| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB289-SP | |||
| Carrier Free | MAB289-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | SMF00B | |||
| Carrier Free | PDMF00B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 289-MF-01M/CF | |||
| Carrier Free | 289-MF-002/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-289-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1978-MF-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81832-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2892-SP | |||
![N/A MIF [HRP]](http://images.novusbio.com/fullsize2/DATA_MIF_DMF00_ELISA_763.jpg "N/A MIF [HRP]")
![N/A MIF [HRP]](http://images.novusbio.com/fullsize2/MIF_DMF00_ELISA_391.jpg)
![N/A MIF [Biotin]](http://images.novusbio.com/fullsize2/DATA_MIF_DY1978_ELISA_1744.jpg "N/A MIF [Biotin]")
![Western Blot: MIF Antibody [NBP1-81832] - Analysis in human cell lines HEK293 and U-251MG using Anti-MIF antibody. Corresponding MIF RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-81832-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: MIF Antibody [NBP1-81832] - Analysis in human cell lines HEK293 and U-251MG using Anti-MIF antibody. Corresponding MIF RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MIF Antibody [NBP1-81832] - Expression and purification of MaMIF. Western blot analysis with isotype-matched primary antibody shows the background level of antibody binding to the protein. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-51947-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-81832-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: MIF Antibody [NBP1-81832] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81832-img0015.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-81832-img0019.jpg)
![Western Blot: MIF Antibody [NBP1-81832] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-81832-img0011.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-81832-img0016.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human kidney moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-81832-img0017.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-81832-img0018.jpg)
![Western Blot: MIF Antibody [NBP1-81832] - Expression & purification of MaMIF. (A) SDS-PAGE analysis of purified MaMIF suggesting an approximate molecular mass of ~12.5 kDa for the monomer. (B) Superdex 75 10/300 GL analytical size-exclusion chromatography profile of MaMIF suggesting an apparent molecular mass of ~39 kDa in solution, corresponding to the size of a trimeric form. (C) CD spectrum of MaMIF confirming a properly folded protein. The pie chart in the inset shows the secondary structure make-up of the protein. (D) Western blot analysis with polyclonal anti-His & anti-MIF antibodies confirming the expressed protein to be MaMIF. (E) Western blot analysis with isotype-matched primary antibody shows the background level of antibody binding to the protein. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31664114), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81832_rabbit-polyclonal-mif-antibody-31020241534334.jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0005.jpg "Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry: MIF Antibody [NB100-1789] - Co-localization of CXCR4 and MIF in urothelium. Sections from rats treated with saline or CYP are shown. MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) and an overlay panel combining both immunostaining and a DAPI nuclear stain. MIF immunostaining is seen in basal and intermediate cells and in fibroblasts in the lamina propria of saline treated rats, while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal and intermediate cells of urothelium. CYP treatment resulted in superficial cell staining for MIF and CXCR4 and overlay panels demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar=50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0003898) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-NB100-1789-img0006.jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Daudi (A) and Jurkat (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0003.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NB100-1789] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NB100-1789-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MIF Antibody [NB100-1789] - Co-localization of CXCR4 & MIF in urothelium.Representative sections from rats treated with saline (A–C) or CYP (D–I) are shown. The figure shows MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) & an overlay panel combining both immunostaining & a DAPI nuclear stain. MIF immunostaining is seen in basal & intermediate cells & in fibroblasts in the lamina propria of saline treated rats (A), while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal & intermediate cells of urothelium (B) & lamina propria is not stained. Overlay of these panels (C) demonstrate co-localization of MIF & CXCR4 as orange coloring of cells. CYP treatment resulted in superficial cell staining for MIF (D,G) & CXCR4 (E,H) & overlay panels (F,I) demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar = 50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19066630), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1789_goat-polyclonal-mif-antibody-31020241554531.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0010.jpg "Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: MIF Antibody [NBP1-32192] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32192-img0002.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - Paraffin-embedded mouse brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0012.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate)A: Molt-4 15% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0005.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - : Paraffin-embedded rat brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0011.jpg)
![ELISA: MIF Antibody [NBP1-32192] - ELISA detection of MIF [N1C3] capture at a concentration of 5 ug/mL and MIF C-term antibody for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-ELISA-NBP1-32192-img0008.jpg)