpyruvate tautomerase

Background

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.

1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
4. Kozak, C.A. et al. (1995) Genomics 27:405.
5. Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
6. Paralkar, V. and G. Wistow (1994) Genomics 19:48.
7. Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
8. Flieger, O. et al. (2003) FEBS Lett. 551:78.
9. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
10. Sun, H-W. et al. (1996) Protein Eng. 9:631.
11. Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
12. Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
13. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
14. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
15. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
16. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIF Antibody (MAB289)(28)

Publications using MAB289

• Blandin, A;Amosse, J;Froger, J;Hilairet, G;Durcin, M;Fizanne, L;Ghesquière, V;Prieur, X;Chaigneau, J;Vergori, L;Dray, C;Pradère, JP;Blandin, S;Dupont, J;Ducluzeau, PH;Dubois, S;Boursier, J;Cariou, B;Le Lay, S; Extracellular vesicles are carriers of adiponectin with insulin-sensitizing and anti-inflammatory properties Cell reports 2023-08-29 [PMID: 37605533] (Western Blot, Transgenic Mouse)
• Lu Wang, Shuangshuang Li, Huanhua Luo, Qi Lu, Shuwen Yu PCSK9 promotes the progression and metastasis of colon cancer cells through regulation of EMT and PI3K/AKT signaling in tumor cells and phenotypic polarization of macrophages Journal of Experimental & Clinical Cancer Research 10/14/2022 [PMID: 36242053]
• Ashita Nair, Jiyoon Bu, Piper A. Rawding, Steven C. Do, Hangpeng Li, Seungpyo Hong Cytochalasin B Treatment and Osmotic Pressure Enhance the Production of Extracellular Vesicles (EVs) with Improved Drug Loading Capacity Nanomaterials (Basel) 12/21/2021 [PMID: 35009953]
• GF Jang, JS Crabb, B Hu, B Willard, H Kalirai, AD Singh, SE Coupland, JW Crabb Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers Cancers, 2021-07-14;13(14):. 2021-07-14 [PMID: 34298739] (Western Blot, Human)
• HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth Cells, 2020-05-28;9(6):. 2020-05-28 [PMID: 32481584] (Neutralization, Human)
• A. Vilotic, Z. Bojic-Trbojevic, L. Vicovac, M. Jovanovic Jovanovic Krivokuca Macrophage migration inhibitory factor is differentially expressed in normal and choriocarcinoma trophoblast cells Neoplasma 1/1/2020 [PMID: 31973534]
• W Ha, H Sevim-Nalk, AM Zaman, K Matsuda, M Khasraw, AK Nowak, L Chung, RC Baxter, KL McDonald Ibudilast sensitizes glioblastoma to temozolomide by targeting Macrophage Migration Inhibitory Factor (MIF) Sci Rep, 2019-02-27;9(1):2905. 2019-02-27 [PMID: 30814573] (IHC-P, Human)
• Qiao C, Li S, Lu H et al. Laminar Flow Attenuates Macrophage Migration Inhibitory Factor Expression in Endothelial Cells. Sci Rep 2/5/2018 [PMID: 29403061]
• JS Kim, J Choi, HJ Hahn, YB Lee, DS Yu, JW Kim Association of Macrophage Migration Inhibitory Factor Polymorphisms with Total Plasma IgE Levels in Patients with Atopic Dermatitis in Korea PLoS ONE, 2016-09-06;11(9):e0162477. 2016-09-06 [PMID: 27598332] (ELISA Development (Capture), Human)
• Thomas Breuer, Christoph Emontzpohl, Mark Coburn, Carina Benstoem, Rolf Rossaint, Gernot Marx et al. Xenon triggers pro-inflammatory effects and suppresses the anti-inflammatory response compared to sevoflurane in patients undergoing cardiac surgery Critical Care 12/1/2015 [PMID: 26467531]
• Kim B, Rongisch R, Hager S, Grieb G, Nourbakhsh M, Rennekampff H, Bucala R, Bernhagen J, Pallua N Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation. PLoS ONE, 2015-09-08;10(9):e0137366. 2015-09-08 [PMID: 26348853] (ELISA Development (Capture), IHC-P, Human)
• Crabb J, Hu B, Crabb J, Triozzi P, Saunthararajah Y, Tubbs R, Singh A iTRAQ Quantitative Proteomic Comparison of Metastatic and Non-Metastatic Uveal Melanoma Tumors. PLoS ONE, 2015-08-25;10(8):e0135543. 2015-08-25 [PMID: 26305875] (Western Blot, Human)
• Christian Stoppe, Steffen Rex, Andreas Goetzenich, Sandra Kraemer, Christoph Emontzpohl, Josefin Soppert et al. Interaction of MIF Family Proteins in Myocardial Ischemia/Reperfusion Damage and Their Influence on Clinical Outcome of Cardiac Surgery Patients Antioxidants & Redox Signaling 10/10/2015 [PMID: 26234719]
• Oliveira , Camila S, de Bock , Charles, Molloy , Timothy, Sadeqzadeh , Elham, Geng , Xin Yan, Hersey , Peter, Zhang , Xu Dong, Thorne , Rick F Macrophage migration inhibitory factor engages PI3K/Akt signalling and is a prognostic factor in metastatic melanoma. BMC Cancer, 2014-08-29;14(0):630. 2014-08-29 [PMID: 25168062] (Western Blot, Human)
• Dumitru CA, Bankfalvi A, Gu X et al. AHNAK and Inflammatory Markers Predict Poor Survival in Laryngeal Carcinoma. PLoS One 1/1/2013 [PMID: 23409183]
• Christian Stoppe, Thilo Werker, Rolf Rossaint, Florian Dollo, Hongqi Lue, Willibald Wonisch et al. What Is the Significance of Perioperative Release of Macrophage Migration Inhibitory Factor in Cardiac Surgery? Antioxidants & Redox Signaling 7/20/2013 [PMID: 23157710]
• Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (Luminex Development, Human)
• Rachel Damico, Tiffany Simms, Bo S. Kim, Zenar Tekeste, Henry Amankwan, Mahendra Damarla et al. p53 mediates cigarette smoke-induced apoptosis of pulmonary endothelial cells: inhibitory effects of macrophage migration inhibitor factor American Journal of Respiratory Cell and Molecular Biology 3/1/2011 [PMID: 20448056]
• David Simons, Gerrit Grieb, Mihail Hristov, Norbert Pallua, Christian Weber, Jürgen Bernhagen et al. Hypoxia-induced endothelial secretion of macrophage migration inhibitory factor and role in endothelial progenitor cell recruitment Journal of Cellular and Molecular Medicine 3/1/2011 [PMID: 20178462]
• Ren Y, Law S, Huang X, Lee PY, Bacher M, Srivastava G, Wong J Macrophage migration inhibitory factor stimulates angiogenic factor expression and correlates with differentiation and lymph node status in patients with esophageal squamous cell carcinoma. Ann. Surg., 2005-07-01;242(1):55-63. 2005-07-01 [PMID: 15973102] (ICC, Human)
• Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis. Histopathology, 2005-06-01;46(6):659-67. 2005-06-01 [PMID: 15910597] (Neutralization, Human)
• Wadgaonkar R, Dudek SM, Zaiman AL, Linz-McGillem L, Verin AD, Nurmukhambetova S, Romer LH, Garcia JG Intracellular interaction of myosin light chain kinase with macrophage migration inhibition factor (MIF) in endothelium. J. Cell. Biochem., 2005-07-01;95(4):849-58. 2005-07-01 [PMID: 15838879] (Immunoprecipitation, Bovine, Human)
• Matsumoto K, Maruyama N, Maruyama T, Ohnishi Y, Nonaka S, Inoshita A, Ito K, Kitajima S, Abe M, Satomura A, Fujita T Elevated macrophage migration inhibitory factor (MIF) levels in the urine of patients with focal glomerular sclerosis. Clin. Exp. Immunol., 2005-02-01;139(2):338-47. 2005-02-01 [PMID: 15654833] (ELISA Development, IHC, Human)
• Riedemann NC, Guo RF, Gao H, Sun L, Hoesel M, Hollmann TJ, Wetsel RA, Zetoune FS, Ward PA Regulatory role of C5a on macrophage migration inhibitory factor release from neutrophils. J. Immunol., 2004-07-15;173(2):1355-9. 2004-07-15 [PMID: 15240730] (ICC, Human)
• Chuang CC, Hung CJ, Tsai MC, Chuang YC High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor? Crit. Care Med., 2004-03-01;32(3):734-9. 2004-03-01 [PMID: 15090955] (ELISA Development, Human)
• Ashcroft GS, Mills SJ, Lei K, Gibbons L, Jeong MJ, Taniguchi M, Burow M, Horan MA, Wahl SM, Nakayama T Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor. J. Clin. Invest., 2003-05-01;111(9):1309-18. 2003-05-01 [PMID: 12727922] (ELISA Development, Mouse)
• Matsumoto K, 2019, Kanmatsuse K Urinary levels of macrophage migration inhibitory factor in patients with IgA nephropathy. 7587, 2002-10-01;92(2):309-15. 2002-10-01 [PMID: 12218308] (ELISA Development, Human)
• Wang L, Das H, Kamath A, Li L, Bukowski JF Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production. J. Immunol., 2002-05-15;168(10):4889-96. 2002-05-15 [PMID: 11994438] (Human)

Review for MIF Antibody (MAB289) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/07/2021

Summary

• Application: Western Blot
• Sample Tested: THP-1 human acute monocytic leukemia cell line
• Species: Human
• Lot: 12302

Bioinformatics

• Gene Symbol: MIF
• Uniprot:
  • P14174()