Recombinant Human Follistatin 288 (FS-288) Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Follistatin 288 (FS-288) Protein Summary

Details of Functionality
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells.

The ED50 for this effect is 0.05‑0.25 μg/mL in the presence of 7.5 ng/mL of rhActivin A.

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Follistatin protein
Gly30-Asn317, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
FST
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35-40 kDa, reducing conditions
Publications
Read Publications using
5836-FS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Follistatin 288 (FS-288) Protein

  • follistatin isoform FST317
  • Follistatin
  • FS
  • FSActivin-binding protein
  • FST

Background

Follistatin 288 (FST288) is one of three forms of follistatin, a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human follistatin 288 cDNA encodes a 317 amino acid (aa) protein with a 29 aa signal sequence, and a 288 aa mature region. Human FST288 shares 97 - 99% aa identity with corresponding regions of mouse, rat, equine, ovine, porcine and bovine FST. All forms of follistatin, including FST288, FST303 and FST315, contain an N-terminal atypical TGF binding domain and three follistatin domains (FS1 - 3) that contain EGF-like and kazal-like motifs. A highly acidic C-terminal tail is missing in the splice variant FST288, partially present in the proteolytically produced FST303, and full-length in the most abundant form, FST315 (5, 8, 9). FSTs inhibit activins by surrounding activin dimers. FST288 shows the highest affinity for activins due to its extended configuration, while FST315 is in a folded form (2, 8). Expression of the isoforms is restricted such that FST288, which has higher affinity for heparin-sulfated proteoglycans when unbound, is present in tissues while FST315 is the sole form in plasma and ovarian follicular fluid (5, 8, 9). In addition to activin, follistatins regulate the bioavailability of other members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). Follistatins also regulate hematopoietic stem cell adhesion to fibronectin via FS2, and bind angiogenin via FS2 and FS3 (6, 7). Genetic deletion of follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Mice that express only the FST315 isoform survive, but exhibit defects in vascularization and female fertility (10).

  1. Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218. 
  2. Thompson, T.B. et al. (2005) Dev. Cell 9:535. 
  3. Sidis, Y. et al. (2005) Endocrinology 146:130. 
  4. Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228. 
  5. Sidis, Y. et al. (2006) Endocrinology 147:3586. 
  6. Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
  7. Gao, X. et al. (2007) FEBS Lett. 581:5505.
  8. Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
  9. Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
  10. Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.

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Publications for Follistatin (5836-FS)(5)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 1 application: Bioassay.


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(5)
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Bioinformatics

Gene Symbol FST
Uniprot