Recombinant Human Follistatin 300 Protein

Images

 
There are currently no images for Follistatin (669-FO).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Follistatin 300 Protein Summary

Details of Functionality
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 7.5 ng/mL of rhActivin A.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Follistatin protein
Gly30-Asp329, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
FST
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40 kDa, reducing conditions
Publications
Read Publications using
669-FO in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Follistatin 300 Protein

  • follistatin isoform FST317
  • Follistatin
  • FS
  • FSActivin-binding protein
  • FST

Background

Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high‑affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS‑288) and 315 aa residues (FS‑315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS‑315 composed of 300 aa residues. The recombinant human FS‑300 produced at R&D Systems contains 301 aa residues and represents a molecular form derived from human FS‑315 containing a truncation of 15 residues from the carboxy–terminus. FS‑288 binds with high‑affinity to cell-surface heparan sulfate proteoglycans whereas FS‑315 binds with low‑affinity. The binding affinity of R&D Systems' FS‑300 to heparan sulfate has not been determined. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Over‑expression of FS can also cause reproductive defects in transgenic mice.

  1. Iemura, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9337
  2. Guo, Q. (1998) Mol. Endocrinol. 12:96
  3. Hashimoto, O. et al. (1997) J. Biol. Chem. 272:13835

Customers Who Viewed This Item Also Viewed...

NBP1-84310
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87147
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-38770
Species: Hu, Mu
Applications: WB, IHC, IHC-P
248-BD
Species: Hu, Mu, Rt, Ca, Eq
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP2-43648
Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Follistatin (669-FO)(12)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Goat, Xenopus.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(10)
In Vivo
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
Rat
(2)
Bovine
(1)
Chicken
(1)
Goat
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 669-FO Applications Species
J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 2018;0(0):9636897187803. 2018 [PMID: 29895168] (Bioassay, Human) Bioassay Human
J Hu, X Wang, YH Tang, YG Shan, Q Zou, ZQ Wang, CX Huang Activin A inhibition attenuates sympathetic neural remodeling following myocardial infarction in rats Mol Med Rep, 2018;17(4):5074-5080. 2018 [PMID: 29393433] (In Vivo, Rat) In Vivo Rat
M Ashry, SK Rajput, JK Folger, JG Knott, NA Hemeida, OM Kandil, RS Ragab, GW Smith Functional role of AKT signaling in bovine early embryonic development: potential link to embryotrophic actions of follistatin Reprod. Biol. Endocrinol., 2018;16(1):1. 2018 [PMID: 29310676] (Bioassay, Bovine) Bioassay Bovine
DD Seachrist, ST Sizemore, E Johnson, FW Abdul-Kari, KL Weber Bonk, RA Keri Follistatin is a metastasis suppressor in a mouse model of HER2-positive breast cancer Breast Cancer Res., 2017;19(1):66. 2017 [PMID: 28583174] (Bioassay, Human) Bioassay Human
Yoshihisa Nojima Involvement of Infiltrating Macrophage-derived Activin A in the Progression of Renal Damage in MRL-lpr Mice Am. J. Physiol. Renal Physiol., 2016;0(0):ajprenal.0019. 2016 [PMID: 27881396] (In Vivo, Mouse) In Vivo Mouse
Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling. Blood, 2008;112(8):3154-63. 2008 [PMID: 18664625] (Bioassay, Human) Bioassay Human
Alexander S, Watt F, Sawaji Y, Hermansson M, Saklatvala J Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB. Arthritis Rheum., 2007;56(11):3715-25. 2007 [PMID: 17968943] (Bioassay, Human) Bioassay Human
Wen Z, Han L, Bamburg JR, Shim S, Ming GL, Zheng JQ BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin. J. Cell Biol., 2007;178(1):107-19. 2007 [PMID: 17606869] (Bioassay, Xenopus) Bioassay Xenopus
McLean AB, D&amp;amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 2007;25(1):29-38. 2007 [PMID: 17204604] (Bioassay, Human) Bioassay Human
Silva JR, Tharasanit T, Taverne MA, van der Weijden GC, Santos RR, Figueiredo JR, van den Hurk R The activin-follistatin system and in vitro early follicle development in goats. J. Endocrinol., 2006;189(1):113-25. 2006 [PMID: 16614386] (Bioassay, Goat) Bioassay Goat
Show All 12 Publications.

Reviews for Follistatin (669-FO) (0)

There are no reviews for Follistatin (669-FO). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Follistatin (669-FO) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Follistatin (669-FO)

Discover related pathways, diseases and genes to Follistatin (669-FO). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Follistatin (669-FO)

Discover more about diseases related to Follistatin (669-FO).
 

Pathways for Follistatin (669-FO)

View related products by pathway.

PTMs for Follistatin (669-FO)

Learn more about PTMs related to Follistatin (669-FO).

Blogs on Follistatin

There are no specific blogs for Follistatin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Albumin Antibody
NB600-41532

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Follistatin 300 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol FST
Uniprot