Recombinant Human IL-7 Protein, CF


Recombinant Human IL-7 (207-IL/CF) stimulates proliferation of PHA-activated human peripheral blood lymphocytes in a dose-dependent manner. The ED50 for this effect is 0.1-0.5 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-7 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of Recombinant Human IL-7 is approximately 4.4 x 105 IU/μg, which is calibrated against human IL-7 WHO International Standard (NIBSC code: 90/530).   Specific activity is for reference purposes only and is not routinely tested.  
E. coli-derived human IL-7 protein
Asp26-His177, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
207-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute 5 μg vials at 50 μg/mL in sterile PBS. Reconstitute 10 μg or larger vials at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-7 Protein, CF

  • IL7
  • IL-7
  • IL-7interleukin-7
  • interleukin 7
  • Lymphopoietin-1
  • PBGF


IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1‑4). Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide (3). Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, porcine, feline and canine IL-7. Human and mouse IL-7 exhibit cross-species activity (2, 3). IL-7 protein is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 protein is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha , also known as CD127) with the common gamma chain ( gamma c) (1). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1). IL-7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

  1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
  2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
  4. Namen, A.E. et al. (1988) Nature 333:571.
  5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
  6. Saini, M. et al. (2009) Blood 113:5793.
  7. Park, J.H. et al. (2004) Immunity 21:289.
  8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
  11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
  12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  14. Johnson, K. et al. (2012) J. Immunol. 188:6084.
  15. Nodland, S.E. et al. (2011) Blood 118:2116.

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Publications for IL-7 (207-IL/CF)(143)

We have publications tested in 6 confirmed species: Human, Mouse, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon).

We have publications tested in 7 applications: Bioassay, CAR-T (Bioassay), CAR-T (Differentiation), Cell Culture, ELISA (Standard), In Vivo, Whole Cells.

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CAR-T (Differentiation)
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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
Primate - Papio cynocephalus (Yellow Baboon)
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Showing Publications 1 - 10 of 143. Show All 143 Publications.
Publications using 207-IL/CF Applications Species
P Sekhri, DK Ledezma, A Shukla, EE Sweeney, R Fernandes The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells Cancers, 2022;14(6):. 2022 [PMID: 35326601] (Whole Cells, Human) Whole Cells Human
GR Campbell, J Zhuang, G Zhang, I Landa, LJ Kubiatowic, D Dehaini, RH Fang, L Zhang, SA Spector CD4+ T cell-mimicking nanoparticles encapsulating DIABLO/SMAC mimetics broadly neutralize HIV-1 and selectively kill HIV-1-infected cells Theranostics, 2021;11(18):9009-9021. 2021 [PMID: 34522224] (Bioassay, Human) Bioassay Human
X Yang, X Zhao, Y Zhu, Y Shen, Y Wang, P Lu, Z Jiang, H Pan, J Yang, J Xun, L Zhao, J Wang, Z Liang, X Shen, Y Liang, Q Lin, H Liang, L Jin, D Zhang, J Liu, B Wang, S Jiang, J Xu, H Wu, H Lu, H Zhu FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat MBio, 2021;0(0):e0079521. 2021 [PMID: 34281390] (Bioassay, Human) Bioassay Human
AS Bear, T Blanchard, J Cesare, MJ Ford, LP Richman, C Xu, ML Baroja, S McCuaig, C Costeas, K Gabunia, J Scholler, AD Posey, MH O'Hara, A Smole, DJ Powell, BA Garcia, RH Vonderheid, GP Linette, BM Carreno Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targeting Nature Communications, 2021;12(1):4365. 2021 [PMID: 34272369] (Bioassay, Human) Bioassay Human
J Boustani, ELM Joseph, E Martin, S Benhmida, B Lecoester, F Tochet, C Mirjolet, C Chevalier, D Thibouw, N Vulquin, S Servagi, X Sun, O Adotévi Cisplatin-based chemoradiation decreases telomerase-specific CD4 TH1 response but increases immune suppressive cells in peripheral blood BMC immunology, 2021;22(1):38. 2021 [PMID: 34144673] (Bioassay, Human) Bioassay Human
F Nicoli, S Pacifico, E Gallerani, E Marzola, V Albanese, V Finessi, S Llewellyn-, DA Price, V Appay, P Marconi, R Guerrini, A Caputo, R Gavioli Use of a Novel Peptide Welding Technology Platform for the Development of B- and T-Cell Epitope-Based Vaccines Vaccines, 2021;9(5):. 2021 [PMID: 34069535] (Bioassay, Human) Bioassay Human
S Jung, E Appleton, M Ali, GM Church, A Del Sol A computer-guided design tool to increase the efficiency of cellular conversions Nature Communications, 2021;12(1):1659. 2021 [PMID: 33712564] (Bioassay, Human) Bioassay Human
E Kidzeru, MA Gasper, D Shao, PT Edlefsen, N Lejarcegui, E Havyariman, K Urdahl, S Gantt, H Horton, H Jaspan, A Gervassi Myeloid-derived suppressor cells and their association with vaccine immunogenicity in South African infants Journal of leukocyte biology, 2021;0(0):. 2021 [PMID: 33477200] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox genes HMX2 and HMX3 interferes with cell differentiation in acute myeloid leukemia PLoS ONE, 2020;15(10):e0240120. 2020 [PMID: 33048949] (Cell Culture, Human) Cell Culture Human
M Matsushita, S Saito, S Yokoe, D Ichikawa, Y Hattori Characteristics of a Novel Target Antigen Against Myeloma Cells for Immunotherapy Vaccines (Basel), 2020;8(4):. 2020 [PMID: 33023190] (Bioassay, Human) Bioassay Human
Show All 143 Publications.

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Gene Symbol IL7