RCAN1 Antibody

Images

 
Western Blot: RCAN1 Antibody [NBP1-46852] - Western blot analysis of human skeletal muscle tissue (A) using RCAN1 antibody at 2 ug/ml.
Immunohistochemistry: RCAN1 Antibody [NBP1-46852] - Immunohistochemical analysis of RCAN1 in human fetal brain.
Western Blot: RCAN1 Antibody [NBP1-46852] - Analysis of RCAN1 in human fetal brain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

RCAN1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human RCAN1 (within residues 40-90). [Swiss-Prot# P53805]
Localization
Cytoplasm, nucleus
Specificity
This antibody is specific for isoform 1-1L.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RCAN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
This RCAN1 antibody is useful for Western blot and Immunohistochemistry on paraffin-embedded sections. In WB a band is seen at ~38 kDa representing isoform RCAN1-1L.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Fetal Whole Normal)
Control Peptide

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RCAN1 Antibody

  • ADAPT78
  • calcipressin-1
  • calcium and oxidant-inducible mRNA
  • Down syndrome candidate region 1
  • Down syndrome critical region gene 1
  • Down syndrome critical region protein 1
  • DSC1
  • modulatory calcineurin-interacting protein 1
  • Myocyte-enriched calcineurin-interacting protein 1
  • near DSCR proline-rich protein
  • regulator of calcineurin 1CSP1DSCR1MCIP1RCN1

Background

RCAN1 (regulator of calcineurin 1) inhibits the calcineurin-dependent transcriptional responses by binding to catalytic domain of calcineurin 1, a protein phosphatase involved in muscle cell differentiation/growth, T cell activation and new memory generation. Unphosphorylated RCAN1 binds/inhibits calcineurin, while RCAN1 phosphorylation at conserved serine residues interefere with this binding, resulting in increased calcineurin activity. RCAN1 over-expression mediated reduction in calcineurin activity alters the phosphorylation/activity of several calcineurin targets such as Munc18, dynamin 1, amphiphysin 1/2 and synaptojanin which are involved in exocytosis and vesicle recycling. Chronic alterations in calcineurin lead to transcriptional effects via calcineurin/NFAT signalling that regulates pre-synaptic neurotransmitter release. RCAN1 also affect exocytosis and quantal release kinetics via direct calcineurin independent mechanisms. RCAN1 is widely expressed in normal tissues/cell types with highest expression in neurons, and RCAN1 levels elevates in Down syndrome fetuses as well as Alzheimer patients, whereas, in Huntington disease RCAN1 - expression is found reduced. RCAN1 has been implicated in a variety of cellular processes, including oxidative stress, angiogenesis, mitochondrial function, immune responses and inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for RCAN1 Antibody (NBP1-46852): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RCAN1 Antibody (NBP1-46852) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-46852AF350
Alexa Fluor 405 NBP1-46852AF405
Alexa Fluor 488 NBP1-46852AF488
Alexa Fluor 532 NBP1-46852AF532
Alexa Fluor 594 NBP1-46852AF594
Alexa Fluor 647 NBP1-46852AF647
Alexa Fluor 700 NBP1-46852AF700
Alexa Fluor 750 NBP1-46852AF750
Biotin NBP1-46852B
DyLight 350 NBP1-46852UV
DyLight 405 NBP1-46852V
DyLight 488 NBP1-46852G
DyLight 550 NBP1-46852R
DyLight 594 NBP1-46852DL594
DyLight 650 NBP1-46852C
DyLight 680 NBP1-46852FR
DyLight 755 NBP1-46852IR
FITC NBP1-46852F
HRP NBP1-46852H
Janelia Fluor 549 NBP1-46852JF549
Janelia Fluor 646 NBP1-46852JF646

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Bioinformatics

Gene Symbol RCAN1
Entrez
Uniprot
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