Recombinant Human IL-7 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human IL-7 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of Recombinant Human IL-7 is approximately 4.4 x 105 IU/μg, which is calibrated against human IL-7 WHO International Standard (NIBSC code: 90/530).
Source
E. coli-derived human IL-7 protein
Asp26-His177, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL7
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
207-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute 5 μg vials at 50 μg/mL in sterile PBS. Reconstitute 25 μg or larger vials at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-7 Protein, CF

  • IL7
  • IL-7
  • IL-7interleukin-7
  • interleukin 7
  • Lymphopoietin-1
  • PBGF

Background

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1‑4). Human
IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide (3). Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, porcine, feline and canine IL-7.  Human and mouse IL-7 exhibit cross-species activity (2, 3). IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha, also known as CD127) with the common gamma chain ( gamma c) (1). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1). IL-7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

  1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
  2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
  4. Namen, A.E. et al. (1988) Nature 333:571.
  5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
  6. Saini, M. et al. (2009) Blood 113:5793.
  7. Park, J.H. et al. (2004) Immunity 21:289.
  8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
  11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
  12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  14. Johnson, K. et al. (2012) J. Immunol. 188:6084.
  15. Nodland, S.E. et al. (2011) Blood 118:2116.

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207-IL/CF
Species: Hu
Applications: Bioactivity

Publications for IL-7 (207-IL/CF)(102)

We have publications tested in 6 confirmed species: Human, Mouse, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon).

We have publications tested in 4 applications: Bioassay, Cell Culture Substrate, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(92)
Cell Culture Substrate
(1)
ELISA Standard
(4)
In Vivo
(1)
All Applications
Filter By Species
Human
(91)
Mouse
(2)
N/A
(3)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Papio cynocephalus (Yellow Baboon)
(1)
All Species
Showing Publications 1 - 10 of 102. Show All 102 Publications.
Publications using 207-IL/CF Applications Species
S Krishna, P Ulrich, E Wilson, F Parikh, P Narang, S Yang, AK Read, S Kim-Schulz, JG Park, M Posner, MA Wilson Say, A Sikora, KS Anderson Human papilloma virus specific immunogenicity and dysfunction of CD8+ T cells in head and neck cancer Cancer Res., 2018;0(0):. 2018 [PMID: 30154146] (Bioassay, Human) Bioassay Human
SR Kim, JM Lewis, BM Cyrenne, PF Monico, FN Mirza, KR Carlson, FM Foss, M Girardi BET inhibition in advanced cutaneous T cell lymphoma is synergistically potentiated by BCL2 inhibition or HDAC inhibition Oncotarget, 2018;9(49):29193-29207. 2018 [PMID: 30018745] (Bioassay, Human) Bioassay Human
X Zhao, S Sankaran, J Yap, CT Too, ZZ Ho, G Dolton, M Legut, EC Ren, AK Sewell, A Bertoletti, PA MacAry, J Brzostek, NRJ Gascoigne Nonstimulatory peptide-MHC enhances human T-cell antigen-specific responses by amplifying proximal TCR signaling Nat Commun, 2018;9(1):2716. 2018 [PMID: 30006605] (Bioassay, Human) Bioassay Human
LK Thampy, KE Remy, AH Walton, Z Hong, K Liu, R Liu, V Yi, CD Burnham, RS Hotchkiss Restoration of T Cell function in multi-drug resistant bacterial sepsis after interleukin-7, anti-PD-L1, and OX-40 administration PLoS ONE, 2018;13(6):e0199497. 2018 [PMID: 29944697] (Bioassay, Human) Bioassay Human
DJHF Knapp, CA Hammond, T Hui, MTJ van Loenho, F Wang, N Aghaeepour, PH Miller, M Moksa, GM Rabu, PA Beer, D Pellacani, RK Humphries, C Hansen, M Hirst, CJ Eaves Single-cell analysis identifies a CD33+ subset of human cord blood cells with high regenerative potential Nat. Cell Biol., 2018;20(6):710-720. 2018 [PMID: 29802403] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Human) Human
T Kato, T Matsuda, Y Ikeda, JH Park, M Leisegang, S Yoshimura, T Hikichi, M Harada, M Zewde, S Sato, K Hasegawa, K Kiyotani, Y Nakamura Effective screening of T cells recognizing neoantigens and construction of T-cell receptor-engineered T cells Oncotarget, 2018;9(13):11009-11019. 2018 [PMID: 29541393] (Bioassay, Human) Bioassay Human
U Kulkarni, C Herrmenau, SJ Win, M Bauer, T Kamradt IL-7 treatment augments and prolongs sepsis-induced expansion of IL-10-producing B lymphocytes and myeloid-derived suppressor cells PLoS ONE, 2018;13(2):e0192304. 2018 [PMID: 29466409] (In Vivo, Mouse) In Vivo Mouse
S Miyamoto, V Kochin, T Kanaseki, A Hongo, S Tokita, Y Kikuchi, A Takaya, Y Hirohashi, T Tsukahara, T Terui, K Ishitani, F Hata, I Takemasa, A Miyazaki, H Hiratsuka, N Sato, T Torigoe The antigen ASB4 on cancer stem cells serves as a target for CTL immunotherapy of colorectal cancer Cancer Immunol Res, 2018;0(0):. 2018 [PMID: 29371260] (Bioassay, Human) Bioassay Human
M Bardet, A Unterreine, C Malinverni, F Lafossas, C Vedrine, D Boesch, Y Kolb, D Kaiser, A Glück, MA Schneider, A Katopodis, M Renatus, O Simic, A Schlapbach, J Quancard, CH Régnier, G Bold, C Pissot-Sol, JM Carballido, J Kovarik, T Calzascia, F Bornancin The T-cell fingerprint of MALT1 paracaspase revealed by selective inhibition Immunol. Cell Biol., 2018;96(1):81-99. 2018 [PMID: 29359407] (Bioassay, Human) Bioassay Human
Show All 102 Publications.

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Bioinformatics

Gene Symbol IL7
Uniprot