Recombinant Human ACE-2 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human ACE-2 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >800 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human ACE-2 protein
Gln18-Ser740, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
No results obtained: Gln18 predicted
Structure / Form
Recombinant Human ACE‑2 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
Protein/Peptide Type
Recombinant Enzymes
Gene
ACE2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa, reducing conditions
Publications
Read Publications using
933-ZN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 75 mM Tris, 1 M NaCl, pH 7.5
  • Recombinant Human ACE-2 (rhACE-2) (Catalog # 933-ZN)
  • Substrate: MCA-Tyr-Val-Ala-Asp-Ala-Pro-Lys(DNP)-OH (Catalog # ES007), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhACE-2 to 0.2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 40 μM in Assay Buffer.
  3. Load in a black well plate 50 µL of 0.2 ng/µL rhACE-2, and start the reaction by adding 50 µL of 40 µM Substrate. As a control load 50 µL of 40 µM Substrate with 50 µL of Assay Buffer.
  4. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes.
  5. Calculate specific activity: 

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975)

Per Well:
  • rhACE-2: 0.010 µg
  • Substrate: 20 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ACE-2 Protein, CF

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

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Publications for ACE-2 (933-ZN)(9)

We have publications tested in 6 confirmed species: Human, Mouse, Canine, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Virus.

We have publications tested in 5 applications: Binding Assay, Bioassay, Enzyme Assay, Flow Cytometry, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(4)
Enzyme Assay
(2)
Flow Cytometry
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Canine
(1)
N/A
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Virus
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 933-ZN Applications Species
É Larouche-L, KA Loughran, MA Oyama, PF Solter, DS Laughlin, MD Sánchez, CA Assenmache, PR Fox, RC Fries Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease J. Vet. Intern. Med., 2019;0(0):. 2019 [PMID: 31254308] (Bioassay, Canine) Bioassay Canine
AM South, PA Nixon, MC Chappell, DI Diz, GB Russell, HA Shaltout, TM O'Shea, LK Washburn Obesity is Associated with Higher Blood Pressure and Higher Levels of Angiotensin II but Lower Angiotensin-(1-7) in Adolescents Born Preterm J. Pediatr., 2018;0(0):. 2018 [PMID: 30404738] (Bioassay, Human) Bioassay Human
Angiotensin-converting enzyme 2 is reduced in Alzheimer&#039;s disease in association with increasing amyloid-? and tau pathology Alzheimers Res Ther, 2016;8(1):50. 2016 [PMID: 27884212] (Enzyme Assay, Human) Enzyme Assay Human
Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol, 2014;88(2):1293-307. 2014 [PMID: 24227843] (Bioassay, Human) Bioassay Human
Zhou Y, Lu K, Pfefferle S, Bertram S, Glowacka I, Drosten C, Pohlmann S, Simmons G A single asparagine-linked glycosylation site of the severe acute respiratory syndrome coronavirus spike glycoprotein facilitates inhibition by mannose-binding lectin through multiple mechanisms. J. Virol., 2011;84(17):8753-64. 2011 [PMID: 20573835] (Binding Assay, Virus) Binding Assay Virus
Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system. J. Am. Coll. Cardiol., 2008;52(9):750-4. 2008 [PMID: 18718423] (Enzyme Assay, N/A) Enzyme Assay N/A
de Lang A, Osterhaus AD, Haagmans BL Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells. Virology, 2006;353(2):474-81. 2006 [PMID: 16860835] (Bioassay, Primate - Chlorocebus pygerythrus (Vervet Monkey)) Bioassay Primate - Chlorocebus pygerythrus (Vervet Monkey)
Follis KE, York J, Nunberg JH Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry. Virology, 2006;350(2):358-69. 2006 [PMID: 16519916] (Flow Cytometry, Human) Flow Cytometry Human
Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B, Yang P, Sarao R, Wada T, Leong-Poi H, Crackower MA, Fukamizu A, Hui CC, Hein L, Uhlig S, Slutsky AS, Jiang C, Penninger JM Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature, 2005;436(7047):112-6. 2005 [PMID: 16001071] (In Vivo, Mouse) In Vivo Mouse

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Bioinformatics

Gene Symbol ACE2
Uniprot