207-IL), Recombinant Mouse IL-7 (
407-ML) and Recombinant Rat IL-7 (
7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody
HAF109). A specific band was detected for IL-7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_lightbox">

207-IL), Recombinant Mouse IL-7 (
407-ML) and Recombinant Rat IL-7 (
7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody
HAF109). A specific band was detected for IL-7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human IL-7 (
207-IL), Recombinant Mouse IL-7 (
407-ML) and Recombinant Rat IL-7 (
7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody
HAF109). A specific band was detected for IL-7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_thumb" />
207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND
50 is typically ≤ 0.5µg/mL." class="big_lightbox">

207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND
50 is typically ≤ 0.5µg/mL." class="big_thumb" />
207-IL) was serially diluted and captured by Mouse Anti-Human IL‑7 Monoclonal Antibody (Catalog #
MAB207) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

207-IL) was serially diluted and captured by Mouse Anti-Human IL‑7 Monoclonal Antibody (Catalog #
MAB207) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />