Recombinant Human FGF-4 Protein, CF

Recombinant Human FGF-4 (Catalog # 235‑F4/CF) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.25‑1.25 ng/mL.

1 μg/lane of Recombinant Human FGF-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human FGF-4 Protein, CF Summary

• Additional Information: A New and Improved rh FGF-4 is Now Available! It has enhanced purity!
• Details of Functionality: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED 50 for this effect is 0.25-1.25 ng/mL.
• Source: E. coli-derived human FGF-4 protein Ser54-Leu206
• Accession #: P08620
• N-terminal Sequence: Ser54 & Leu55
• Protein/Peptide Type: Recombinant Proteins
• Gene: FGF4
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 16 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-4 Protein, CF

• FGF4
• FGF-4
• fibroblast growth factor 4
• HBGF-4
• HBGF-4Transforming protein KS3
• heparin secretory transforming protein 1
• Heparin secretory-transforming protein 1
• Heparin-binding growth factor 4
• HST-1
• HST-1HSTF-1
• HSTF1fibroblast growth factor 4 splice isoform
• HSTFGF-4
• human stomach cancer, transforming factor from FGF-related oncogene
• kaposi sarcoma oncogene
• KFGF
• K-FGF
• KS3
• oncogene HST

Background

FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature human FGF-4 (aa 71-206) shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF-4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the mouse trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3-5). Later in mouse development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6-9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10-12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
3. Niswander, L. and G.R. Martin (1992) Development 114:755.
4. Feldman, B. et al. (1995) Science 267:246.
5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
6. Sun, X. et al. (2002) Nature 418:501.
7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
8. Yu, K and D.M. Ornitz (2008) Development 135:483.
9. Mariani, F.V. et al. (2008) Nature 453:401.
10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
11. Kunath, T. et al. (2007) Development 134:2895.
12. Mayshar, Y. et al. (2008) Stem Cells 26:767.
13. Hajitou, A. et al. (1998) Oncogene 17:2059.
14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.

Publications for FGF-4 (235-F4/CF)(65)

Publications using 235-F4/CF

• A Rössig, K Hill, W Nörenberg, S Weidenbach, S Zierler, M Schaefer, T Gudermann, V Chubanov Pharmacological agents selectively acting on the channel moieties of TRPM6 and TRPM7 Cell Calcium, 2022-08-17;106(0):102640. 2022-08-17 [PMID: 36030694] (Cell Culture, Human)
• J Seong, J Frias-Alde, V Holzmann, H Kagawa, G Sestini, H Heidari Kh, Y Scholte Op, M Kip, SJ Pradhan, L Verwegen, J Vivié, L Li, A Alemany, J Korving, F Darmis, A van Oudena, D Ten Berge, N Geijsen, NC Rivron Epiblast inducers capture mouse trophectoderm stem cells in�vitro and pattern blastoids for implantation in utero Cell Stem Cell, 2022-07-07;29(7):1102-1118.e8. 2022-07-07 [PMID: 35803228] (Bioassay, Mouse)
• J Jeong, TH Kim, M Kim, YK Jung, KS Kim, S Shim, H Jang, WI Jang, SB Lee, D Choi Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids Biology, 2022-03-23;11(4):. 2022-03-23 [PMID: 35453693] (Bioassay, Human)
• S Xia, Z Bozóky, M Di Paola, O Laselva, S Ahmadi, JX Jiang, AL Pitstick, C Jiang, D Rotin, CN Mayhew, NL Jones, CE Bear High-Throughput Functional Analysis of CFTR and Other Apically Localized Proteins in iPSC-Derived Human Intestinal Organoids Cells, 2021-12-04;10(12):. 2021-12-04 [PMID: 34943927] (Bioassay, Human)
• V Chesnokova, S Zonis, A Apostolou, HQ Estrada, S Knott, K Wawrowsky, K Michelsen, A Ben-Shlomo, R Barrett, V Gorbunova, K Karalis, S Melmed Local non-pituitary growth hormone is induced with aging and facilitates epithelial damage Cell Reports, 2021-12-14;37(11):110068. 2021-12-14 [PMID: 34910915] (Bioassay, Human)
• AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells Cell Stem Cell, 2021-12-01;0(0):. 2021-12-01 [PMID: 34856121] (Bioassay, Human)
• MM Ali, SA Khater, AA Fayed, D Sabry, SF Ibrahim Apoptotic endocrinal toxic effects of perchlorate in human placental cells Toxicology reports, 2021-04-17;8(0):863-870. 2021-04-17 [PMID: 33948439] (Cell Culture, Human)
• K Husain, D Coppola, CS Yang, MP Malafa Farnesyl dimethyl chromanol targets colon cancer stem cells and prevents colorectal cancer metastasis Scientific Reports, 2021-01-26;11(1):2185. 2021-01-26 [PMID: 33500430] (Bioassay, Human)
• SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021-01-11;12(1):262. 2021-01-11 [PMID: 33431859] (Bioassay, Human)
• E Posfai, JP Schell, A Janiszewsk, I Rovic, A Murray, B Bradshaw, T Yamakawa, T Pardon, M El Bakkali, I Talon, N De Geest, P Kumar, SK To, S Petropoulo, A Jurisicova, V Pasque, F Lanner, J Rossant Evaluating totipotency using criteria of increasing stringency Nature Cell Biology, 2021-01-08;23(1):49-60. 2021-01-08 [PMID: 33420491] (Bioassay, Transgenic Mouse)
• H Fang, S Geng, M Hao, Q Chen, M Liu, C Liu, Z Tian, C Wang, T Takebe, JL Guan, Y Chen, Z Guo, W He, J Diao Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells Nature Communications, 2021-01-04;12(1):109. 2021-01-04 [PMID: 33397937] (Bioassay, Human)
• C Sangokoya, R Blelloch MicroRNA-dependent inhibition of PFN2 orchestrates ERK activation and pluripotent state transitions by regulating endocytosis Proc. Natl. Acad. Sci. U.S.A., 2020-08-11;117(34):20625-20635. 2020-08-11 [PMID: 32788350] (Bioassay, Human, Mouse)
• S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 2020-04-06;10(1):5989. 2020-04-06 [PMID: 32249832] (Bioassay, Human)
• MJ Jeyarajah, G Jaju Bhatt, DM Hillier, SJ Renaud The Transcription Factor OVOL2 Represses ID2 and Drives Differentiation of Trophoblast Stem Cells and Placental Development in Mice Cells, 2020-03-31;9(4):. 2020-03-31 [PMID: 32244352] (Bioassay, Mouse)
• Y Toba, S Deguchi, N Mimura, A Sakamoto, K Harada, K Hirata, K Takayama, H Mizuguchi Comparison of commercially available media for hepatic differentiation and hepatocyte maintenance PLoS ONE, 2020-02-27;15(2):e0229654. 2020-02-27 [PMID: 32106262] (Cell Culture, Human)
• NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094658] (Cell Culture, Human)
• JS Kim, SI Hwang, JL Ryu, HS Hong, JM Lee, SM Lee, X Jin, C Han, JH Kim, J Han, MR Lee, DH Woo ER stress reliever enhances functionalities of in vitro cultured hepatocytes Stem Cell Res, 2020-02-12;43(0):101732. 2020-02-12 [PMID: 32087526] (Bioassay, Human)
• Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020-02-17;21(3):274-286. 2020-02-17 [PMID: 32066947] (Cell Culture, Human)
• H Zhang, LTP Khoa, F Mao, H Xu, B Zhou, Y Han, M O'Leary, A Nusrat, L Wang, TL Saunders, Y Dou MLL1 Inhibition and Vitamin D Signaling Cooperate to Facilitate the Expanded Pluripotency State Cell Rep, 2019-11-26;29(9):2659-2671.e6. 2019-11-26 [PMID: 31775036] (Cell Culture, Mouse)
• KB Jung, O Kwon, MO Lee, H Lee, YS Son, O Habib, JH Oh, HS Cho, CR Jung, J Kim, MY Son Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells J Clin Med, 2019-07-04;8(7):. 2019-07-04 [PMID: 31277507] (Bioassay, Human)
• RMJ Genga, EM Kernfeld, KM Parsi, TJ Parsons, MJ Ziller, R Maehr Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development Cell Rep, 2019-04-16;27(3):708-718.e10. 2019-04-16 [PMID: 30995470] (Bioassay, Human)
• Y Toba, A Kiso, S Nakamae, F Sakurai, K Takayama, H Mizuguchi FGF signal is not required for hepatoblast differentiation of human iPS cells Sci Rep, 2019-03-06;9(1):3713. 2019-03-06 [PMID: 30842525] (Bioassay, Human)
• V Chesnokova, S Zonis, R Barrett, H Kameda, K Wawrowsky, A Ben-Shlomo, M Yamamoto, J Gleeson, C Bresee, V Gorbunova, S Melmed Excess growth hormone suppresses DNA damage repair in epithelial cells JCI Insight, 2019-02-07;4(3):. 2019-02-07 [PMID: 30728323] (Bioassay, Human)
• TR Broda, KW McCracken, JM Wells Generation of human antral and fundic gastric organoids from pluripotent stem cells Nat Protoc, 2019-01-01;14(1):28-50. 2019-01-01 [PMID: 30470820] (Bioassay, Human)
• A Hyder, S Ehnert, F Fändrich, H Ungefroren Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells Stem Cells Int, 2018-10-04;2018(0):4271875. 2018-10-04 [PMID: 30402109] (Bioassay, Human)
• B Wang, JH Joo, R Mount, BJW Teubner, A Krenzer, AL Ward, VP Ichhaporia, EJ Adams, R Khoriaty, ST Peters, SM Pruett-Mil, SS Zakharenko, D Ginsburg, M Kundu The COPII cargo adapter SEC24C is essential for neuronal homeostasis J. Clin. Invest., 2018-06-25;0(0):. 2018-06-25 [PMID: 29939162] (Bioassay, Human)
• Michael J Workman, John P Gleeson, Elissa J Troisi, Hannah Q Estrada, S Jordan Kerns, Christopher D Hinojosa, Geraldine A Hamilton, Stephan R Targan, Clive N Svendsen, Robert J Barrett Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips. Cellular and Molecular Gastroenterology and Hepatology, 2017-12-29;0(0):2352-345X. 2017-12-29 [PMID: 29930984] (Bioassay, Human)
• J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 2018-06-13;0(0):9636897187803. 2018-06-13 [PMID: 29895168] (Bioassay, Human)
• R Okamoto, K Takayama, N Akita, Y Nagamoto, D Hosokawa, S Iizuka, F Sakurai, H Suemizu, K Ohashi, H Mizuguchi Human iPS Cell-based Liver-like Tissue Engineering at Extrahepatic Sites in Mice as a New Cell Therapy for Hemophilia B Cell Transplant, 2018-02-01;27(2):299-309. 2018-02-01 [PMID: 29637813] (Bioassay, Human)
• WS Fagg, N Liu, MJ Yang, K Cheng, E Chung, JS Kim, G Wu, J Fair Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver Cell Transplant, 2017-12-01;26(12):1868-1877. 2017-12-01 [PMID: 29390880] (Bioassay, Mouse)
• JO Múnera, N Sundaram, SA Rankin, D Hill, C Watson, M Mahe, JE Vallance, NF Shroyer, KL Sinagoga, A Zarzoso-La, JR Hudson, JC Howell, P Chatuvedi, JR Spence, JM Shannon, AM Zorn, MA Helmrath, JM Wells Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling Cell Stem Cell, 2017-06-22;0(0):. 2017-06-22 [PMID: 28648364] (Bioassay, Human)
• M Kang, V Garg, AK Hadjantona Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2 Dev. Cell, 2017-05-25;0(0):. 2017-05-25 [PMID: 28552559] (Bioassay, Mouse)
• S Mitani, K Takayama, Y Nagamoto, K Imagawa, F Sakurai, M Tachibana, R Sumazaki, H Mizuguchi Human ESC/iPSC-Derived Hepatocyte-like Cells Achieve Zone-Specific Hepatic Properties by Modulation of WNT Signaling Mol. Ther., 2017-04-24;25(6):1420-1433. 2017-04-24 [PMID: 28462819] (Bioassay, Human)
• AB Hanker, JG Garrett, MV Estrada, PD Moore, PG Ericsson, JP Koch, E Langley, S Singh, PS Kim, GM Frampton, EM Sanford, P Owns, J Becker, MR Groseclose, S Castellino, H Joensuu, J Huober, JC Brase, M Samira, S Brohée, D Venet, D Brown, J Baselga, M Piccart, C Sotiriou, CL Arteaga HER2-overexpressing breast cancers amplify FGFR signaling upon acquisition of resistance to dual therapeutic blockade of HER2 Clin. Cancer Res., 2017-04-05;0(0):. 2017-04-05 [PMID: 28381415] (Bioassay, Human)
• DA Elberry, SN Amin, RS Esmail, LA Rashed, MM Gamal Effect of undifferentiated versus hepatogenic partially differentiated mesenchymal stem cells on hepatic and cognitive functions in liver cirrhosis EXCLI J, 2016-11-07;15(0):652-670. 2016-11-07 [PMID: 28337098] (Bioassay, Rat)
• DB Holmes, VM Heine Simplified 3D protocol capable of generating early cortical neuroepithelium Biol Open, 2017-03-15;0(0):. 2017-03-15 [PMID: 28167491] (Bioassay, Human)
• KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells Wnt/?-catenin promotes gastric fundus specification in mice and humans Nature, 2017-01-04;0(0):. 2017-01-04 [PMID: 28052057] (Bioassay, Human)
• Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst Nat Commun, 2016-11-18;7(0):13463. 2016-11-18 [PMID: 27857135] (Bioassay, Mouse)
• Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 2016-05-25;0(0):. 2016-05-25 [PMID: 27226307] (Bioassay, Human)
• Morrison G, Scognamiglio R, Trumpp A, Smith A Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification. EMBO J, 2015-12-16;35(3):356-68. 2015-12-16 [PMID: 26675138] (Bioassay, Mouse)
• Tamminen K, Balboa D, Toivonen S, Pakarinen M, Wiener Z, Alitalo K, Otonkoski T Intestinal Commitment and Maturation of Human Pluripotent Stem Cells Is Independent of Exogenous FGF4 and R-spondin1. PLoS ONE, 2015-07-31;10(7):e0134551. 2015-07-31 [PMID: 26230325] (Bioassay, Human)
• Dye , Briana R, Hill , David R, Ferguson , Michael, Tsai , Yu-Hwai, Nagy , Melinda, Dyal , Rachel, Wells , James M, Mayhew , Christop, Nattiv , Roy, Klein , Ophir D, White , Eric S, Deutsch , Gail H, Spence , Jason R In vitro generation of human pluripotent stem cell derived lung organoids. Elife, 2015-03-24;4(0):. 2015-03-24 [PMID: 25803487] (Bioassay, Human)
• Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta. J Virol, 2015-03-04;89(9):5134-47. 2015-03-04 [PMID: 25741001] (Bioassay, Human)
• Krawchuk D, Anani S, Honma-Yamanaka N, Polito S, Shafik M, Yamanaka Y Loss of LKB1 leads to impaired epithelial integrity and cell extrusion in the early mouse embryo. J Cell Sci, 2015-01-14;128(5):1011-22. 2015-01-14 [PMID: 25588837] (Bioassay, Mouse)
• Liu D, Yovchev M, Zhang J, Alfieri A, Tchaikovskaya T, Laconi E, Dabeva M Identification and characterization of mesenchymal-epithelial progenitor-like cells in normal and injured rat liver. Am J Pathol, 2014-11-06;185(1):110-28. 2014-11-06 [PMID: 25447047] (Bioassay, Rat)
• Leslie J, Huang S, Opp J, Nagy M, Kobayashi M, Young V, Spence J Persistence and toxin production by Clostridium difficile within human intestinal organoids result in disruption of epithelial paracellular barrier function. Infect Immun, 2014-10-13;83(1):138-45. 2014-10-13 [PMID: 25312952] (Bioassay, Human)
• Maclary E, Buttigieg E, Hinten M, Gayen S, Harris C, Sarkar M, Purushothaman S, Kalantry S Differentiation-dependent requirement of Tsix long non-coding RNA in imprinted X-chromosome inactivation. Nat Commun, 2014-06-30;5(0):4209. 2014-06-30 [PMID: 24979243] (Bioassay, Mouse)
• Watanabe , Hitoshi, Takayama , Kazuo, Inamura , Mitsuru, Tachibana , Masashi, Mimura , Natsumi, Katayama , Kazufumi, Tashiro , Katsuhis, Nagamoto , Yasuhito, Sakurai , Fuminori, Kawabata , Kenji, Furue , Miho Kus, Mizuguchi , Hiroyuki HHEX promotes hepatic-lineage specification through the negative regulation of eomesodermin. PLoS ONE, 2014-03-20;9(3):e90791. 2014-03-20 [PMID: 24651531] (Bioassay, Human)
• Lam A, Freedman B, Morizane R, Lerou P, Valerius M, Bonventre J Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers. J Am Soc Nephrol, 2013-12-19;25(6):1211-25. 2013-12-19 [PMID: 24357672] (Bioassay, Human)
• Takayama K, Nagamoto Y, Mimura N, Tashiro K, Sakurai F, Tachibana M, Hayakawa T, Kawabata K, Mizuguchi H Long-term self-renewal of human ES/iPS-derived hepatoblast-like cells on human laminin 111-coated dishes. Stem Cell Reports, 2013-10-03;1(4):322-35. 2013-10-03 [PMID: 24319667] (Bioassay, Human)
• Takayama K, Kawabata K, Nagamoto Y, Inamura M, Ohashi K, Okuno H, Yamaguchi T, Tashiro K, Sakurai F, Hayakawa T, Okano T, Furue M, Mizuguchi H CCAAT/enhancer binding protein-mediated regulation of TGFbeta receptor 2 expression determines the hepatoblast fate decision. Development, 2013-11-27;141(1):91-100. 2013-11-27 [PMID: 24284203] (Bioassay, Human)
• Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S Derivation of cerebellar neurons from human pluripotent stem cells. Curr Protoc Stem Cell Biol, 2012-03-01;20(0):1H.51. 2012-03-01 [PMID: 22415839] (Bioassay, Human)
• Wang P, McKnight K, Wong D, Rodriguez R, Sugiyama T, Gu X, Ghodasara A, Qu K, Chang H, Kim S A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells. Stem Cells Dev, 2012-02-29;21(12):2273-87. 2012-02-29 [PMID: 22236333] (Bioassay, Human, Mouse)
• Tomlinson DC, Knowles MA Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer. Am. J. Pathol., 2010-10-01;177(5):2379-86. 2010-10-01 [PMID: 20889570] (Bioassay, Human)
• Roelandt P, Pauwelyn KA, Sancho-Bru P Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells. PLoS ONE, 2010-08-11;5(8):e12101. 2010-08-11 [PMID: 20711405] (Cell Culture, Human)
• Self-regulation of Stat3 activity coordinates cell-cycle progression and neural crest specification. EMBO J., 2009-10-22;29(1):55-67. 2009-10-22 [PMID: 19851287] (Bioassay, Xenopus)
• Sugiura K, Su YQ, Li Q, Wigglesworth K, Matzuk MM, Eppig JJ Fibroblast growth factors and epidermal growth factor cooperate with oocyte-derived members of the TGFbeta superfamily to regulate Spry2 mRNA levels in mouse cumulus cells. Biol. Reprod., 2009-06-24;81(5):833-41. 2009-06-24 [PMID: 19553596] (Bioassay, Mouse)
• Salero E, Hatten ME Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. U.S.A., 2007-02-09;104(8):2997-3002. 2007-02-09 [PMID: 17293457] (Bioassay, Mouse)
• Williamson D, Selfe J, Lu YJ, Pritchard-Jones K, Murai K, Jones P, Workman P, Shipley J Role for amplification and expression of glypican-5 in rhabdomyosarcoma. Cancer Res., 2007-01-01;67(1):57-65. 2007-01-01 [PMID: 17210683] (Bioassay, Xenopus)
• Nakayama T, Mutsuga N, Tosato G FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc. Blood, 2006-10-31;109(4):1363-72. 2006-10-31 [PMID: 17077327] (Bioassay, Mouse)
• Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, O'Brien TD, Zhang J, Verfaillie C Multipotent adult progenitor cells from swine bone marrow. Stem Cells, 2006-08-24;24(11):2355-66. 2006-08-24 [PMID: 16931778] (Bioassay, Porcine)
• Tiecke E, Bangs F, Blaschke R, Farrell ER, Rappold G, Tickle C Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements. Dev. Biol., 2006-07-12;298(2):585-96. 2006-07-12 [PMID: 16904661] (In Vivo, Chicken)
• Nordstrom U, Maier E, Jessell TM, Edlund T An early role for WNT signaling in specifying neural patterns of Cdx and Hox gene expression and motor neuron subtype identity. PLoS Biol., 2006-07-01;4(8):e252. 2006-07-01 [PMID: 16895440] (Bioassay, Chicken)
• Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis. Development, 2005-02-16;132(6):1223-34. 2005-02-16 [PMID: 15716343] (Bioassay, Mouse)
• Shimon I, Huttner A, Said J, Spirina OM, Melmed S Heparin-binding secretory transforming gene (hst) facilitates rat lactotrope cell tumorigenesis and induces prolactin gene transcription. J. Clin. Invest., 1996-01-01;97(1):187-95. 1996-01-01 [PMID: 8550832] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: FGF4
• Uniprot:
  • P08620()