Recombinant Human CD117/c-kit Fc Chimera Protein, CF

Recombinant Human CD117/c-kit Fc Chimera Protein (Catalog # 10961-SR) inhibits SCF-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 30.0-180 ng/mL.

2 μg/lane of Recombinant Human CD117/c-kit Fc Chimera Protein (Catalog # 10961-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200-240 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD117/c-kit Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit SCF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 30.0‑180 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CD117/c-kit protein
  

  Human SCFR (Gln26-Thr520) Accession # P10721.1	IEGRMD	Human IgG1 Fc (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P10721.1
• N-terminal Sequence: Gln26
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-120 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD117/c-kit Fc Chimera Protein, CF

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem cell factor receptor (SCFR), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that acts as the receptor for the cytokine SCF. Human SCFR is expressed in embryonic stem cells and is involved in regulation of cell survival and proliferation, hematopoiesis, gametogenesis, and melanogenesis (1-3). Mature human SCFR consists of an extracellular domain (ECD) with five tandem immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain. Within the ECD, human SCFR shares 73% and 76% amino acid sequence identity with mouse and rat SCFR, respectively. Several SCFR isoforms, produced by alternative mRNA splicing, have been identified in humans and can be characterized by the presence or absence of a tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (4, 5). Binding of SCFR to SCF triggers receptor dimerization and activates downstream signaling (6). SCF is a primary growth and activation factor for mast cells and eosinophils and SCFR expression on mast cells enables them to infiltrate SCF‑secreting tumors where they promote tumor growth and induce local immune suppression (7, 8). SCFR is up regulated on dendritic cells by Th2- or Th17-biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (9). SCFR protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (10, 11). Mutations or deletions of SCFR cause a wide variety of malignancies as well as pigmentation disorders and sterility (12).

1. Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
2. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
3. Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
4. Lennartsson, J. and Rönnstrand L. (2012) Physiol Rev. 92:1619.
5. Caruana, G. et al. (1999) Oncogene 18:5573.
6. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
7. Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
8. Huang, B. et al. (2008) Blood 112:1269.
9. Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
10. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
11. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
12. Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.

Bioinformatics

• Uniprot:
  • P10721.1()