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207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-7 Monoclonal Antibody (Catalog # MAB207). The ND50 is typically 0.4-0.8 µg/mL." class="big_lightbox" target="_blank">
207-IL) was serially diluted and captured by Mouse Anti-Human IL‑7 Monoclonal Antibody (Catalog # MAB207) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407). The ND50 is typically 0.2-0.4 µg/mL." class="big_lightbox" target="_blank">
407-ML) was serially diluted and captured by Goat Anti-Mouse IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
407-ML) was serially diluted and captured by Goat Anti-Mouse IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
207-IL), Recombinant Mouse IL-7 (407-ML) and Recombinant Rat IL-7 (7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF109). A specific band was detected for IL-7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">
207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND50 is typically ≤ 0.5µg/mL." class="big_lightbox" target="_blank">
207-IL) was serially diluted and captured by Mouse Anti-Human IL‑7 Monoclonal Antibody (Catalog # MAB207) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Interleukin-7 (IL-7, previously known as lymphopoietin-1 and pre-B-cell growth factor) is a hematopoietic growth factor that stimulates early B and T cells. It can also co-stimulate the proliferation of mature T cells with other factors such as ConA (concanavalin A) and IL-2. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells, it also plays a role in the development of cytotoxic T lymphocytes (CTL). IL-7 can stimulate the production of pro-inflammatory cytokines and enhance the tumoricidal activity of monocytes/macrophages. It is produced by thymic stromal cells, spleen cells and keratinocytes. Human and murine IL-7 show cross-species reactivity.
![Neutralization IL-7 Antibody (7417) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-7_MAB207_Block_Neutralize_9062.jpg)
![Western Blot IL-7 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL7_AF207NA_Western_Blot_21509.jpg)
![Sandwich ELISA IL-7 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-7-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79370-img0001.jpg)
![N/A IL-7 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL7_HS750_ELISA_890.jpg)
![N/A IL-7 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL7_DY207_ELISA_1763.jpg)
![N/A IL-7 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL7_M7000_ELISA_937.jpg)
![SEC-MALS IL-7 [Unconjugated]](http://images.novusbio.com/fullsize/207-il_recombinant-human-il-7-protein-sec-mals-123202493447..jpg)
![SEC-MALS IL-7 [Unconjugated]](http://images.novusbio.com/fullsize/407-ml_recombinant-mouse-il-7-protein-sec-mals-1811202515316.jpg)
![ELISA IL-7 Antibody (7417) [Unconjugated]](http://images.novusbio.com/images/mab207_human-il-7-mab-clone-7417-elisa-639201776107752610.jpg)
![Neutralization IL-7 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-7_AF-207-NA_Block_Neutralize_8645.jpg)
![ELISA IL-7 Antibody [Unconjugated]](http://images.novusbio.com/images/af-207-na_human-il-7-affinity-purified-polyclonal-ab-elisa-639201852922459657.jpg)
![N/A IL-7 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL7_HS750_ELISA_891.jpg)
![N/A IL-7 [HRP]](http://images.novusbio.com/fullsize/elisa/IL-7_HS750_ELISA_400.jpg)
![N/A IL-7 [HRP]](http://images.novusbio.com/fullsize/elisa/IL-7_M7000_ELISA_238.jpg)
![Bioactivity IL-7 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-7_407-ML_539.jpg)
![SDS-Page IL-7 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-7_407-ML_537.jpg)
