Recombinant Human IL-7 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human IL-7 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of Recombinant Human IL-7 is approximately 4.4 x 105 IU/μg, which is calibrated against human IL-7 WHO International Standard (NIBSC code: 90/530).
Source
E. coli-derived
Asp26-His177, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL7
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
207-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute 5 μg vials at 50 μg/mL in sterile PBS. Reconstitute 25 μg or larger vials at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-7 Protein, CF

  • IL7
  • IL-7
  • IL-7interleukin-7
  • interleukin 7
  • Lymphopoietin-1
  • PBGF

Background

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1‑4). Human
IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide (3). Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, porcine, feline and canine IL-7.  Human and mouse IL-7 exhibit cross-species activity (2, 3). IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha, also known as CD127) with the common gamma chain ( gamma c) (1). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1). IL-7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

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Publications for (84)

We have publications tested in 6 confirmed species: Human, Mouse, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon).

We have publications tested in 3 applications: Bioassay, Cell Culture Substrate, ELISA Standard.


Filter By Application
Bioassay
(76)
Cell Culture Substrate
(1)
ELISA Standard
(4)
All Applications
Filter By Species
Human
(74)
Mouse
(1)
N/A
(3)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Papio cynocephalus (Yellow Baboon)
(1)
All Species
Showing Publications 1 - 10 of 84. Show All 84 Publications.
Publications using 207-IL/CF Applications Species
M Yoshida, Y Ishioka, T Ozawa, H Okuyama, M Iguchi, T Ota, T Ito, M Nagira, A Morita, H Tanaka, H Naito, H Kidoya, N Takakura Soluble HLA-associated peptide from PSF1 has a cancer vaccine potency Sci Rep, 2017;7(1):11137. 2017 [PMID: 28894200] (Bioassay, Human) Bioassay Human
CA Ramos, B Ballard, H Zhang, O Dakhova, AP Gee, Z Mei, M Bilgi, MF Wu, H Liu, B Grilley, CM Bollard, BH Chang, CM Rooney, MK Brenner, HE Heslop, G Dotti, B Savoldo Clinical and immunological responses after CD30-specific chimeric antigen receptor-redirected lymphocytes J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28805662] (Bioassay, Human) Bioassay Human
F Venet, J Demaret, BJ Blaise, C Rouget, T Girardot, E Idealisoa, T Rimmelé, F Mallet, A Lepape, J Textoris, G Monneret IL-7 Restores T Lymphocyte Immunometabolic Failure in Septic Shock Patients through mTOR Activation J. Immunol., 2017;0(0):. 2017 [PMID: 28724580] (Bioassay, Human) Bioassay Human
J Ding, ML Fishel, AM Reed, E McAdams, M Czader, AA Cardoso, MR Kelley Ref-1/APE1 as Transcriptional Regulator and Novel Therapeutic Target in Pediatric T-cell Leukemia Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28446640] (Bioassay, Human) Bioassay Human
S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 2017;14(5):531-538. 2017 [PMID: 28394335] (Bioassay, Human) Bioassay Human
T Koya, R Yanagisawa, Y Higuchi, K Sano, S Shimodaira Interferon-?-inducible Dendritic Cells Matured with OK-432 Exhibit TRAIL and Fas Ligand Pathway-mediated Killer Activity Sci Rep, 2017;7(0):42145. 2017 [PMID: 28191816] (Bioassay, Human) Bioassay Human
L Turtle, F Tatullo, T Bali, V Ravi, M Soni, S Chan, S Chib, MM Venkataswa, P Fadnis, M Ya‹ch, S Fernandez, P Klenerman, V Satchidana, T Solomon Cellular Immune Responses to Live Attenuated Japanese Encephalitis (JE) Vaccine SA14-14-2 in Adults in a JE/Dengue Co-Endemic Area PLoS Negl Trop Dis, 2017;11(1):e0005263. 2017 [PMID: 28135273] (Bioassay, Human) Bioassay Human
Human Cytomegalovirus miR-UL148D Facilitates Latent Viral Infection by Targeting Host Cell Immediate Early Response Gene 5 PLoS Pathog., 2016;12(11):e1006007. 2016 [PMID: 27824944] (Bioassay, Human) Bioassay Human
Interleukin-7 Plasma Levels in Human Differentiate Anorexia Nervosa, Constitutional Thinness and Healthy Obesity PLoS ONE, 2016;11(9):e0161890. 2016 [PMID: 27611669] (ELISA Standard, Human) ELISA Standard Human
Identification of a novel human memory T-cell population with the characteristics of stem-like chemo-resistance Oncoimmunology, 2016;5(6):e1165376. 2016 [PMID: 27471640] (Bioassay, Human) Bioassay Human
Show All 84 Publications.

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Bioinformatics

Gene Symbol IL7
Entrez
Uniprot